دورية أكاديمية
أعرض في EDS

Genetic dissection of the tissue-specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry.

التفاصيل البيبلوغرافية
العنوان: Genetic dissection of the tissue-specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry.
المؤلفون: Vadillo-Dieguez A; NIAB, Cambridge, UK.; School of Biosciences and the Birmingham Institute of Forest Research, University of Birmingham, Birmingham, UK., Zeng Z; NIAB, Cambridge, UK., Mansfield JW; Faculty of Natural Sciences, Imperial College London, London, UK., Grinberg NF; NIAB, Cambridge, UK., Lynn SC; NIAB, Cambridge, UK., Gregg A; NIAB, Cambridge, UK., Connell J; NIAB, Cambridge, UK., Harrison RJ; NIAB, Cambridge, UK.; School of Biosciences and the Birmingham Institute of Forest Research, University of Birmingham, Birmingham, UK.; Faculty of Natural Sciences, Plant Science Group, Wageningen University and Research, Wageningen, Netherlands., Jackson RW; School of Biosciences and the Birmingham Institute of Forest Research, University of Birmingham, Birmingham, UK., Hulin MT; NIAB, Cambridge, UK.; Department of Plant Soil & Microbial Sciences, Michigan State University, East Lansing, USA.
المصدر: Molecular plant pathology [Mol Plant Pathol] 2024 Apr; Vol. 25 (4), pp. e13451.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Science in collaboration with the British Society of Plant Pathology Country of Publication: England NLM ID: 100954969 Publication Model: Print Cited Medium: Internet ISSN: 1364-3703 (Electronic) Linking ISSN: 13643703 NLM ISO Abbreviation: Mol Plant Pathol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, UK : Blackwell Science in collaboration with the British Society of Plant Pathology, c2000-
مواضيع طبية MeSH: Prunus avium*/metabolism , Prunus*/metabolism, Virulence/genetics ; Pseudomonas syringae ; Fruit/metabolism ; Mutation/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
مستخلص: When compared with other phylogroups (PGs) of the Pseudomonas syringae species complex, P. syringae pv. syringae (Pss) strains within PG2 have a reduced repertoire of type III effectors (T3Es) but produce several phytotoxins. Effectors within the cherry pathogen Pss 9644 were grouped based on their frequency in strains from Prunus as the conserved effector locus (CEL) common to most P. syringae pathogens; a core of effectors common to PG2; a set of PRUNUS effectors common to cherry pathogens; and a FLEXIBLE set of T3Es. Pss 9644 also contains gene clusters for biosynthesis of toxins syringomycin, syringopeptin and syringolin A. After confirmation of virulence gene expression, mutants with a sequential series of T3E and toxin deletions were pathogenicity tested on wood, leaves and fruits of sweet cherry (Prunus avium) and leaves of ornamental cherry (Prunus incisa). The toxins had a key role in disease development in fruits but were less important in leaves and wood. An effectorless mutant retained some pathogenicity to fruit but not wood or leaves. Striking redundancy was observed amongst effector groups. The CEL effectors have important roles during the early stages of leaf infection and possibly acted synergistically with toxins in all tissues. Deletion of separate groups of T3Es had more effect in P. incisa than in P. avium. Mixed inocula were used to complement the toxin mutations in trans and indicated that strain mixtures may be important in the field. Our results highlight the niche-specific role of toxins in P. avium tissues and the complexity of effector redundancy in the pathogen Pss 9644.
(© 2024 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
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معلومات مُعتمدة: BB/P006272/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/T010746/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
فهرسة مساهمة: Keywords: Prunus; Pseudomonas syringae; comparative genomics; mutagenesis; phytotoxins; type 3 effectors; virulence
المشرفين على المادة: 0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20240409 Date Completed: 20240410 Latest Revision: 20240411
رمز التحديث: 20240411
مُعرف محوري في PubMed: PMC11002349
DOI: 10.1111/mpp.13451
PMID: 38590135
قاعدة البيانات: MEDLINE
الوصف
تدمد:1364-3703
DOI:10.1111/mpp.13451