دورية أكاديمية
The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method.
العنوان: | The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method. |
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المؤلفون: | Lossouarn J; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Beurrier E; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Bouteau A; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Moncaut E; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Sir Silmane M; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Portalier H; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Zouari A; CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière et CNR de la Résistance aux Antibiotiques (laboratoire associé 'Entérocoques'), Rennes, France., Cattoir V; CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière et CNR de la Résistance aux Antibiotiques (laboratoire associé 'Entérocoques'), Rennes, France.; Université de Rennes, INSERM, UMR_S1230 BRM, Rennes, France., Serror P; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Petit M-A; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France. |
المصدر: | Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2024 May 02; Vol. 68 (5), pp. e0143923. Date of Electronic Publication: 2024 Apr 09. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 0315061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-6596 (Electronic) Linking ISSN: 00664804 NLM ISO Abbreviation: Antimicrob Agents Chemother Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology |
مواضيع طبية MeSH: | Enterococcus faecium*/drug effects , Bacteriophages*/genetics , Vancomycin-Resistant Enterococci*/drug effects , Host Specificity*, Phage Therapy/methods ; Gram-Positive Bacterial Infections/microbiology ; Vancomycin Resistance ; Vancomycin/pharmacology ; Humans ; Anti-Bacterial Agents/pharmacology |
مستخلص: | Phage therapy has (re)emerged as a serious possibility for combating multidrug-resistant bacterial infections, including those caused by vancomycin-resistant Enterococcus faecium strains. These opportunistic pathogens belong to a specific clonal complex 17, against which relatively few phages have been screened. We isolated a collection of 21 virulent phages growing on these vancomycin-resistant isolates. Each of these phages harbored a typical narrow plaquing host range, lysing at most 5 strains and covering together 10 strains of our panel of 14 clinical isolates. To enlarge the host spectrum of our phages, the Appelmans protocol was used. We mixed four out of our most complementary phages in a cocktail that we iteratively grew on eight naive strains from our panel, of which six were initially refractory to at least three of the combined phages. Fifteen successive passages permitted to significantly improve the lytic activity of the cocktail, from which phages with extended host ranges within the E. faecium species could be isolated. A single evolved phage able to kill up to 10 of the 14 initial E. faecium strains was obtained, and it barely infected nearby species. All evolved phages had acquired point mutations or a recombination event in the tail fiber genetic region, suggesting these genes might have driven phage evolution by contributing to their extended host spectra. Competing Interests: The authors declare no conflict of interest. |
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فهرسة مساهمة: | Keywords: Appelmans protocol; Enterococcus faecium; host range extension; vancomycin-resistant enterococci; virulent phages |
المشرفين على المادة: | 6Q205EH1VU (Vancomycin) 0 (Anti-Bacterial Agents) |
تواريخ الأحداث: | Date Created: 20240409 Date Completed: 20240502 Latest Revision: 20240629 |
رمز التحديث: | 20240629 |
مُعرف محوري في PubMed: | PMC11210271 |
DOI: | 10.1128/aac.01439-23 |
PMID: | 38591854 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1098-6596 |
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DOI: | 10.1128/aac.01439-23 |