An amino-rich polymer-coated magnetic nanomaterial for ultra-rapid separation of phosphorylated peptides in the serum of Parkinson's disease patients.

التفاصيل البيبلوغرافية
العنوان:	An amino-rich polymer-coated magnetic nanomaterial for ultra-rapid separation of phosphorylated peptides in the serum of Parkinson's disease patients.
المؤلفون:	Zhang X; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China., Wang B; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China., Luo Y; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China., Ding CF; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China. dingchuanfan@nbu.edu.cn., Yan Y; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China. yanyinghua@nbu.edu.cn.
المصدر:	Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Jun; Vol. 416 (14), pp. 3361-3371. Date of Electronic Publication: 2024 Apr 12.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Heidelberg : Springer-Verlag, 2002-
مواضيع طبية MeSH:	Parkinson Disease/blood , Phosphopeptides/blood , Polymers/chemistry , Nanostructures/chemistry, Humans ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
مستخلص:	The elucidation of disease pathogenesis can be achieved by analyzing the low-abundance phosphopeptides in organisms. Herein, we developed a novel and easy-to-prepare polymer-coated nanomaterial. By improving the hydrophilicity and spatial conformation of the material, we effectively enhanced the adsorption of phosphopeptides and demonstrated excellent enrichment properties. The material was able to successfully enrich the phosphopeptides in only 1 min. Meanwhile, the material has high selectivity (1:2000), good loading capacity (100 μg/mg), excellent sensitivity (0.5 fmol), and great acid and alkali resistance. In addition, the material was applied to real samples, and 70 phosphopeptides were enriched from the serum of Parkinson's disease (PD) patients and 67 phosphopeptides were enriched from the serum of normal controls. Sequences Logo showed that PD is probably associated with threonine, glutamate, serine, and glutamine. Finally, gene ontology (GO) analysis was performed on phosphopeptides enriched in PD patients' serum. The results showed that PD patients expressed abnormal expression of the cholesterol metabolic process and cell-matrix adhesion in the biological process (BP), endoplasmic reticulum and lipoprotein in the cellular component (CC), and heparin-binding, lipid-binding, and receptor-binding in the molecular function (MF) as compared with normal individuals. All the experiments indicate that the nanomaterials have great potential in proteomics studies. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
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معلومات مُعتمدة:	2023YFF0613402 National Key Research and Development Program of China; LY22B050008 Natural Science Foundation of Zhejiang Province
فهرسة مساهمة:	Keywords: Enrichment; MALDI-TOF MS; Magnetic nanomaterial; PD; Phosphopeptides
تواريخ الأحداث:	Date Created: 20240412 Date Completed: 20240520 Latest Revision: 20240520
رمز التحديث:	20240520
DOI:	10.1007/s00216-024-05287-9
PMID:	38607383
قاعدة البيانات:	MEDLINE

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تدمد:	1618-2650
DOI:	10.1007/s00216-024-05287-9