دورية أكاديمية
Cultivation of phosphate-accumulating biofilm: Study of the effects of acyl-homoserine lactones (AHLs) and cyclic dimeric guanosine monophosphate (c-di-GMP) on the formation of biofilm and the enhancement of phosphate metabolism capacity.
| العنوان: | Cultivation of phosphate-accumulating biofilm: Study of the effects of acyl-homoserine lactones (AHLs) and cyclic dimeric guanosine monophosphate (c-di-GMP) on the formation of biofilm and the enhancement of phosphate metabolism capacity. |
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| المؤلفون: | Zhao Y; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China., Zhang J; Suzhou Drainage Company Limited, Suzhou 215009, China., Ni M; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; Jiangsu Key Laboratory of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China., Pan Y; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China. Electronic address: panyang@mail.usts.edu.cn., Li L; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China., Ding Y; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Jun 10; Vol. 928, pp. 172408. Date of Electronic Publication: 2024 Apr 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Cyclic GMP*/*analogs & derivatives, Biofilms* , Acyl-Butyrolactones*/metabolism , Phosphates*/metabolism , Cyclic GMP*/metabolism , Waste Disposal, Fluid*/methods, Bioreactors/microbiology ; Sewage/microbiology |
| مستخلص: | This study investigated the mechanisms of microbial growth and metabolism during biofilm cultivation in the biofilm sequencing batch reactor (BSBR) process for phosphate (P) enrichment. The results showed that the sludge discharge was key to biofilm growth, as it terminated the competition for carbon (C) source between the nascent biofilm and the activated sludge. For the tested reactor, after the sludge discharge on 18 d, P metabolism and C source utilization improved significantly, and the biofilm grew rapidly. The P concentration of the recovery liquid reached up to 157.08 mg/L, which was sufficient for further P recovery via mineralization. Meta-omics methods were used to analyze metabolic pathways and functional genes in microbial growth during biofilm cultivation. It appeared that the sludge discharge activated the key genes of P metabolism and inhibited the key genes of C metabolism, which strengthened the polyphosphate-accumulating metabolism (PAM) as a result. The sludge discharge not only changed the types of polyphosphate-accumulating organisms (PAOs) but also promoted the growth of dominant PAOs. Before the sludge discharge, the necessary metabolic abilities that were spread among different microorganisms gradually concentrated into a small number of PAOs, and after the sludge discharge, they further concentrated into Candidatus_Contendobacter (P3) and Candidatus_Accumulibacter (P17). The messenger molecule C-di-GMP, produced mostly by P3 and P17, facilitated P enrichment by regulating cellular P and C metabolism. The glycogen-accumulating organism (GAO) Candidatus_Competibacter secreted N-Acyl homoserine lactones (AHLs), which stimulated the secretion of protein in extracellular polymeric substances (EPS), thus promoting the adhesion of microorganisms to biofilm and improving P metabolism via EPS-based P adsorption. Under the combined action of the dominant GAOs and PAOs, AHLs and C-di-GMP mediated QS to promote biofilm development and P enrichment. The research provides theoretical support for the cultivation of biofilm and its wider application. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: AHLs; Biofilm cultivation; Biofilm sequencing batch reactor; C-di-GMP; Phosphate recovery; Quorum sensing |
| المشرفين على المادة: | 0 (Acyl-Butyrolactones) 0 (Phosphates) H2D2X058MU (Cyclic GMP) 61093-23-0 (bis(3',5')-cyclic diguanylic acid) 0 (Sewage) |
| تواريخ الأحداث: | Date Created: 20240412 Date Completed: 20240430 Latest Revision: 20240430 |
| رمز التحديث: | 20240501 |
| DOI: | 10.1016/j.scitotenv.2024.172408 |
| PMID: | 38608880 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2024.172408 |