دورية أكاديمية
Dynamics of the Trypanosoma cruzi infection in adipose tissue: Assessing gene expression of PNPLA2, FASN, and ACAT1 under Benzonidazole treatment and indirect mononuclear immune cells interaction.
| العنوان: | Dynamics of the Trypanosoma cruzi infection in adipose tissue: Assessing gene expression of PNPLA2, FASN, and ACAT1 under Benzonidazole treatment and indirect mononuclear immune cells interaction. |
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| المؤلفون: | da Silva AC; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Moreira LR; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil; Universidade Federal de Pernambuco, Recife, PE, Brasil., Oliveira CNDC; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Júnior CDDS; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil; Universidade Federal de Pernambuco, Recife, PE, Brasil., Ó KPD; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Oliveira KKDS; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Melo MGN; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Soares AKA; Fundação Altino Ventura, Recife, PE, Brasil., Cavalcanti MP; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Vasconcelos LRS; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil., Lorena VMB; Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil. Electronic address: virginia.lorena@fiocruz.br. |
| المصدر: | Molecular and biochemical parasitology [Mol Biochem Parasitol] 2024 Jun; Vol. 258, pp. 111618. Date of Electronic Publication: 2024 Apr 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8006324 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9428 (Electronic) Linking ISSN: 01666851 NLM ISO Abbreviation: Mol Biochem Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Leukocytes, Mononuclear*/immunology , Leukocytes, Mononuclear*/parasitology , Adipose Tissue*/parasitology , Adipose Tissue*/metabolism , Trypanosoma cruzi*/drug effects , Trypanosoma cruzi*/genetics , Lipase*/genetics , Lipase*/metabolism , Fatty Acid Synthase, Type I*/genetics , Fatty Acid Synthase, Type I*/metabolism , Acyltransferases*, Humans ; Acetyl-CoA C-Acetyltransferase/genetics ; Acetyl-CoA C-Acetyltransferase/metabolism ; Chagas Disease/drug therapy ; Chagas Disease/parasitology ; Chagas Disease/genetics ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Parasite Load ; Gene Expression ; Cells, Cultured |
| مستخلص: | Trypanosoma cruzi is a parasite with a high capacity to adapt to the host. Animal models have already demonstrated that the tropism of this parasite occurs not only in cardiac/digestive tissues but also in adipose tissue (AT). That said, the consequences ofT. cruziinfection for AT and the implications of treatment with Benzonidazole in this tissue are under discussion. Here, we tested the hypothesis that T. cruzi infection in adipose tissue upon treatment with Benzonidazole (Bz) and the interaction of mononuclear immune cells (PBMC) influences the relative expression of ACAT1, FASN, and PNPLA2 genes. Thus, stem cells derived from adipose tissue (ADSC) after adipogenic differentiation were indirectly cultivated with PBMC after infection with the T. cruzi Y strain and treatment with Bz. We use the TcSAT-IAM system and RT-qPCR to evaluate the parasite load and the relative quantification (ΔCt) of the ACAT1, FASN, and PNPLA2 genes. Our results demonstrate that treatment with Bz did not reduce adipocyte infection in the presence (p-value: 0.5796) or absence (p-value: 0.1854) of cultivation with PBMC. In addition, even though there is no statistical difference when compared to the control group (AT), T. cruzi induces the FASN expression (Rq: 14.00). However, treatment with Bz in AT suggests the increases of PNPLA2 expression levels (Rq: 12.58), even in the absence of T. cruzi infection. During indirect cultivation with PBMC, T. cruzi smooths the expression of PNPLA2 (Rq: 0.824) and instigates the expression of ACAT1 (Rq: 1.632) and FASN (Rq: 1.394). Furthermore, the treatment with Bz during infection induces PNPLA2 expression (Rq: 1.871), maintaining FASN expression levels (Rq: 1.334). Given this, our results indicate that treatment with Benzonidazole did not decrease T. cruzi infection in adipose tissue. However, treating the adipocyte cells with Bz during the interaction with PBMC cells influences the lipid pathways scenario, inducing lipolytic metabolism through the expression of PNPLA2. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ACAT1; Adipocytes; FASN; PBMC; PNPLA2; Trypanosoma cruzi |
| المشرفين على المادة: | EC 3.1.1.3 (Lipase) EC 2.3.1.85 (Fatty Acid Synthase, Type I) EC 2.3.1.85 (FASN protein, human) EC 3.1.1.3 (PNPLA2 protein, human) EC 2.3.1.9 (Acetyl-CoA C-Acetyltransferase) 0 (Membrane Proteins) EC 2.3.- (Acyltransferases) |
| تواريخ الأحداث: | Date Created: 20240408 Date Completed: 20240506 Latest Revision: 20240520 |
| رمز التحديث: | 20240520 |
| DOI: | 10.1016/j.molbiopara.2024.111618 |
| PMID: | 38588892 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-9428 |
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| DOI: | 10.1016/j.molbiopara.2024.111618 |