دورية أكاديمية
Preliminary exploration of animal models of congenital choledochal cysts.
| العنوان: | Preliminary exploration of animal models of congenital choledochal cysts. |
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| المؤلفون: | Zhang SH; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Zhang YB; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Cai DT; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Pan T; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Chen K; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Jin Y; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Luo WJ; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Huang ZW; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Chen QJ; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China., Gao ZG; Department of General Surgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang Province, China. ebwk@zju.edu.cn. |
| المصدر: | World journal of gastroenterology [World J Gastroenterol] 2024 Mar 14; Vol. 30 (10), pp. 1420-1430. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Baishideng Publishing Group Country of Publication: United States NLM ID: 100883448 Publication Model: Print Cited Medium: Internet ISSN: 2219-2840 (Electronic) Linking ISSN: 10079327 NLM ISO Abbreviation: World J Gastroenterol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group Original Publication: Beijing : WJG Press, c1998- |
| مواضيع طبية MeSH: | Choledochal Cyst*/surgery, Humans ; Female ; Rats ; Animals ; Reproducibility of Results ; Rats, Sprague-Dawley ; Models, Animal ; Dilatation, Pathologic ; Bilirubin ; Disease Models, Animal |
| مستخلص: | Background: Various animal models have been used to explore the pathogenesis of choledochal cysts (CCs), but with little convincing results. Current surgical techniques can achieve satisfactory outcomes for treatment of CCs. Consequently, recent studies have focused more on clinical issues rather than basic research. Therefore, we need appropriate animal models to further basic research. Aim: To establish an appropriate animal model that may contribute to the investigation of the pathogenesis of CCs. Methods: Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated to a surgical group, sham surgical group, or control group. A rat model of CC was established by partial ligation of the bile duct. The reliability of the model was confirmed by measurements of serum biochemical indices, morphology of common bile ducts of the rats as well as molecular biology experiments in rat and human tissues. Results: Dilation classified as mild (diameter, ≥ 1 mm to < 3 mm), moderate (≥ 3 mm to < 10 mm), and severe (≥ 10 mm) was observed in 17, 17, and 2 rats in the surgical group, respectively, while no dilation was observed in the control and sham surgical groups. Serum levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, and total bile acids were significantly elevated in the surgical group as compared to the control group 7 d after surgery, while direct bilirubin, total bilirubin, and gamma-glutamyltransferase were further increased 14 d after surgery. Most of the biochemical indices gradually decreased to normal ranges 28 d after surgery. The protein expression trend of signal transducer and activator of transcription 3 in rat model was consistent with the human CC tissues. Conclusion: The model of partial ligation of the bile duct of juvenile rats could morphologically simulate the cystic or fusiform CC, which may contribute to investigating the pathogenesis of CC. Competing Interests: Conflict-of-interest statement: The authors declare that they have no conflict of interest. (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.) |
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| فهرسة مساهمة: | Keywords: Animal model; Choledochal cyst; Cystic and fusiform dilation; Juvenile rats; Partial ligation |
| المشرفين على المادة: | RFM9X3LJ49 (Bilirubin) |
| تواريخ الأحداث: | Date Created: 20240410 Date Completed: 20240411 Latest Revision: 20240411 |
| رمز التحديث: | 20240411 |
| مُعرف محوري في PubMed: | PMC11000093 |
| DOI: | 10.3748/wjg.v30.i10.1420 |
| PMID: | 38596496 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2219-2840 |
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| DOI: | 10.3748/wjg.v30.i10.1420 |