دورية أكاديمية
Characterization of immortalized ovarian epithelial cells with BRCA1/2 mutation.
| العنوان: | Characterization of immortalized ovarian epithelial cells with BRCA1/2 mutation. |
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| المؤلفون: | Komatsu H; Department of Obstetrics and Gynecology, Tottori University School of Medicine, 36-1 Nishicho, Yonago, Tottori, 683-8504, Japan. komatsu.h.med@gmail.com., Okawa M; Department of Obstetrics and Gynecology, Tottori University School of Medicine, 36-1 Nishicho, Yonago, Tottori, 683-8504, Japan., Kazuki Y; Department of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, Tottori, Japan., Kazuki K; Department of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, Tottori, Japan., Hichiwa G; Department of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, Tottori, Japan., Shimoya K; Department of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, Tottori, Japan., Sato S; Department of Obstetrics and Gynecology, Tottori University School of Medicine, 36-1 Nishicho, Yonago, Tottori, 683-8504, Japan., Taniguchi F; Department of Obstetrics and Gynecology, Tottori University School of Medicine, 36-1 Nishicho, Yonago, Tottori, 683-8504, Japan., Oshimura M; Transchromosomics Inc, Tottori, Japan., Harada T; Tottori University, Tottori, Japan. |
| المصدر: | Human cell [Hum Cell] 2024 Jul; Vol. 37 (4), pp. 986-996. Date of Electronic Publication: 2024 Apr 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Japan NLM ID: 8912329 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1749-0774 (Electronic) Linking ISSN: 09147470 NLM ISO Abbreviation: Hum Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Tokyo : Springer Original Publication: Tōkyō-to : Hito Saibō Kenkyūkai : Kanishobo, Shōwa 63-nen [1988]- |
| مواضيع طبية MeSH: | Epithelial Cells*/metabolism , Ovary*/cytology , Ovary*/metabolism , BRCA1 Protein*/genetics , BRCA2 Protein*/genetics, Humans ; Female ; Ovarian Neoplasms/genetics ; Ovarian Neoplasms/pathology ; Epithelial Cell Adhesion Molecule/genetics ; Epithelial Cell Adhesion Molecule/metabolism ; Gene Expression/genetics ; Mutation/genetics ; Cell Line, Transformed ; Germ-Line Mutation/genetics ; Telomerase/genetics ; Genes, BRCA1 ; Carcinogenesis/genetics |
| مستخلص: | We aimed to elucidate the mechanism underlying carcinogenesis by comparing normal and BRCA1/2-mutated ovarian epithelial cells established via Sendai virus-based immortalization. Ovarian epithelial cells (normal epithelium: Ovn; with germline BRCA1 mutation: OvBRCA1; with germline BRCA2 mutation: OvBRCA2) were infected with Sendai virus vectors carrying three immortalization genes (Bmi-1, hTERT, and SV40T). The immunoreactivity to anti-epithelial cellular adhesion molecule (EpCAM) antibodies in each cell line and cells after 25 passages was confirmed using flow cytometry. Chromosomes were identified and karyotyped to detect numerical and structural abnormalities. Total RNA extracted from the cells was subjected to human transcriptome sequencing. Highly expressed genes in each cell line were confirmed using real-time polymerase chain reaction. Immortalization techniques allowed 25 or more passages of Ovn, OvBRCA1, and OvBRCA2 cells. No anti-EpCAM antibody reactions were observed in primary cultures or after long-term passages of each cell line. Structural abnormalities in the chromosomes were observed in each cell line; however, the abnormal chromosomes were successfully separated from the normal structures via cloning. Only normal cells from each cell line were cloned. MMP1, CCL2, and PAPPA were more predominantly expressed in OvBRCA1 and OvBRCA2 cells than in Ovn cells. Immortalized ovarian cells derived from patients with germline BRCA1 or BRCA2 mutations showed substantially higher MMP1 expression than normal ovarian cells. However, the findings need to be validated in the future. (© 2024. The Author(s) under exclusive licence to Japan Human Cell Society.) |
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| معلومات مُعتمدة: | 21K16770 Japan Society for the Promotion of Science |
| فهرسة مساهمة: | Keywords: BRCA1/2; Carcinogenesis; Ovarian epithelial cancer; Sendai virus |
| المشرفين على المادة: | 0 (BRCA1 Protein) 0 (BRCA2 Protein) 0 (BRCA1 protein, human) 0 (BRCA2 protein, human) 0 (Epithelial Cell Adhesion Molecule) EC 2.7.7.49 (Telomerase) |
| تواريخ الأحداث: | Date Created: 20240414 Date Completed: 20240623 Latest Revision: 20240711 |
| رمز التحديث: | 20240711 |
| DOI: | 10.1007/s13577-024-01064-z |
| PMID: | 38615309 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1749-0774 |
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| DOI: | 10.1007/s13577-024-01064-z |