دورية أكاديمية
DNA damage in inhabitants exposed to heavy metals near Hudiara drain, Lahore, Pakistan.
| العنوان: | DNA damage in inhabitants exposed to heavy metals near Hudiara drain, Lahore, Pakistan. |
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| المؤلفون: | Jadoon S; Directorate of Curriculum and Teaching Education, Abbottabad, Pakistan. Saima.jadoon95@gmail.com.; Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan. Saima.jadoon95@gmail.com., Ali Q; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan. saim1692@gmail.com., Sami A; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan., Haider MZ; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan., Ashfaq M; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan., Javed MA; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan., Khan MA; Department of Physiology, Rashid Latif Medical College, Lahore, 54000, Pakistan. |
| المصدر: | Scientific reports [Sci Rep] 2024 Apr 10; Vol. 14 (1), pp. 8408. Date of Electronic Publication: 2024 Apr 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Soil Pollutants*/metabolism , Drinking Water*/analysis , Metals, Heavy*/analysis, Adult ; Humans ; Animals ; Cattle ; Creatinine/analysis ; Pakistan ; Cadmium/analysis ; Environmental Monitoring/methods ; Heavy Metal Poisoning ; Soil/chemistry ; Vegetables/metabolism ; DNA Damage ; DNA ; Goats/metabolism ; Risk Assessment |
| مستخلص: | The current study was conducted on the inhabitants living in the area adjacent to the Hudiara drain using bore water and vegetables adjacent to the Hudiara drain. Toxic heavy metals badly affect human health because of industrial environmental contamination. Particularly hundreds of millions of individuals globally have faced the consequences of consuming water and food tainted with pollutants. Concentrations of heavy metals in human blood were elevated in Hudiara drainings in Lahore city, Pakistan, due to highly polluted industrial effluents. The study determined the health effects of high levels of heavy metals (Cd, Cu, Zn, Fe, Pb, Ni, Hg, Cr) on residents of the Hudiara draining area, including serum MDA, 8-Isoprostane, 8-hydroxyguanosine, and creatinine levels. An absorption spectrophotometer was used to determine heavy metals in wate water, drinking water, soil, plants and human beings blood sampleas and ELISA kits were used to assess the level of 8-hydroxyguanosine, MDA, 8-Isoprostane in plasma serum creatinine level. Waste water samples, irrigation water samples, drinking water samples, Soil samples, Plants samples and blood specimens of adult of different weights and ages were collected from the polluted area of the Hudiara drain (Laloo and Mohanwal), and control samples were obtained from the unpolluted site Sheiikhpura, 60 km away from the site. Toxic heavy metals in blood damage the cell membrane and DNA structures, increasing the 8-hydroxyguanosine, MDA, creatinine, and 8-Isoprostane. Toxic metals contaminated bore water and vegetables, resulting in increased levels of creatinine, MDA, Isoprostane, and 8-hydroxy-2-guanosine in the blood of inhabitants from the adjacent area Hudiara drain compared to the control group. In addition,. This study also investigated heavy metal concentrations in meat and milk samples from buffaloes, cows, and goats. In meat, cow samples showed the highest Cd, Cu, Fe and Mn concentrations. In milk also, cows exhibited elevated Cu and Fe levels compared to goats. The results highlight species-specific variations in heavy metal accumulation, emphasizing the need for targeted monitoring to address potential health risks. The significant difference between the two groups i.e., the control group and the affected group, in all traits of the respondents (weight, age, heavy metal values MDA, 8-Isoprostane, 8-hydroxyguaniosine, and serum creatinine level). Pearson's correlation coefficient was calculated. The study has shown that the level of serum MDA, 8-Isoprostane, 8-hydroxyguaniosine, or creatinine has not significantly correlated with age, so it is independent of age. This study has proved that in Pakistan, the selected area of Lahore in the villages of Laloo and Mohanwal, excess of heavy metals in the human body damages the DNA and increases the level of 8-Isoprostane, MDA, creatinine, and 8-hydroxyguaniosine. As a result, National and international cooperation must take major steps to control exposure to heavy metals. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: 8-Isoprostane; 8-hydroxyguanosine; DNA damage; Heavy metals; MDA |
| المشرفين على المادة: | AYI8EX34EU (Creatinine) 0 (Soil Pollutants) 0 (Drinking Water) 00BH33GNGH (Cadmium) 0 (Metals, Heavy) 0 (Soil) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20240410 Date Completed: 20240412 Latest Revision: 20240425 |
| رمز التحديث: | 20240425 |
| مُعرف محوري في PubMed: | PMC11006874 |
| DOI: | 10.1038/s41598-024-58655-x |
| PMID: | 38600156 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-024-58655-x |