دورية أكاديمية
أعرض في EDS

Exposure of chimaeric embryos to exogenous FGF4 leads to the production of pure ESC-derived mice.

التفاصيل البيبلوغرافية
العنوان: Exposure of chimaeric embryos to exogenous FGF4 leads to the production of pure ESC-derived mice.
المؤلفون: Soszyńska A; Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: a.kasperczuk@student.uw.edu.pl., Krawczyk K; Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: k.klimczewska@uw.edu.pl., Szpila M; Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: marcin.szpila@uw.edu.pl., Winek E; Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: e.winek@student.uw.edu.pl., Szpakowska A; Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: am.szpakowsk@student.uw.edu.pl., Suwińska A; Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: a.suwinska@uw.edu.pl.
المصدر: Theriogenology [Theriogenology] 2024 Jul 01; Vol. 222, pp. 10-21. Date of Electronic Publication: 2024 Apr 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE
أسماء مطبوعة: Publication: [New York, N.Y.?] : Elsevier
Original Publication: Los Altos, Calif., Geron-X.
مواضيع طبية MeSH: Fibroblast Growth Factor 4*/genetics , Chimera*, Animals ; Mice ; Embryonic Stem Cells ; Mice, Transgenic ; Embryo, Mammalian ; Cell Differentiation
مستخلص: Producing chimaeras constitutes the most reliable method of verifying the pluripotency of newly established cells. Moreover, forming chimaeras by injecting genetically modified embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) into the embryo is part of the procedure for generating transgenic mice, which are used for understanding gene function. Conventional methods for generating transgenic mice, including the breeding of chimaeras and tetraploid complementation, are time-consuming and cost-inefficient, with significant limitations that hinder their effectiveness and widespread applications. In the present study, we modified the traditional method of chimaera generation to significantly speed up this process by generating mice exclusively derived from ESCs. This study aimed to assess whether fully ESC-derived mice could be obtained by modulating fibroblast growth factor 4 (FGF4) levels in the culture medium and changing the direction of cell differentiation in the chimaeric embryo. We found that exogenous FGF4 directs all host blastomeres to the primitive endoderm fate, but does not affect the localisation of ESCs in the epiblast of the chimaeric embryos. Consequently, all FGF4-treated chimaeric embryos contained an epiblast composed exclusively of ESCs, and following transfer into recipient mice, these embryos developed into fully ESC-derived newborns. Collectively, this simple approach could accelerate the generation of ESC-derived animals and thus optimise ESC-mediated transgenesis and the verification of cell pluripotency. Compared to traditional methods, it could speed up functional studies by several weeks and significantly reduce costs related to maintaining and breeding chimaeras. Moreover, since the effect of stimulating the FGF signalling pathway is universal across different animal species, our approach can be applied not only to rodents but also to other animals, offering its utility beyond laboratory settings.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Blastocyst; Chimera; Embryonic stem cell; FGF4; Preimplantation embryo
المشرفين على المادة: 0 (Fibroblast Growth Factor 4)
0 (Fgf4 protein, mouse)
تواريخ الأحداث: Date Created: 20240411 Date Completed: 20240426 Latest Revision: 20240426
رمز التحديث: 20240427
DOI: 10.1016/j.theriogenology.2024.03.017
PMID: 38603966
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3231
DOI:10.1016/j.theriogenology.2024.03.017