دورية أكاديمية
Feline leishmaniasis in an animal shelter in northeastern Brazil: Clinical aspects, coinfections, molecular detection, and serological study of a new recombinant protein.
| العنوان: | Feline leishmaniasis in an animal shelter in northeastern Brazil: Clinical aspects, coinfections, molecular detection, and serological study of a new recombinant protein. |
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| المؤلفون: | Nascimento LFJ; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Amado-Gomes AC; Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Dantas-Torres F; Aggeu Magalhães Institute, Fundação Oswaldo Cruz (Fiocruz), Recife, PE, Brazil., Santos FLN; Integrated Translational Program in Chagas Disease from Fiocruz (Fio-Chagas), Oswaldo Cruz Foundation (Fiocruz-RJ), Rio de Janeiro, Brazil; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil., Neres WS; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, SE, Brazil., Filho PES; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Santos MT; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Silva JRS; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Resende CF; Department of Veterinary Preventive Medicine, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Dos Reis JKP; Department of Veterinary Preventive Medicine, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Jain S; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Graduate Program in Industrial Biotechnology, Tiradentes University, SE, Brazil., Cunha JLR; York Biomedical Research Institute and Department of Biology, University of York, York, United Kingdom., Fujiwara RT; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: rtfujiwara@gmail.com., Dolabella SS; Graduate Program in Parasite Biology, Federal University of Sergipe, São Cristóvão, SE, Brazil. Electronic address: dolabellaufs@gmail.com. |
| المصدر: | Research in veterinary science [Res Vet Sci] 2024 Jun; Vol. 172, pp. 105256. Date of Electronic Publication: 2024 Apr 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : British Veterinary Association Original Publication: Oxford. |
| مواضيع طبية MeSH: | Cat Diseases*/diagnosis , Cat Diseases*/parasitology , Cat Diseases*/virology , Cat Diseases*/blood , Cat Diseases*/epidemiology , Enzyme-Linked Immunosorbent Assay*/veterinary , Immunodeficiency Virus, Feline*/isolation & purification , Coinfection*/veterinary , Coinfection*/parasitology , Coinfection*/epidemiology , Coinfection*/virology , Leishmania infantum*/isolation & purification , Leukemia Virus, Feline*/genetics , Leukemia Virus, Feline*/immunology , Recombinant Proteins*, Cats ; Animals ; Brazil/epidemiology ; Male ; Female ; Toxoplasma ; Antibodies, Protozoan/blood ; Leishmaniasis, Visceral/veterinary ; Leishmaniasis, Visceral/diagnosis ; Leishmaniasis, Visceral/epidemiology ; Leishmaniasis, Visceral/blood ; Polymerase Chain Reaction/veterinary ; Toxoplasmosis, Animal/diagnosis ; Toxoplasmosis, Animal/epidemiology ; Toxoplasmosis, Animal/blood |
| مستخلص: | Infection and clinical cases of leishmaniasis caused by Leishmania infantum in cats have been increasingly reported in several countries, including Brazil. In this study, we used an enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic test (ICT) based on a recombinant antigen (rKDDR-plus) to detect anti-Leishmania antibodies in cats from an animal shelter in northeastern Brazil. We compared the results with an ELISA using L. infantum crude antigen (ELISA-CA). We also investigated the presence of Leishmania DNA in blood or ocular conjunctival samples as well as the association between Leishmania PCR positivity and serological positivity to feline immunodeficiency virus (FIV), feline leukemia virus (FeLV) and Toxoplasma gondii. Concerning serological assays, a higher positivity was detected using the ICT-rKDDR-plus (7.5%; 7/93) as compared to ELISA-rKDDR-plus (5.4%; 5/93) and ELISA-CA (4.3%; 4/93). Upon PCR testing, 52.7% (49/93) of the ocular conjunctival swabs and 48.3% (44/91) of the blood samples were positive. Together, PCR and serological testing revealed overall positivities of 73.1% (68/93) and 12.9% (12/93), respectively. Among PCR-positive samples, 45.5% (31/68) showed co-infection with FIV, 17.6% (12/68) with FeLV, and 82.3% (56/68) with T. gondii. More than half of the PCR-positive cats showed at least one clinical sign suggestive of leishmaniasis (58.8%; 40/68) and dermatological signs were the most frequent ones (45.5%; 31/68). Both tests employing the recombinant antigen rKDDR-plus (i.e., ICT-rKDDR-plus and ELISA-rKDDR-plus) detected more positive cats than the ELISA-CA but presented low overall accuracy. PCR testing using either blood or ocular conjunctival samples detected much more positive cats than serological tests. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Clinical signs; Feline leishmaniasis; Immunological diagnosis; Leishmania coinfections; rKDDR-plus |
| المشرفين على المادة: | 0 (Recombinant Proteins) 0 (Antibodies, Protozoan) |
| تواريخ الأحداث: | Date Created: 20240413 Date Completed: 20240506 Latest Revision: 20240506 |
| رمز التحديث: | 20240507 |
| DOI: | 10.1016/j.rvsc.2024.105256 |
| PMID: | 38613921 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2661 |
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| DOI: | 10.1016/j.rvsc.2024.105256 |