دورية أكاديمية
The overlap of accessory virulence factors and multidrug resistance among clinical and surveillance Klebsiella pneumoniae isolates from a neonatal intensive care unit in Nepal: a single-centre experience in a resource-limited setting.
| العنوان: | The overlap of accessory virulence factors and multidrug resistance among clinical and surveillance Klebsiella pneumoniae isolates from a neonatal intensive care unit in Nepal: a single-centre experience in a resource-limited setting. |
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| المؤلفون: | Shrestha RK; Siddhi Memorial Hospital, Bhimsensthan-7, Bhaktapur, Nepal., Shrestha D; Siddhi Memorial Hospital, Bhimsensthan-7, Bhaktapur, Nepal., Kunwar AJ; Kathmandu Center for Genomics and Research Laboratory, Lalitpur, Nepal., Thapa S; Kathmandu Center for Genomics and Research Laboratory, Lalitpur, Nepal., Shrestha N; Siddhi Memorial Hospital, Bhimsensthan-7, Bhaktapur, Nepal., Dhoubhadel BG; School of Tropical Medicine and Global Health (TMGH), Nagasaki University, Nagasaki, 852-8523, Japan. b-gopal@nagasaki-u.ac.jp.; Department of Respiratory Infections, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan. b-gopal@nagasaki-u.ac.jp., Parry CM; School of Tropical Medicine and Global Health (TMGH), Nagasaki University, Nagasaki, 852-8523, Japan.; Clinical Sciences and Education, Liverpool School of Tropical Medicine, Liverpool, UK. |
| المصدر: | Tropical medicine and health [Trop Med Health] 2024 Apr 08; Vol. 52 (1), pp. 30. Date of Electronic Publication: 2024 Apr 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Ltd Country of Publication: Japan NLM ID: 101215093 Publication Model: Electronic Cited Medium: Print ISSN: 1348-8945 (Print) Linking ISSN: 13488945 NLM ISO Abbreviation: Trop Med Health Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: <2016- > : London : BioMed Central Ltd. Original Publication: Nagasaki, Japan : Japanese Society of Tropical Medicine, [2004]- |
| مستخلص: | Background: There is a lack of data on the characteristics of overlap between acquired antimicrobial resistance and virulence factors in Klebsiella pneumoniae in high-risk settings, especially with the inclusion of surveillance isolates along with the clinical. We investigated K. pneumoniae isolates, from a neonatal intensive care unit (NICU) in Nepal, for the presence of both accessory virulence factors and acquired antimicrobial resistance. Methods: Thirty-eight clinical and nineteen surveillance K. pneumoniae isolates obtained between January 2017 and August 2022 in the NICU of Siddhi Memorial Hospital, Bhaktapur, Nepal were investigated with antimicrobial susceptibility testing, PCR-based detection of β-lactamases and virulence factors, and genetic similarity by ERIC-PCR. Results: K. pneumoniae was found positive in 37/85 (43.5%) blood culture-positive neonatal bloodstream infections, 34/954 (3.6%) patient surveillance cultures, and 15/451 (3.3%) environmental surveillance samples. Among 57 isolates analyzed in this study, we detected multidrug resistance in 37/57 (64.9%), which was combined with at least one accessory virulence factor in 21/37 (56.8%). This overlap was mostly among β-lactamase producing isolates with accessory mechanisms of iron acquisition. These isolates displayed heterogenous ERIC-PCR patterns suggesting genetic diversity. Conclusions: The clinical significance of this overlap between acquired antimicrobial resistance and accessory virulence genes in K. pneumoniae needs further investigation. Better resource allocation is necessary to strengthen infection prevention and control interventions in resource-limited settings. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | 22K15924 Japan Society for the Promotion of Science |
| فهرسة مساهمة: | Keywords: Klebsiella pneumoniae; Extended-spectrum β-lactamase; Neonatal intensive care unit (NICU); Nepal; Virulence |
| تواريخ الأحداث: | Date Created: 20240408 Latest Revision: 20240410 |
| رمز التحديث: | 20240410 |
| مُعرف محوري في PubMed: | PMC11000294 |
| DOI: | 10.1186/s41182-024-00595-3 |
| PMID: | 38589977 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1348-8945 |
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| DOI: | 10.1186/s41182-024-00595-3 |