دورية أكاديمية
أعرض في EDS

Sexual Dimorphism's impact on adipogenesis: A three-dimensional in vitro model treated with 17β-estradiol and testosterone.

التفاصيل البيبلوغرافية
العنوان: Sexual Dimorphism's impact on adipogenesis: A three-dimensional in vitro model treated with 17β-estradiol and testosterone.
المؤلفون: Pal P; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA., Maranon RO; Department of Physiology, Faculty of Medicine, Universidad Nacional de Tucuman, San Miguel de Tucumán, Argentina; National Council on Scientific and Technical Research (CONICET), Tucuman, Argentina., Rivera Gonzales OJ; Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA., Speed JS; Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA., Janorkar AV; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA. Electronic address: ajanorkar@umc.edu.
المصدر: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 Aug 01; Vol. 589, pp. 112249. Date of Electronic Publication: 2024 Apr 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : North Holland Publishing
Original Publication: Amsterdam, North-Holland.
مواضيع طبية MeSH: Adipogenesis*/drug effects , Estradiol*/pharmacology , Testosterone*/pharmacology , Sex Characteristics* , Adiponectin*/metabolism, Humans ; Male ; Female ; Triglycerides/metabolism ; Spheroids, Cellular/drug effects ; Spheroids, Cellular/metabolism ; Spheroids, Cellular/cytology ; Cells, Cultured ; PPAR gamma/metabolism ; PPAR gamma/genetics ; Adipocytes/drug effects ; Adipocytes/metabolism ; Adipocytes/cytology ; Stem Cells/metabolism ; Stem Cells/drug effects ; Stem Cells/cytology ; Adipose Tissue/cytology ; Adipose Tissue/metabolism ; Adipose Tissue/drug effects ; Lipolysis/drug effects
مستخلص: Using a three-dimensional (3-D) in vitro culture model, we report the dose dependent effect of 17β-estradiol and testosterone on the adipogenic differentiation and maturation of human adipose derived stem cells (hASCs) obtained from female and male patients. Considering sexual dimorphism, we expected male and female adipocytes to respond differently to the sex steroids. Both male and female hASC spheroids were exposed to 100 nM and 500 nM of 17β-estradiol and testosterone either at the beginning of the adipogenic maturation (Phase I) to discourage intracellular triglyceride accumulation or exposed after adipogenic maturation (Phase II) to reduce the intracellular triglyceride accumulation. The results show that 17β-estradiol leads to a dose dependent reduction in intracellular triglyceride accumulation in female hASC spheroids compared to the both untreated and testosterone-treated cells. Affirming our hypothesis, 17β-estradiol prevented intracellular triglyceride accumulation during Phase I, while it stimulated lipolysis during Phase II. PPAR-γ and adiponectin gene expression also reduced upon 17β-estradiol treatment in female cells. Interestingly, 17β-estradiol and testosterone had only a modest effect on the male hASC spheroids. Collectively, our findings suggest that 17β-estradiol can prevent fat accumulation in adipocytes during early and late stages of maturation in females.
Competing Interests: Declaration of competing interest Declarations of interest: none.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
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معلومات مُعتمدة: R01 EB020006 United States EB NIBIB NIH HHS
فهرسة مساهمة: Keywords: 3-D spheroids; Adiponectin; Female adipocyte; Male adipocyte; PPAR-γ; triglyceride
المشرفين على المادة: 4TI98Z838E (Estradiol)
3XMK78S47O (Testosterone)
0 (Adiponectin)
0 (Triglycerides)
0 (PPAR gamma)
تواريخ الأحداث: Date Created: 20240411 Date Completed: 20240601 Latest Revision: 20240603
رمز التحديث: 20240603
مُعرف محوري في PubMed: PMC11144096
DOI: 10.1016/j.mce.2024.112249
PMID: 38604550
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-8057
DOI:10.1016/j.mce.2024.112249