دورية أكاديمية
Detection of virus-specific T cells via ELISpot corroborates early diagnosis in human Borna disease virus 1 (BoDV-1) encephalitis.
| العنوان: | Detection of virus-specific T cells via ELISpot corroborates early diagnosis in human Borna disease virus 1 (BoDV-1) encephalitis. |
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| المؤلفون: | Bauswein M; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany. markus.bauswein@ukr.de., Eid E; Department of Neurology, University of Regensburg, Bezirksklinikum, Regensburg, Germany., Eidenschink L; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany., Schmidt B; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany., Gessner A; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany., Tappe D; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; German Consiliary Laboratory for Bornaviruses, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany., Cadar D; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany., Böhmer MM; Bavarian Health and Food Safety Authority, Munich, Germany.; Institute of Social Medicine and Health Systems Research, Otto-von-Guericke-University, Magdeburg, Germany., Jockel L; Department of Neurology, University of Regensburg, Bezirksklinikum, Regensburg, Germany., van Wickeren N; Department of Neurology, University of Regensburg, Bezirksklinikum, Regensburg, Germany., Garibashvili T; Department of Neurology, Klinikum Landshut, Landshut, Germany., Wiesinger I; Institute of Neuroradiology, University of Regensburg, Bezirksklinikum, Regensburg, Germany., Wendl C; Institute of Neuroradiology, University of Regensburg, Bezirksklinikum, Regensburg, Germany., Heckmann JG; Department of Neurology, Klinikum Landshut, Landshut, Germany., Angstwurm K; Department of Neurology, University of Regensburg, Bezirksklinikum, Regensburg, Germany., Freyer M; Department of Neurology, Klinikum Landshut, Landshut, Germany. |
| المصدر: | Infection [Infection] 2024 Apr 12. Date of Electronic Publication: 2024 Apr 12. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Heidelberg Country of Publication: Germany NLM ID: 0365307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0973 (Electronic) Linking ISSN: 03008126 NLM ISO Abbreviation: Infection Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Heidelberg : Springer Heidelberg Original Publication: München, Verlagsgesellschaft Otto Spatz. |
| مستخلص: | Background: Within endemic regions in southern and eastern Germany, Borna disease virus 1 (BoDV-1) causes rare zoonotic spill-over infections in humans, leading to encephalitis with a high case-fatality risk. So far, intra-vitam diagnosis has mainly been based on RT-qPCR from cerebrospinal fluid (CSF) and serology, both being associated with diagnostic challenges. Whilst low RNA copy numbers in CSF limit the sensitivity of RT-qPCR from this material, seroconversion often occurs late during the course of the disease. Case Presentation: Here, we report the new case of a 40 - 50 year-old patient in whom the detection of virus-specific T cells via ELISpot corroborated the diagnosis of BoDV-1 infection. The patient showed a typical course of the disease with prodromal symptoms like fever and headaches 2.5 weeks prior to hospital admission, required mechanical ventilation from day three after hospitalisation and remained in deep coma until death ten days after admission. Results: Infection was first detected by positive RT-qPCR from a CSF sample drawn four days after admission (viral load 890 copies/mL). A positive ELISpot result was obtained from peripheral blood collected on day seven, when virus-specific IgG antibodies were not detectable in serum, possibly due to previous immune adsorption for suspected autoimmune-mediated encephalitis. Conclusion: This case demonstrates that BoDV-1 ELISpot serves as additional diagnostic tool even in the first week after hospitalisation of patients with BoDV-1 encephalitis. (© 2024. The Author(s).) |
| References: | Schlottau K, Forth L, Angstwurm K, Höper D, Zecher D, Liesche F, et al. Fatal Encephalitic borna disease virus 1 in solid-organ transplant recipients. N Engl J Med. 2018;379:1377–9. https://doi.org/10.1056/NEJMc1803115 . (PMID: 10.1056/NEJMc180311530281984) Korn K, Coras R, Bobinger T, Herzog SM, Lücking H, Stöhr R, et al. Fatal encephalitis associated with borna disease virus 1. N Engl J Med. 2018. https://doi.org/10.1056/NEJMc1800724 . (PMID: 10.1056/NEJMc180072430281979) Pörtner K, Wilking H, Frank C, Böhmer MM, Stark K, Tappe D. Risk factors for Borna disease virus 1 encephalitis in Germany—case-control study. Emerg Microbes Infect. 2023;12: e2174778. https://doi.org/10.1080/22221751.2023.2174778 . (PMID: 10.1080/22221751.2023.2174778367483199980402) Dürrwald R, Kolodziejek J, Weissenböck H, Nowotny N. The bicolored white-toothed shrew Crocidura leucodon (HERMANN 1780) is an indigenous host of mammalian Borna disease virus. PLoS ONE. 2014;9: e93659. https://doi.org/10.1371/journal.pone.0093659 . (PMID: 10.1371/journal.pone.0093659246996363974811) Grosse L, Lieftüchter V, Vollmuth Y, Hoffmann F, Olivieri M, Reiter K, et al. First detected geographical cluster of BoDV-1 encephalitis from same small village in two children: therapeutic considerations and epidemiological implications. Infection. 2023;51:1383–98. https://doi.org/10.1007/s15010-023-01998-w . (PMID: 10.1007/s15010-023-01998-w368210249947883) Meyer T, Tappe D, Hasan D, Rust M, Schulz JB, Schiefer J, Tauber SC. „Borna disease virus 1“(BoDV-1)-Enzephalitis eines 18-Jährigen außerhalb des bisher bekannten Endemiegebietes. DGNeurologie. 2022;5:300–4. https://doi.org/10.1007/s42451-022-00440-z . (PMID: 10.1007/s42451-022-00440-z9125534) Neumann B, Hierl A, Wunderlich S, Meier H, Bauer C, Gerner ST, et al. Cerebrospinal fluid in borna disease virus 1 (BoDV-1) encephalitis. J Neurol Sci. 2023. https://doi.org/10.1016/j.jns.2023.120568 . (PMID: 10.1016/j.jns.2023.12056836716549) Finck T, Liesche-Starnecker F, Probst M, Bette S, Ruf V, Wendl C, et al. Bornavirus encephalitis shows a characteristic magnetic resonance phenotype in humans. Ann Neurol. 2020;88:723–35. https://doi.org/10.1002/ana.25873 . (PMID: 10.1002/ana.2587332794235) Neumann B, Angstwurm K, Linker RA, Knoll G, Eidenschink L, Rubbenstroth D, et al. Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal borna disease virus 1 infections. Cell Rep Med. 2022. https://doi.org/10.1016/j.xcrm.2021.100499 . (PMID: 10.1016/j.xcrm.2021.100499351065118784767) Eisermann P, Rubbenstroth D, Cadar D, Thomé-Bolduan C, Eggert P, Schlaphof A, et al. Active case finding of current bornavirus infections in human encephalitis cases of unknown etiology, Germany, 2018–2020. Emerg Infect Dis. 2021;27:1371–9. https://doi.org/10.3201/eid2705.204490 . (PMID: 10.3201/eid2705.204490339001678084505) Hallensleben W, Schwemmle M, Hausmann J, Stitz L, Volk B, Pagenstecher A, Staeheli P. Borna disease virus-induced neurological disorder in mice: infection of neonates results in immunopathology. J Virol. 1998;72:4379–86. https://doi.org/10.1128/JVI.72.5.4379-4386.1998 . (PMID: 10.1128/JVI.72.5.4379-4386.19989557728109668) Eidenschink L, Knoll G, Tappe D, Offner R, Drasch T, Ehrl Y, et al. IFN-γ-Based ELISpot as a new tool to detect human infections with borna disease virus 1 (BoDV-1): a pilot study. Viruses. 2023. https://doi.org/10.3390/v15010194 . (PMID: 10.3390/v15010194366802349864614) Wenzel JJ, Walch H, Bollwein M, Niller HH, Ankenbauer W, Mauritz R, et al. Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus. Clin Chem. 2009;55:2218–22. https://doi.org/10.1373/clinchem.2009.136192 . (PMID: 10.1373/clinchem.2009.13619219797710) Dreier J, Störmer M, Kleesiek K. Use of bacteriophage MS2 as an internal control in viral reverse transcription-PCR assays. J Clin Microbiol. 2005;43:4551–7. https://doi.org/10.1128/JCM.43.9.4551-4557.2005 . (PMID: 10.1128/JCM.43.9.4551-4557.2005161451061234060) Bauswein M, Eidenschink L, Knoll G, Neumann B, Angstwurm K, Zoubaa S, et al. Human infections with borna disease virus 1 (BoDV-1) primarily lead to severe encephalitis: further evidence from the seroepidemiological BoSOT study in an endemic region in southern Germany. Viruses. 2023. https://doi.org/10.3390/v15010188 . (PMID: 10.3390/v15010188366802349864614) Tappe D, Frank C, Offergeld R, Wagner-Wiening C, Stark K, Rubbenstroth D, et al. Low prevalence of borna disease virus 1 (BoDV-1) IgG antibodies in humans from areas endemic for animal Borna disease of Southern Germany. Sci Rep. 2019. https://doi.org/10.1038/s41598-019-56839-4 . (PMID: 10.1038/s41598-019-56839-4318829426934520) Allartz P, Hotop S-K, Muntau B, Schlaphof A, Thomé-Bolduan C, Gabriel M, et al. Detection of bornavirus-reactive antibodies and BoDV-1 RNA only in encephalitis patients from virus endemic areas: a comparative serological and molecular sensitivity, specificity, predictive value, and disease duration correlation study. Infection. 2023. https://doi.org/10.1007/s15010-023-02048-1 . (PMID: 10.1007/s15010-023-02048-13725381610228883) Coras R, Korn K, Kuerten S, Huttner HB, Ensser A. Severe bornavirus-encephalitis presenting as guillain-barré-syndrome. Acta Neuropathol. 2019;137:1017–9. https://doi.org/10.1007/s00401-019-02005-z . (PMID: 10.1007/s00401-019-02005-z30953131) Planz O, Stitz L. Borna disease virus nucleoprotein (p40) is a major target for CD8(+)-T-cell-mediated immune response. J Virol. 1999;73:1715–8. https://doi.org/10.1128/JVI.73.2.1715-1718.1999 . (PMID: 10.1128/JVI.73.2.1715-1718.19999882386104005) |
| معلومات مُعتمدة: | ReForM-A program University Hospital Regensburg, Germany; 01KI2002 German Federal Ministry of Education and Research; 01KI2005F German Federal Ministry of Education and Research; Bornavirus - Focal Point Bavaria 2.0 Bavarian State Ministry of Health and Care; Bornavirus - Focal Point Bavaria 2.0 Bavarian State Ministry of Health and Care; PREPMedVet no. 13N15449 German Ministry of Education and Research |
| فهرسة مساهمة: | Keywords: Borna disease virus 1 (BoDV-1); ELISpot; Encephalitis; Indirect immune fluorescence assay (iIFA); Zoonosis |
| تواريخ الأحداث: | Date Created: 20240412 Latest Revision: 20240412 |
| رمز التحديث: | 20240412 |
| DOI: | 10.1007/s15010-024-02246-5 |
| PMID: | 38607591 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1439-0973 |
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| DOI: | 10.1007/s15010-024-02246-5 |