دورية أكاديمية
A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation.
| العنوان: | A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation. |
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| المؤلفون: | Ye Y; College of Life Science, Capital Normal University, Beijing, 100048, China., Liu H; College of Life Science, Capital Normal University, Beijing, 100048, China., Wang Z; College of Life Science, Capital Normal University, Beijing, 100048, China., Qi Q; Beijing Chaoyang Foreign Language School, Beijing, 100012, China., Du J; College of Life Science, Capital Normal University, Beijing, 100048, China., Tian S; College of Life Science, Capital Normal University, Beijing, 100048, China. cnu_tianshen@sina.com. |
| المصدر: | Biotechnology letters [Biotechnol Lett] 2024 Aug; Vol. 46 (4), pp. 531-543. Date of Electronic Publication: 2024 Apr 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : Dordrecht : Kluwer Academic Publishers Original Publication: [Kew, Eng., Science and Technology Letters] |
| مواضيع طبية MeSH: | Ethanol*/metabolism , Lignin*/metabolism , Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae*/enzymology , Fermentation* , Cellulosomes*/metabolism , Cellulosomes*/genetics, Cellulose/metabolism ; Laccase/metabolism ; Laccase/genetics |
| مستخلص: | Biofuel production from lignocellulose feedstocks is sustainable and environmentally friendly. However, the lignocellulosic pretreatment could produce fermentation inhibitors causing multiple stresses and low yield. Therefore, the engineering construction of highly resistant microorganisms is greatly significant. In this study, a composite functional chimeric cellulosome equipped with laccase, versatile peroxidase, and lytic polysaccharide monooxygenase was riveted on the surface of Saccharomyces cerevisiae to construct a novel yeast strain YI/LVP for synergistic lignin degradation and cellulosic ethanol production. The assembly of cellulosome was assayed by immunofluorescence microscopy and flow cytometry. During the whole process of fermentation, the maximum ethanol concentration and cellulose conversion of engineering strain YI/LVP reached 8.68 g/L and 83.41%, respectively. The results proved the availability of artificial chimeric cellulosome containing lignin-degradation enzymes for cellulosic ethanol production. The purpose of the study was to improve the inhibitor tolerance and fermentation performance of S. cerevisiae through the construction and optimization of a synergistic lignin-degrading enzyme system based on cellulosome. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.) |
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| معلومات مُعتمدة: | 31971202 National Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: Saccharomyces cerevisiae; Cellulosic ethanol; Cellulosome; Lignin degradation; Separate hydrolysis and fermentation; Surface display |
| المشرفين على المادة: | 3K9958V90M (Ethanol) 9005-53-2 (Lignin) 9004-34-6 (Cellulose) EC 1.10.3.2 (Laccase) |
| تواريخ الأحداث: | Date Created: 20240412 Date Completed: 20240702 Latest Revision: 20240702 |
| رمز التحديث: | 20240702 |
| DOI: | 10.1007/s10529-024-03485-0 |
| PMID: | 38607604 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1573-6776 |
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| DOI: | 10.1007/s10529-024-03485-0 |