دورية أكاديمية
Exploring the Correlation between Interleukin-17A Promoter Polymorphism at its -197 G/A and Rheumatoid Arthritis: Impact on Disease Severity and Activity.
| العنوان: | Exploring the Correlation between Interleukin-17A Promoter Polymorphism at its -197 G/A and Rheumatoid Arthritis: Impact on Disease Severity and Activity. |
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| المؤلفون: | Fahmy EM; Department of Medical Microbiology and Immunology, Faculty of Medicine, Helwan University, Cairo, Egypt., Nageeb HM; Department of Medical Microbiology and Immunology, Faculty of Medicine, Aswan University, Aswan, Egypt., Sadek A; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt., El Nouby FH; Department of Rheumatology, Faculty of Medicine, Assiut University, Assiut, Egypt., Aglan LI; Department of Rheumatology, Faculty of Medicine, Aswan University, Aswan, Egypt., Amin MM; Department of Medical Microbiology and Immunology, Faculty of Medicine, Aswan University, Aswan, Egypt. |
| المصدر: | The Egyptian journal of immunology [Egypt J Immunol] 2024 Apr; Vol. 31 (2), pp. 1-9. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Modern Commercial Press Country of Publication: Egypt NLM ID: 9816016 Publication Model: Print Cited Medium: Print ISSN: 1110-4902 (Print) NLM ISO Abbreviation: Egypt J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cairo : Modern Commercial Press, |
| مواضيع طبية MeSH: | Arthritis, Rheumatoid*/genetics , Interleukin-17*/blood , Interleukin-17*/chemistry , Interleukin-17*/genetics, Humans ; Anti-Citrullinated Protein Antibodies ; C-Reactive Protein/chemistry ; Case-Control Studies ; Patient Acuity ; Polymorphism, Genetic ; Rheumatoid Factor ; Promoter Regions, Genetic |
| مستخلص: | T helper 17 (Th17) cells have been reported to be the most powerful factor in autoimmune disorder pathogenesis, which points to the Th17 master cytokine, interleukin (IL)-17A, as the crucial mediator. We aimed to determine the impact of IL-17A polymorphism in the -197 G/A promoter region on level of IL-17 and intensity of rheumatoid arthritis (RA) disease symptoms. This case-control study was conducted at the Department of Clinical Rheumatology of Aswan university Hospital and included 35 people suffering RA and 30 volunteer controls, matched for age and sex. Rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP) antibodies, erythrocyte sedimentation rate (ESR), serum IL-17, and C-reactive protein (CRP) were measured in the RA patient group. Restriction fragment length polymorphism (RFLP) was conducted by polymerase chain reaction (PCR) amplicon obtained by IL-17A -197 G /A primers. Of the 35 RA patients, RF was positive in 33 (94.29%) and anti-CCP antibodies in 25 (71.43%), CRP in 31 (88.57%). Of the 35 RA patients, 5 (14.29%) patients carried the G/G genotype, 18 (51.43%) G/A and 12 (34.29%) A/A. IL-17 serum level was significantly greater in the more active RA (DAS28 >5.1) group than the less active (DAS28 ≤5.1) group. Of the RA patients carrying wild type G/G genotype, 60% had more active disease (DAS 28> 5.1), as compared to those with lower activity (DAS 28 ≤5.1), 40% carried the wild type G/G genotype. In conclusion, the study findings imply that IL-17A gene polymorphism is connected to RA clinical severity rather than with RA susceptibility. (Copyright© by the Egyptian Association of Immunologists.) |
| المشرفين على المادة: | 0 (Anti-Citrullinated Protein Antibodies) 9007-41-4 (C-Reactive Protein) 0 (Interleukin-17) 9009-79-4 (Rheumatoid Factor) |
| تواريخ الأحداث: | Date Created: 20240414 Date Completed: 20240415 Latest Revision: 20240419 |
| رمز التحديث: | 20240420 |
| PMID: | 38615199 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1110-4902 |
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