دورية أكاديمية
Sexual Dimorphism's impact on adipogenesis: A three-dimensional in vitro model treated with 17β-estradiol and testosterone.
العنوان: | Sexual Dimorphism's impact on adipogenesis: A three-dimensional in vitro model treated with 17β-estradiol and testosterone. |
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المؤلفون: | Pal P; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA., Maranon RO; Department of Physiology, Faculty of Medicine, Universidad Nacional de Tucuman, San Miguel de Tucumán, Argentina; National Council on Scientific and Technical Research (CONICET), Tucuman, Argentina., Rivera Gonzales OJ; Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA., Speed JS; Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA., Janorkar AV; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State St, Jackson, MS, 39216, USA. Electronic address: ajanorkar@umc.edu. |
المصدر: | Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 Aug 01; Vol. 589, pp. 112249. Date of Electronic Publication: 2024 Apr 09. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Limerick : North Holland Publishing Original Publication: Amsterdam, North-Holland. |
مواضيع طبية MeSH: | Adipogenesis*/drug effects , Estradiol*/pharmacology , Testosterone*/pharmacology , Sex Characteristics* , Adiponectin*/metabolism, Humans ; Male ; Female ; Triglycerides/metabolism ; Spheroids, Cellular/drug effects ; Spheroids, Cellular/metabolism ; Spheroids, Cellular/cytology ; Cells, Cultured ; PPAR gamma/metabolism ; PPAR gamma/genetics ; Adipocytes/drug effects ; Adipocytes/metabolism ; Adipocytes/cytology ; Stem Cells/metabolism ; Stem Cells/drug effects ; Stem Cells/cytology ; Adipose Tissue/cytology ; Adipose Tissue/metabolism ; Adipose Tissue/drug effects ; Lipolysis/drug effects |
مستخلص: | Using a three-dimensional (3-D) in vitro culture model, we report the dose dependent effect of 17β-estradiol and testosterone on the adipogenic differentiation and maturation of human adipose derived stem cells (hASCs) obtained from female and male patients. Considering sexual dimorphism, we expected male and female adipocytes to respond differently to the sex steroids. Both male and female hASC spheroids were exposed to 100 nM and 500 nM of 17β-estradiol and testosterone either at the beginning of the adipogenic maturation (Phase I) to discourage intracellular triglyceride accumulation or exposed after adipogenic maturation (Phase II) to reduce the intracellular triglyceride accumulation. The results show that 17β-estradiol leads to a dose dependent reduction in intracellular triglyceride accumulation in female hASC spheroids compared to the both untreated and testosterone-treated cells. Affirming our hypothesis, 17β-estradiol prevented intracellular triglyceride accumulation during Phase I, while it stimulated lipolysis during Phase II. PPAR-γ and adiponectin gene expression also reduced upon 17β-estradiol treatment in female cells. Interestingly, 17β-estradiol and testosterone had only a modest effect on the male hASC spheroids. Collectively, our findings suggest that 17β-estradiol can prevent fat accumulation in adipocytes during early and late stages of maturation in females. Competing Interests: Declaration of competing interest Declarations of interest: none. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
References: | Biol Sex Differ. 2017 Oct 24;8(1):33. (PMID: 29065927) Curr Opin Endocrinol Diabetes Obes. 2014 Oct;21(5):313-22. (PMID: 25105998) Am J Physiol Endocrinol Metab. 2010 Sep;299(3):E506-15. (PMID: 20570822) Steroids. 2013 Sep;78(9):920-6. (PMID: 23707571) Obes Res. 1993 Jul;1(4):245-51. (PMID: 16350577) Exp Cell Res. 2010 Aug 1;316(13):2087-98. (PMID: 20420826) Curr Opin Nephrol Hypertens. 2011 Mar;20(2):133-8. (PMID: 21178615) Aging Male. 2013 Jun;16(2):38-47. (PMID: 23581697) Am J Physiol Endocrinol Metab. 2001 Jun;280(6):E827-47. (PMID: 11350765) Diabetes Obes Metab. 2014 Mar;16(3):237-47. (PMID: 23964810) PLoS One. 2017 Sep 28;12(9):e0185403. (PMID: 28957363) J Steroid Biochem Mol Biol. 1990 Nov 30;37(4):553-8. (PMID: 2278839) Arch Intern Med. 1991 Oct;151(10):1925-33. (PMID: 1929679) Lancet. 1994 Jan 29;343(8892):269-70. (PMID: 7905101) Int J Mol Sci. 2019 Mar 08;20(5):. (PMID: 30857216) Mol Endocrinol. 2005 Apr;19(4):833-42. (PMID: 15695368) J Clin Endocrinol Metab. 1992 Oct;75(4):1092-8. (PMID: 1400877) Process Biochem. 2017 Aug;59:312-320. (PMID: 28966553) Adv Protein Chem Struct Biol. 2019;116:135-170. (PMID: 31036290) Int J Obes Relat Metab Disord. 1992 Dec;16(12):991-7. (PMID: 1335979) FEBS J. 2011 Feb;278(4):559-64. (PMID: 21182596) J Clin Endocrinol Metab. 1994 Apr;78(4):950-4. (PMID: 8157726) Diabetes Obes Metab. 2010 Nov;12(11):941-6. (PMID: 20880340) Diabetologia. 2006 Nov;49(11):2564-71. (PMID: 17001471) Climacteric. 1999 Jun;2(2):93-100. (PMID: 11910673) Aging Male. 2002 Jun;5(2):98-102. (PMID: 12198740) Nature. 2006 Dec 14;444(7121):881-7. (PMID: 17167477) Tissue Eng Part A. 2015 Jun;21(11-12):1837-47. (PMID: 25781458) Endocrinology. 2006 Jan;147(1):141-54. (PMID: 16210377) J Clin Endocrinol Metab. 2001 Oct;86(10):5045-51. (PMID: 11600583) Circulation. 2012 Apr 17;125(15):1905-19. (PMID: 22508840) Nutrients. 2021 Dec 20;13(12):. (PMID: 34960109) BMC Med. 2016 Oct 7;14(1):153. (PMID: 27716209) Circulation. 1994 Aug;90(2):786-91. (PMID: 8044949) Am J Physiol. 1994 Jun;266(6 Pt 1):E914-20. (PMID: 8023922) Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E304-19. (PMID: 19920214) Mol Biol Cell. 2002 Dec;13(12):4279-95. (PMID: 12475952) Endocrinology. 2014 Jul;155(7):2696-703. (PMID: 24735327) Int J Endocrinol. 2014;2014:527470. (PMID: 24688542) Curr Opin Endocrinol Diabetes Obes. 2010 Jun;17(3):224-32. (PMID: 20418719) J Biomed Sci. 2018 Dec 3;25(1):89. (PMID: 30509250) Diabetes Care. 1999 Sep;22(9):1401-7. (PMID: 10480500) Endocrinology. 2000 Feb;141(2):649-56. (PMID: 10650946) Front Endocrinol (Lausanne). 2022 Sep 21;13:921504. (PMID: 36213285) Int J Obes (Lond). 2012 Jun;36(6):843-54. (PMID: 21844879) Obesity (Silver Spring). 2006 Dec;14(12):2163-72. (PMID: 17189542) Medicine (Baltimore). 2018 Apr;97(17):e0482. (PMID: 29703008) JAMA Surg. 2014 Mar;149(3):275-87. (PMID: 24352617) J Biomed Mater Res A. 2014 Mar;102(3):852-61. (PMID: 23564487) Circulation. 1994 Apr;89(4):1501-10. (PMID: 8149515) J Clin Endocrinol Metab. 2004 Feb;89(2):718-26. (PMID: 14764787) Eur J Endocrinol. 2012 Oct;167(4):543-51. (PMID: 22872467) Biotechnol Bioeng. 2014 Jan;111(1):174-83. (PMID: 24038000) J Gerontol A Biol Sci Med Sci. 2003 Dec;58(12):M1103-10. (PMID: 14684707) J Cardiovasc Transl Res. 2009 Sep;2(3):321-7. (PMID: 20560019) Lipids Health Dis. 2017 Sep 25;16(1):182. (PMID: 28946914) J Clin Endocrinol Metab. 1993 May;76(5):1140-6. (PMID: 8496304) J Am Geriatr Soc. 2003 Jan;51(1):101-15; discussion 115. (PMID: 12534854) |
معلومات مُعتمدة: | R01 EB020006 United States EB NIBIB NIH HHS |
فهرسة مساهمة: | Keywords: 3-D spheroids; Adiponectin; Female adipocyte; Male adipocyte; PPAR-γ; triglyceride |
المشرفين على المادة: | 4TI98Z838E (Estradiol) 3XMK78S47O (Testosterone) 0 (Adiponectin) 0 (Triglycerides) 0 (PPAR gamma) |
تواريخ الأحداث: | Date Created: 20240411 Date Completed: 20240601 Latest Revision: 20240603 |
رمز التحديث: | 20240603 |
مُعرف محوري في PubMed: | PMC11144096 |
DOI: | 10.1016/j.mce.2024.112249 |
PMID: | 38604550 |
قاعدة البيانات: | MEDLINE |
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