دورية أكاديمية
Peritoneal B1 and B2 cells respond differently to LPS and IL-21 stimulation.
| العنوان: | Peritoneal B1 and B2 cells respond differently to LPS and IL-21 stimulation. |
|---|---|
| المؤلفون: | Li D; Department of Pathogenic Microbiology and Immunology, School of Basic Medical, Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China., Ma Y; The Clinical Laboratory, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China., Miao Y; Blood Transfusion Department, Xi'an No. 3 Hospital, the Affiliated Hospital of Northwest University, China., Liu S; The Clinical Laboratory, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China., Bi Y; Department of Pathogenic Microbiology and Immunology, School of Basic Medical, Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China., Ji Y; Department of Pathogenic Microbiology and Immunology, School of Basic Medical, Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China., Wu Q; Department of Thoracic Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China., Zhou C; Department of Breast Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Electronic address: zhoucanz2005@xjtufh.edu.cn., Ma Y; Department of Pathogenic Microbiology and Immunology, School of Basic Medical, Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. Electronic address: mayunfeng@xjtu.edu.cn. |
| المصدر: | Molecular immunology [Mol Immunol] 2024 Jun; Vol. 170, pp. 46-56. Date of Electronic Publication: 2024 Apr 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 7905289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9142 (Electronic) Linking ISSN: 01615890 NLM ISO Abbreviation: Mol Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, Elmsford, N. Y., Pergamon Press. |
| مواضيع طبية MeSH: | Apoptosis*/drug effects , Apoptosis*/immunology , B-Lymphocyte Subsets*/drug effects , B-Lymphocyte Subsets*/immunology , Interleukin-10*/immunology , Interleukin-10*/metabolism , Interleukins*/immunology , Interleukins*/pharmacology , Lipopolysaccharides*/pharmacology , Lipopolysaccharides*/immunology , Peritoneum*/immunology , Peritoneum*/cytology, Animals ; Mice ; Antigens, CD19/immunology ; Antigens, CD19/metabolism ; B-Lymphocytes/drug effects ; B-Lymphocytes/immunology ; bcl-X Protein/metabolism ; bcl-X Protein/immunology ; CD11b Antigen/metabolism ; CD11b Antigen/immunology ; Mice, Inbred C57BL ; Phosphorylation/drug effects ; STAT3 Transcription Factor/metabolism ; STAT3 Transcription Factor/immunology |
| مستخلص: | Peritoneal B cells can be divided into B1 cells (CD11b + CD19 + ) and B2 cells (CD11b - CD19 + ) based on CD11b expression. B1 cells play a crucial role in the innate immune response by producing natural antibodies and cytokines. B2 cells share similar traits with B1 cells, influenced by the peritoneal environment. However, the response of both B1 and B2 cells to the same stimuli in the peritoneum remains uncertain. We isolated peritoneal B1 and B2 cells from mice and assessed differences in Interleukin-10(IL-10) secretion, apoptosis, and surface molecule expression following exposure to LPS and Interleukin-21(IL-21). Our findings indicate that B1 cells are potent IL-10 producers, possessing surface molecules with an IgM hi CD43 + CD21 low profile, and exhibit a propensity for apoptosis in vitro. Conversely, B2 cells exhibit lower IL-10 production and surface markers characterized as IgM low CD43 - CD21 hi , indicative of some resistance to apoptosis. LPS stimulates MAPK phosphorylation in B1 and B2 cells, causing IL-10 production. Furthermore, LPS inhibits peritoneal B2 cell apoptosis by enhancing Bcl-xL expression. Conversely, IL-21 has no impact on IL-10 production in these cells. Nevertheless, impeding STAT3 phosphorylation permits IL-21 to increase IL-10 production in peritoneal B cells. Moreover, IL-21 significantly raises apoptosis levels in these cells, a process independent of STAT3 phosphorylation and possibly linked to reduced Bcl-xL expression. This study elucidates the distinct functional and response profiles of B1 and B2 cells in the peritoneum to stimuli like LPS and IL-21, highlighting their differential roles in immunological responses and B cell diversity. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Interleukin-10; Interleukin-21; Lipopolysaccharide; Peritoneal B cells |
| المشرفين على المادة: | 0 (Antigens, CD19) 0 (bcl-X Protein) 0 (CD11b Antigen) 130068-27-8 (Interleukin-10) MKM3CA6LT1 (interleukin-21) 0 (Interleukins) 0 (Lipopolysaccharides) 0 (STAT3 Transcription Factor) |
| تواريخ الأحداث: | Date Created: 20240414 Date Completed: 20240511 Latest Revision: 20240628 |
| رمز التحديث: | 20240629 |
| DOI: | 10.1016/j.molimm.2024.04.007 |
| PMID: | 38615627 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-9142 |
|---|---|
| DOI: | 10.1016/j.molimm.2024.04.007 |