Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels.

التفاصيل البيبلوغرافية
العنوان:	Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels.
المؤلفون:	Tan YH; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Wang KCW; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; Telethon Kids Institute, The University of Western Australia, Nedlands, WA, 6009, Australia., Chin IL; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Sanderson RW; BRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Perth, WA, 6009, Australia.; Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Perth, WA, 6009, Australia., Li J; BRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Perth, WA, 6009, Australia.; Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Perth, WA, 6009, Australia., Kennedy BF; BRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Perth, WA, 6009, Australia.; Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Perth, WA, 6009, Australia.; Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, Grudziadzka 5, Torun, 87-100, Poland., Noble PB; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Choi YS; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia.
المصدر:	Advanced healthcare materials [Adv Healthc Mater] 2024 Jul; Vol. 13 (18), pp. e2304254. Date of Electronic Publication: 2024 Apr 15.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101581613 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2192-2659 (Electronic) Linking ISSN: 21922640 NLM ISO Abbreviation: Adv Healthc Mater Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Weinheim : Wiley-VCH, 2012-
مواضيع طبية MeSH:	Hydrogels/chemistry , Hydrogels/pharmacology , Myocytes, Smooth Muscle/metabolism , Myocytes, Smooth Muscle/cytology , Myocytes, Smooth Muscle*/drug effects, Humans ; Animals ; Mechanotransduction, Cellular/physiology ; Swine ; Extracellular Matrix/metabolism ; Cells, Cultured
مستخلص:	In obstructive airway diseases such as asthma and chronic obstructive pulmonary disease (COPD), the extracellular matrix (ECM) protein amount and composition of the airway smooth muscle (ASM) is often remodelled, likely altering tissue stiffness. The underlying mechanism of how human ASM cell (hASMC) mechanosenses the aberrant microenvironment is not well understood. Physiological stiffnesses of the ASM were measured by uniaxial compression tester using porcine ASM layers under 0, 5 and 10% longitudinal stretch above in situ length. Linear stiffness gradient hydrogels (230 kPa range) were fabricated and functionalized with ECM proteins, collagen I (ColI), fibronectin (Fn) and laminin (Ln), to recapitulate the above-measured range of stiffnesses. Overall, hASMC mechanosensation exhibited a clear correlation with the underlying hydrogel stiffness. Cell size, nuclear size and contractile marker alpha-smooth muscle actin (αSMA) expression showed a strong correlation to substrate stiffness. Mechanosensation, assessed by Lamin-A intensity and nuc/cyto YAP, exhibited stiffness-mediated behaviour only on ColI and Fn-coated hydrogels. Inhibition studies using blebbistatin or Y27632 attenuated most mechanotransduction-derived cell morphological responses, αSMA and Lamin-A expression and nuc/cyto YAP (blebbistatin only). This study highlights the interplay and complexities between stiffness and ECM protein type on hASMC mechanosensation, relevant to airway remodelling in obstructive airway diseases. (© 2024 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
References:	a) J. C. Hogg, Lancet 2004, 364, 709. b) A. L. James, T. R. Bai, T. Mauad, M. J. Abramson, M. Dolhnikoff, K. O. McKay, P. S. Maxwell, J. G. Elliot, F. H. Green, Eur. Respir. J. 2009, 34, 1040. a) J. C. Hogg, F. Chu, S. Utokaparch, R. Woods, W. M. Elliott, L. Buzatu, R. M. Cherniack, R. M. Rogers, F. C. Sciurba, H. O. Coxson, P. D. Pare, N. Engl. J. Med. 2004, 350, 2645. b) A. L. James, S. Wenzel, Eur. Respir. J. 2007, 30, 134. c) P. B. Noble, R. L. Jones, A. Cairncross, J. G. Elliot, H. W. Mitchell, A. L. James, P. K. McFawn, J. Appl. Physiol. 2013, 114, 1460. a) A. L. James, J. G. Elliot, R. L. Jones, M. L. Carroll, T. Mauad, T. R. Bai, M. J. Abramson, K. O. McKay, F. H. Green, Am J. Respir. Crit. Care Med. 2012, 185, 1058. b) J. K. Burgess, T. Mauad, G. Tjin, J. C. Karlsson, G. Westergren‐Thorsson, J. Pathol. 2016, 240, 397. c) R. L. Jones, P. B. Noble, J. G. Elliot, H. W. Mitchell, P. K. McFawn, J. C. Hogg, A. L. James, Eur. Respir. J. 2016, 47, 1855. d) B. B. Araujo, M. Dolhnikoff, L. F. Silva, J. Elliot, J. H. Lindeman, D. S. Ferreira, A. Mulder, H. A. Gomes, S. M. Fernezlian, A. James, T. Mauad, Eur. Respir. J. 2008, 32, 61. R. Annoni, T. Lancas, R. Yukimatsu Tanigawa, M. de Medeiros Matsushita, S. de Morais Fernezlian, A. Bruno, L. Fernando Ferraz da Silva, P. J. Roughley, S. Battaglia, M. Dolhnikoff, P. S. Hiemstra, P. J. Sterk, K. F. Rabe, T. Mauad, Eur. Respir. J. 2012, 40, 1362. a) R. R. Jamieson, S. E. Stasiak, S. R. Polio, R. D. Augspurg, C. A. McCormick, J. W. Ruberti, H. Parameswaran, J. Appl. Physiol. 1985 2021, 130, 1635. b) E. S. White, Ann. Am. Thorac. Soc. 2015, 12, S30. S. J. Gunst, W. Zhang, Am. J. Physiol. Cell Physiol. 2008, 295, C576. a) J. V. Bonacci, T. Harris, A. G. Stewart, Clin. Exp. Pharmacol. Physiol. 2003, 30, 324. b) B. G. Dekkers, D. Schaafsma, S. A. Nelemans, J. Zaagsma, H. Meurs, Am. J. Physiol.: Lung Cell. Mol. Physiol. 2007, 292, L1405;. c) S. J. Hirst, C. H. Twort, T. H. Lee, Am. J. Respir. Cell Mol. Biol. 2000, 23, 335. d) P. R. Johnson, J. K. Burgess, P. A. Underwood, W. Au, M. H. Poniris, M. Tamm, Q. Ge, M. Roth, J. L. Black, J. Allergy Clin. Immunol. 2004, 113, 690. a) K. Parameswaran, K. Radford, J. Zuo, L. J. Janssen, P. M. O'Byrne, P. G. Cox, Eur. Respir. J. 2004, 24, 545. b) M. L. D'Antoni, P. A. Risse, P. Ferraro, J. G. Martin, M. S. Ludwig, Matrix Biol. 2012, 31, 101. A. M. Freyer, S. R. Johnson, I. P. Hall, Am. J. Respir. Cell Mol. Biol. 2001, 25, 569. J. E. Bourke, X. Li, S. R. Foster, E. Wee, H. Dagher, J. Ziogas, T. Harris, J. V. Bonacci, A. G. Stewart, Eur. Respir. J. 2011, 37, 173. a) C. Kim, J. L. Young, A. W. Holle, K. Jeong, L. G. Major, J. H. Jeong, Z. M. Aman, D. W. Han, Y. Hwang, J. P. Spatz, Y. S. Choi, Ann. Biomed. Eng. 2020, 48, 893. b) W. J. Hadden, Y. S. Choi, J. Biol. Eng. 2016, 10, 16. c) W. J. Hadden, J. L. Young, A. W. Holle, M. L. McFetridge, D. Y. Kim, P. Wijesinghe, H. Taylor‐Weiner, J. H. Wen, A. R. Lee, K. Bieback, B. N. Vo, D. D. Sampson, B. F. Kennedy, J. P. Spatz, A. J. Engler, Y. S. Choi, Proc. Natl. Acad. Sci. U. S. A. 2017, 114, 5647. d) L. G. Major, Y. S. Choi, J. Tissue Eng. Regener. Med. 2018, 12, 2021;. e) L. G. Major, A. W. Holle, J. L. Young, M. S. Hepburn, K. Jeong, I. L. Chin, R. W. Sanderson, J. H. Jeong, Z. M. Aman, B. F. Kennedy, Y. Hwang, D. W. Han, H. W. Park, K. L. Guan, J. P. Spatz, Y. S. Choi, ACS Appl. Mater. Interfaces 2019, 11, 45520. S. E. Stasiak, R. R. Jamieson, J. Bouffard, E. J. Cram, H. Parameswaran, Sci. Adv. 2020, 6, aba1149. S. R. Polio, S. E. Stasiak, R. R. Jamieson, J. L. Balestrini, R. Krishnan, H. Parameswaran, Sci. Rep. 2019, 9, 9564. A. M. Freyer, C. K. Billington, R. B. Penn, I. P. Hall, Am. J. Respir. Cell Mol. Biol. 2004, 31, 440. Q. Peng, D. Lai, T. T. Nguyen, V. Chan, T. Matsuda, S. J. Hirst, J. Immunol. 2005, 174, 2258. a) S. S. An, J. Kim, K. Ahn, X. Trepat, K. J. Drake, S. Kumar, G. Ling, C. Purington, T. Rangasamy, T. W. Kensler, W. Mitzner, J. J. Fredberg, S. Biswal, Biochem. Biophys. Res. Commun. 2009, 382, 697. b) A. Shkumatov, M. Thompson, K. M. Choi, D. Sicard, K. Baek, D. H. Kim, D. J. Tschumperlin, Y. S. Prakash, H. Kong, Am. J. Physiol.: Lung Cell. Mol. Physiol. 2015, 308, L1125. a) P. B. Noble, P. K. McFawn, H. W. Mitchell, J. Appl. Physiol. 2007, 103, 787. b) P. B. Noble, A. Sharma, P. K. McFawn, H. W. Mitchell, Eur. Respir. J. 2005, 26, 804. c) T. K. Ansell, P. K. McFawn, R. A. McLaughlin, D. D. Sampson, P. R. Eastwood, D. R. Hillman, H. W. Mitchell, P. B. Noble, J. Appl. Physiol. 2015, 118, 533. d) T. K. Ansell, P. K. McFawn, H. W. Mitchell, P. B. Noble, J. Appl. Physiol. 2013, 115, 505. e) T. K. Ansell, P. K. McFawn, P. B. Noble, A. R. West, L. Fernandes, H. W. Mitchell, Eur. Respir. J. 2009, 33, 844. f) T. K. Ansell, H. W. Mitchell, P. K. McFawn, P. B. Noble, Respirology 2016, 21, 1041. g) T. K. Ansell, P. B. Noble, H. W. Mitchell, P. K. McFawn, Br. J. Pharmacol. 2014, 171, 4376. h) A. Cairncross, P. B. Noble, P. K. McFawn, Respirology 2018, 23, 750. P. H. Wu, D. R. Aroush, A. Asnacios, W. C. Chen, M. E. Dokukin, B. L. Doss, P. Durand‐Smet, A. Ekpenyong, J. Guck, N. V. Guz, P. A. Janmey, J. S. H. Lee, N. M. Moore, A. Ott, Y. C. Poh, R. Ros, M. Sander, I. Sokolov, J. R. Staunton, N. Wang, G. Whyte, D. Wirtz, Nat. Methods 2018, 15, 491. I. L. Chin, L. Hool, Y. S. Choi, Biomater. Sci. 2021, 9, 6795. C. Y. Yick, D. S. Ferreira, R. Annoni, J. H. von der Thusen, P. W. Kunst, E. H. Bel, R. Lutter, T. Mauad, P. J. Sterk, Allergy 2012, 67, 552. A. R. Kranenburg, A. Willems‐Widyastuti, W. J. Moori, P. J. Sterk, V. K. Alagappan, W. I. de Boer, H. S. Sharma, Am. J. Clin. Pathol. 2006, 126, 725. a) N. Rosenblatt, S. Hu, B. Suki, N. Wang, D. Stamenovic, Ann. Biomed. Eng. 2007, 35, 224. b) L. Deng, N. J. Fairbank, B. Fabry, P. G. Smith, G. N. Maksym, Am. J. Physiol. Cell Physiol. 2004, 287, C440. c) N. J. Fairbank, S. C. Connolly, J. D. Mackinnon, K. Wehry, L. Deng, G. N. Maksym, Am. J. Physiol.: Lung Cell. Mol. Physiol. 2008, 295, L479. d) N. Takahara, S. Ito, K. Furuya, K. Naruse, H. Aso, M. Kondo, M. Sokabe, Y. Hasegawa, Am. J. Respir. Cell Mol. Biol. 2014, 51, 772. T. T. Nguyen, J. P. Ward, S. J. Hirst, Am J. Respir. Crit. Care Med. 2005, 171, 217. S. S. An, W. Mitzner, W. Y. Tang, K. Ahn, A. R. Yoon, J. Huang, O. Kilic, H. M. Yong, J. W. Fahey, S. Kumar, S. Biswal, S. T. Holgate, R. A. Panettieri Jr., J. Solway, S. B. Liggett, J. Allergy Clin. Immunol. 2016, 138, 294. W. Zhang, Y. Wu, S. J. Gunst, Physiol. Rev. 2023, 103, 2321. a) A. J. Halayko, B. Camoretti‐Mercado, S. M. Forsythe, J. E. Vieira, R. W. Mitchell, M. E. Wylam, M. B. Hershenson, J. Solway, Am. J. Physiol. 1999, 276, L197;. b) P. G. Woodruff, Proc. Am. Thorac. Soc. 2008, 5, 113. X. H. Zhao, C. Laschinger, P. Arora, K. Szaszi, A. Kapus, C. A. McCulloch, J. Cell Sci. 2007, 120, 1801. J. Swift, I. L. Ivanovska, A. Buxboim, T. Harada, P. C. Dingal, J. Pinter, J. D. Pajerowski, K. R. Spinler, J. W. Shin, M. Tewari, F. Rehfeldt, D. W. Speicher, D. E. Discher, Science 2013, 341, 1240104. N. Takeda, M. Kondo, S. Ito, Y. Ito, K. Shimokata, H. Kume, Am. J. Respir. Cell Mol. Biol. 2006, 35, 722. a) L. S. Velasquez, L. B. Sutherland, Z. Liu, F. Grinnell, K. E. Kamm, J. W. Schneider, E. N. Olson, E. M. Small, Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 16850. b) Z. Wang, D. Z. Wang, G. C. Pipes, E. N. Olson, Proc. Natl. Acad. Sci. U. S. A. 2003, 100, 7129. a) K. L. Du, H. S. Ip, J. Li, M. Chen, F. Dandre, W. Yu, M. M. Lu, G. K. Owens, M. S. Parmacek, Mol. Cell. Biol. 2003, 23, 2425. b) S. Li, D. Z. Wang, Z. Wang, J. A. Richardson, E. N. Olson, Proc. Natl. Acad. Sci. U. S. A. 2003, 100, 9366. J. Swift, D. E. Discher, J. Cell Sci. 2014, 127, 3005. a) S. Dupont, Exp. Cell Res. 2016, 343, 42. b) S. Dupont, L. Morsut, M. Aragona, E. Enzo, S. Giulitti, M. Cordenonsi, F. Zanconato, J. L.e Digabel, M. Forcato, S. Bicciato, N. Elvassore, S. Piccolo, Nature 2011, 474, 179. c) G. Halder, S. Dupont, S. Piccolo, Nat. Rev. Mol. Cell Biol. 2012, 13, 591. J. K. Kim, A. Louhghalam, G. Lee, B. W. Schafer, D. Wirtz, D. H. Kim, Nat. Commun. 2017, 8, 2123. J. Zhou, F. Xu, J. J. Yu, W. Zhang, Int. J. Clin. Exp. Pathol. 2015, 8, 11132. J. Fu, M. Zheng, X. Zhang, Y. Zhang, Y. Chen, H. Li, X. Wang, J. Zhang, Biochem. Biophys. Res. Commun. 2017, 493, 985. a) T. Tran, K. D. McNeill, W. T. Gerthoffer, H. Unruh, A. J. Halayko, Respir. Res. 2006, 7, 117. b) T. Tran, C. M. Teoh, J. K. Tam, Y. Qiao, C. Y. Chin, O. K. Chong, A. G. Stewart, T. Harris, W. S. Wong, S. P. Guan, B. P. Leung, W. T. Gerthoffer, H. Unruh, A. J. Halayko, FASEB J. 2013, 27, 3991. c) T. Tran, K. Ens‐Blackie, E. S. Rector, G. L. Stelmack, K. D. McNeill, G. Tarone, W. T. Gerthoffer, H. Unruh, A. J. Halayko, Am. J. Respir. Cell Mol. Biol. 2007, 37, 668. d) B. G. Dekkers, D. Schaafsma, T. Tran, J. Zaagsma, H. Meurs, Am. J. Respir. Cell Mol. Biol. 2009, 41, 494. Z. Kechagia, P. Saez, M. Gomez‐Gonzalez, B. Canales, S. Viswanadha, M. Zamarbide, I. Andreu, T. Koorman, A. E. M. Beedle, A. Elosegui‐Artola, P. W. B. Derksen, X. Trepat, M. Arroyo, P. Roca‐Cusachs, Nat. Mater. 2023, 22, 1409. W. Zhang, S. J. Gunst, J. Physiol. 2017, 595, 4279. a) L. Liu, C. Zhai, Y. Pan, Y. Zhu, W. Shi, J. Wang, X. Yan, X. Su, Y. Song, L. Gao, M. Li, Am. J. Physiol.: Lung Cell. Mol. Physiol. 2018, 315, L609. b) X. Q. Wang, L. J. Mao, Q. H. Fang, T. Kobayashi, H. J. Kim, H. Sugiura, S. Kawasaki, S. Togo, K. Kamio, X. Liu, S. I. Rennard, Prostaglandins Other Lipid Mediators 2014, 108, 23. c) S. S. Possa, H. T. Charafeddine, R. F. Righetti, P. A. da Silva, R. Almeida‐Reis, B. M. Saraiva‐Romanholo, A. Perini, C. M. Prado, E. A. Leick‐Maldonado, M. A. Martins, F. Tiberio Ide, Am. J. Physiol.: Lung Cell. Mol. Physiol. 2012, 303, L939. d) W. Zhang, B. P. Bhetwal, S. J. Gunst, J. Physiol. 2018, 596, 3617. Y. Zhang, A. Saradna, R. Ratan, X. Ke, W. Tu, D. C. Do, C. Hu, P. Gao, Clin. Transl. Immunol. 2020, 9, 01134. a) A. D. Bershadsky, N. Q. Balaban, B. Geiger, Annu. Rev. Cell Dev. Biol. 2003, 19, 677. b) D. E. Ingber, Proc. Natl. Acad. Sci. U. S. A. 2003, 100, 1472. J. M. Goffin, P. Pittet, G. Csucs, J. W. Lussi, J. J. Meister, B. Hinz, J. Cell Biol. 2006, 172, 259. R. L. Jones, P. B. Noble, J. G. Elliot, A. L. James, Respirology 2016, 21, 1347. Y. S. Choi, L. G. Vincent, A. R. Lee, K. C. Kretchmer, S. Chirasatitsin, M. K. Dobke, A. J. Engler, Biomaterials 2012, 33, 6943. A. Cairncross, P. B. Noble, P. K. McFawn, Respirology 2018, 23, 750. J. R. Tse, A. J. Engler, Curr. Protoc. Cell. Biol. 2010, 47.
معلومات مُعتمدة:	Australian Research Council; Australian Government Research Training Program Scholarship; Australian Research Council Industrial Transformation Training Centre; Western Australian Future Health and Innovation Fund, Government of Western Australia; Department of Health, Government of Western Australia
فهرسة مساهمة:	Keywords: YAP/TAZ; alpha‐smooth muscle actins; extracellular matrix; mechanotransductions; rigidity
المشرفين على المادة:	0 (Hydrogels)
تواريخ الأحداث:	Date Created: 20240409 Date Completed: 20240717 Latest Revision: 20240717
رمز التحديث:	20240717
DOI:	10.1002/adhm.202304254
PMID:	38593989
قاعدة البيانات:	MEDLINE

View record at Wiley

Full Text Finder

الوصف
تدمد:	2192-2659
DOI:	10.1002/adhm.202304254