دورية أكاديمية
أعرض في EDS

Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis).

التفاصيل البيبلوغرافية
العنوان: Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis).
المؤلفون: Ribeiro TP; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Martins-de-Sa D; Department of Cellular Biology, University of Brasília, Brasília, DF 70910-900, Brazil; Genesilico Biotech, Brasília, DF 71503-508, Brazil., Macedo LLP; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Lourenço-Tessutti IT; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Ruffo GC; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil; Graduate Program in Genomic Science and Biotechnology, Catholic University of Brasília, Brasília, DF 71966-700, Brazil., Sousa JPA; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil; Graduate Program in Genomic Science and Biotechnology, Catholic University of Brasília, Brasília, DF 71966-700, Brazil., Rósario Santana JMD; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil; Graduate Program in Genomic Science and Biotechnology, Catholic University of Brasília, Brasília, DF 71966-700, Brazil., Oliveira-Neto OB; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil; Euroamerican University Center, Unieuro, Brasília, DF 70790-160, Brazil., Moura SM; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Silva MCM; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Morgante CV; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil; Embrapa Semi-Arid, Pretrolina, PE 56302-970, Brazil., Oliveira NG; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Basso MF; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil., Grossi-de-Sa MF; Embrapa Genetic Resources and Biotechnology, Brasília, DF 70770-917, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brasília, DF 70770-917, Brazil; Graduate Program in Genomic Science and Biotechnology, Catholic University of Brasília, Brasília, DF 71966-700, Brazil; Graduate Program in Biotechnology, Catholic University Dom Bosco, Campo Grande, MS 79117-900, Brazil. Electronic address: fatima.grossi@embrapa.br.
المصدر: Plant science : an international journal of experimental plant biology [Plant Sci] 2024 Jul; Vol. 344, pp. 112079. Date of Electronic Publication: 2024 Apr 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Ireland Country of Publication: Ireland NLM ID: 9882015 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2259 (Electronic) Linking ISSN: 01689452 NLM ISO Abbreviation: Plant Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2010>- : Shannon, Co. Clare : Elsevier Ireland
Original Publication: Shannon [Clare] : Elsevier Scientific Publishers Ireland Ltd., c1985-
مواضيع طبية MeSH: Gossypium*/genetics , Gossypium*/parasitology , Weevils*/genetics , Plants, Genetically Modified*/genetics , Endotoxins*/genetics , Endotoxins*/metabolism , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism , Bacterial Proteins*/pharmacology , Hemolysin Proteins*/genetics , Hemolysin Proteins*/metabolism , Hemolysin Proteins*/pharmacology , Bacillus thuringiensis Toxins* , Larva*/drug effects, Animals ; Bacillus thuringiensis/genetics ; Pest Control, Biological
مستخلص: The cotton boll weevil (CBW, Anthonomus grandis) stands as one of the most significant threats to cotton crops (Gossypium hirsutum). Despite substantial efforts, the development of a commercially viable transgenic cotton event for effective open-field control of CBW has remained elusive. This study describes a detailed characterization of the insecticidal toxins Cry23Aa and Cry37Aa against CBW. Our findings reveal that CBW larvae fed on artificial diets supplemented exclusively with Cry23Aa decreased larval survival by roughly by 69%, while supplementation with Cry37Aa alone displayed no statistical difference compared to the control. However, the combined provision of both toxins in the artificial diet led to mortality rates approaching 100% among CBW larvae (LC 50 equal to 0.26 PPM). Additionally, we engineered transgenic cotton plants by introducing cry23Aa and cry37Aa genes under control of the flower bud-specific pGhFS4 and pGhFS1 promoters, respectively. Seven transgenic cotton events expressing high levels of Cry23Aa and Cry37Aa toxins in flower buds were selected for greenhouse bioassays, and the mortality rate of CBW larvae feeding on their T 0 and T 1 generations ranged from 75% to 100%. Our in silico analyses unveiled that Cry23Aa displays all the hallmark characteristics of β-pore-forming toxins (β-PFTs) that bind to sugar moieties in glycoproteins. Intriguingly, we also discovered a distinctive zinc-binding site within Cry23Aa, which appears to be involved in protein-protein interactions. Finally, we discuss the major structural features of Cry23Aa that likely play a role in the toxin's mechanism of action. In view of the low LC 50 for CBW larvae and the significant accumulation of these toxins in the flower buds of both T 0 and T 1 plants, we anticipate that through successive generations of these transgenic lines, cotton plants engineered to overexpress cry23Aa and cry37Aa hold promise for effectively managing CBW infestations in cotton crops.
Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Coleoptera; Cry23Aa/Mpp23Aa; Cry37Aa/Xpp37Aa; Gossypium hirsutum; Insect pest; Insecticidal toxins; Transgenic cotton
المشرفين على المادة: 0 (insecticidal crystal protein, Bacillus Thuringiensis)
تواريخ الأحداث: Date Created: 20240408 Date Completed: 20240519 Latest Revision: 20240519
رمز التحديث: 20240520
DOI: 10.1016/j.plantsci.2024.112079
PMID: 38588981
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2259
DOI:10.1016/j.plantsci.2024.112079