دورية أكاديمية
TFE3/PI3K/Akt/mTOR Axis in Renal Cell Carcinoma Affects Tumor Microenvironment.
| العنوان: | TFE3/PI3K/Akt/mTOR Axis in Renal Cell Carcinoma Affects Tumor Microenvironment. |
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| المؤلفون: | Hwang C; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Kang YK; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Kim JY; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Shin SH; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Park JY; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Song JS; Department of Pathology, School of Medicine, Pusan National University, Yangsan, Korea., Kim SY; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Jung SJ; Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea., Lee JH; Department of Pathology, School of Medicine, Pusan National University, Pusan National University Yangsan Hospital, Yangsan, Korea., Na JY; Department of Pathology, School of Medicine, Pusan National University, Pusan National University Yangsan Hospital, Yangsan, Korea., Shin DH; Department of Pathology, School of Medicine, Pusan National University, Pusan National University Yangsan Hospital, Yangsan, Korea., Kim JY; Department of Pathology, School of Medicine, Pusan National University, Pusan National University Yangsan Hospital, Yangsan, Korea., Park SW; Department of Urology, School of Medicine, Pusan National University, Pusan National University Yangsan Hospital, Yangsan, Korea., Lee HJ; Department of Pathology, School of Medicine, Pusan National University, Pusan National University Yangsan Hospital, Yangsan, Korea; Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea. Electronic address: hyunjunglee@pusan.ac.kr. |
| المصدر: | The American journal of pathology [Am J Pathol] 2024 Jul; Vol. 194 (7), pp. 1306-1316. Date of Electronic Publication: 2024 Apr 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0370502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-2191 (Electronic) Linking ISSN: 00029440 NLM ISO Abbreviation: Am J Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011-: New York : Elsevier Original Publication: Philadelphia [etc.] American Assn. of Pathologists [etc.] |
| مواضيع طبية MeSH: | Carcinoma, Renal Cell*/pathology , Carcinoma, Renal Cell*/metabolism , Carcinoma, Renal Cell*/genetics , TOR Serine-Threonine Kinases*/metabolism , Tumor Microenvironment*/physiology , Kidney Neoplasms*/pathology , Kidney Neoplasms*/metabolism , Kidney Neoplasms*/genetics , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*/metabolism , Proto-Oncogene Proteins c-akt*/metabolism , Signal Transduction*/physiology, Humans ; Cell Line, Tumor ; Phosphatidylinositol 3-Kinases/metabolism ; Gene Expression Regulation, Neoplastic |
| مستخلص: | The role of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway in renal cell carcinoma (RCC) progression, metastasis, and resistance to therapies has not been investigated thoroughly. Transcription factor E3 (TFE3) expression is related to a poorer prognosis and tumor microenvironment in patients with RCC. This study aimed to determine the relationship between TFE3 and the PI3K/Akt pathway. TFE3 down-regulation was achieved by transient transfection of siRNA and shRNA in UOK146 cells. TFE3 overexpression was induced by transient transfection with pcDNA3.1 encoding the constitutively active form of TFE3. The cells were treated with mammalian target of rapamycin (mTOR) and PI3K inhibitors. Western blot was performed to detect TFE3, programmed death-ligand 1, phospho-Akt, and Akt. Phospho-Akt expression increased significantly upon TFE3 down-regulation, and decreased significantly upon up-regulation. When RCC cells were treated with a PI3K inhibitor (LY294002), TFE3 expression increased and phospho-Akt expression decreased. Data from this study indicate that TFE3 plays a role in the PI3K/Akt pathway in RCC. The results of this study suggest that PI3K/Akt inhibitors may aid in the treatment of patients with RCC by affecting the tumor microenvironment. Competing Interests: Disclosure Statement None declared. (Copyright © 2024 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (TFE3 protein, human) EC 2.7.11.1 (TOR Serine-Threonine Kinases) 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) EC 2.7.1.1 (MTOR protein, human) EC 2.7.1.- (Phosphatidylinositol 3-Kinases) |
| تواريخ الأحداث: | Date Created: 20240408 Date Completed: 20240616 Latest Revision: 20240701 |
| رمز التحديث: | 20240701 |
| DOI: | 10.1016/j.ajpath.2024.02.022 |
| PMID: | 38588851 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1525-2191 |
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| DOI: | 10.1016/j.ajpath.2024.02.022 |