دورية أكاديمية
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A papain-based colorimetric catalytic sensing system for immunoassay detection of carcinoembryonic antigen.

التفاصيل البيبلوغرافية
العنوان: A papain-based colorimetric catalytic sensing system for immunoassay detection of carcinoembryonic antigen.
المؤلفون: Li X; Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Province, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, People's Republic of China., Lu X; Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Province, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, People's Republic of China., Zhang L; Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Province, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, People's Republic of China., Cai Z; Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Province, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, People's Republic of China. Electronic address: czx1816@mnnu.edu.cn., Tang D; Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China., Lai W; Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Province, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, People's Republic of China. Electronic address: wenqiang.lai@mnnu.edu.cn.
المصدر: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Jul 05; Vol. 315, pp. 124269. Date of Electronic Publication: 2024 Apr 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 9602533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3557 (Electronic) Linking ISSN: 13861425 NLM ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Subsets: MEDLINE
أسماء مطبوعة: Publication: : Amsterdam : Elsevier
Original Publication: [Kidlington, Oxford, U.K. ; Tarrytown, NY] : Pergamon, c1994-
مواضيع طبية MeSH: Carcinoembryonic Antigen*/analysis , Colorimetry*/methods , Papain*/metabolism , Limit of Detection*, Immunoassay/methods ; Humans ; Glucose Oxidase/chemistry ; Glucose Oxidase/metabolism ; Hydrogen Peroxide/chemistry ; Catalysis ; Benzidines/chemistry ; Biosensing Techniques/methods ; Reproducibility of Results
مستخلص: A colorimetric immunoassay was built for determination of carcinoembryonic antigen (CEA) based on papain-based colorimetric catalytic sensing system through the use of glucose oxidase (GOx). In the presence of GOx, glucose was catalytically oxidized to produce H 2 O 2 . Through the assistance of papain (as a peroxide mimetic enzyme), the signal came from the oxidative color development of 3,3',5,5'-tetramethylbenzidine (TMB, from colorless to blue) catalyzed by the generated H 2 O 2 . Herein, a sandwich-type immunoassay was built based on GOx as labels. As the concentration of CEA increased, more GOx-labeled antibodies specifically associate with target, which leaded to more H 2 O 2 generation. Immediately following this, more TMB were oxidized with the addition of papain. Accordingly, the absorbance increased further. As a result, the concentration of CEA is positively correlated with the change in absorbance of the solution. Under optimal conditions, the CEA concentration was linear in the range of 0.05-20.0 ng/mL, and the limit of detection (LOD) reached 37 pg/mL. The papain-based colorimetric immunoassay also exhibited satisfactory repeatability, stability, and selectivity.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
المشرفين على المادة: 0 (Carcinoembryonic Antigen)
EC 3.4.22.2 (Papain)
EC 1.1.3.4 (Glucose Oxidase)
BBX060AN9V (Hydrogen Peroxide)
0 (Benzidines)
3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine)
تواريخ الأحداث: Date Created: 20240412 Date Completed: 20240504 Latest Revision: 20240504
رمز التحديث: 20240505
DOI: 10.1016/j.saa.2024.124269
PMID: 38608561
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3557
DOI:10.1016/j.saa.2024.124269