دورية أكاديمية
Overexpression of Rhodiola crenulata glutathione peroxidase 5 increases cold tolerance and enhances the pharmaceutical value of the hairy roots.
| العنوان: | Overexpression of Rhodiola crenulata glutathione peroxidase 5 increases cold tolerance and enhances the pharmaceutical value of the hairy roots. |
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| المؤلفون: | Hou K; Pu'er People's Hospital, Yunnan, China; Tianjin Chest Hospital, Tianjin, China; Chest Hospital, Tianjin University, Tianjin, China; Tianjin Medical University, Tianjin, China., Cao L; Tianjin Chest Hospital, Tianjin, China; Chest Hospital, Tianjin University, Tianjin, China., Li W; Pu'er People's Hospital, Yunnan, China., Fang ZH; Pu'er People's Hospital, Yunnan, China., Sun D; Tianjin Chest Hospital, Tianjin, China; Chest Hospital, Tianjin University, Tianjin, China; Tianjin Medical University, Tianjin, China. Electronic address: sdqmd@tju.edu.cn., Guo Z; Tianjin Chest Hospital, Tianjin, China; Chest Hospital, Tianjin University, Tianjin, China; Tianjin Medical University, Tianjin, China. Electronic address: Zhigangguo2022@126.com., Zhang L; School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China. Electronic address: zhanglp@tjcu.edu.cn. |
| المصدر: | Gene [Gene] 2024 Jul 30; Vol. 917, pp. 148467. Date of Electronic Publication: 2024 Apr 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Arabidopsis*/genetics , Gene Expression Regulation, Plant* , Glutathione Peroxidase*/genetics , Glutathione Peroxidase*/metabolism , Plant Roots*/genetics , Plant Roots*/metabolism , Rhodiola*/genetics , Rhodiola*/metabolism, Abscisic Acid/metabolism ; Adaptation, Physiological/genetics ; Antioxidants/metabolism ; Cold Temperature ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Genetically Modified/genetics |
| مستخلص: | Rhodiola crenulata, a plant of great medicinal value found in cold high-altitude regions, has been excessively exploited due to the difficulty in cultivation. Understanding Rhodiola crenulata's adaptation mechanisms to cold environment can provide a theoretical basis for artificial breeding. Glutathione peroxidases (GPXs), critical enzymes found in plants, play essential roles in antioxidant defense through the ascorbate-glutathione cycle. However, it is unknown whether GPX5 contributes to Rhodiola crenulata's cold tolerance. In this study, we investigated the role of GPX5 in Rhodiola crenulata's cold tolerance mechanisms. By overexpressing Rhodiola crenulata GPX5 (RcGPX5) in yeast and Arabidopsis thaliana, we observed down-regulation of Arabidopsis thaliana GPX5 (AtGPX5) and increased cold tolerance in both organisms. Furthermore, the levels of antioxidants and enzyme activities in the ascorbate-glutathione cycle were elevated, and cold-responsive genes such as AtCBFs and AtCORs were induced. Additionally, RcGPX5 overexpressing lines showed insensitivity to exogenous abscisic acid (ABA), suggesting a negative regulation of the ABA pathway by RcGPX5. RcGPX5 also promoted the expression of several thioredoxin genes in Arabidopsis and interacted with two endogenous genes of Rhodiola crenulata, RcTrx2-3 and RcTrxo1, located in mitochondria and chloroplasts. These findings suggest a significantly different model in Rhodiola crenulata compared to Arabidopsis thaliana, highlighting a complex network involving the function of RcGPX5. Moreover, overexpressing RcGPX5 in Rhodiola crenulata hairy roots positively influenced the salidroside synthesis pathway, enhancing its pharmaceutical value for doxorubicin-induced cardiotoxicity. These results suggested that RcGPX5 might be a key component for Rhodiola crenulata to adapt to cold stress and overexpressing RcGPX5 could enhance the pharmaceutical value of the hairy roots. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Abscisic acid; Arabidopsis; Cold tolerance; Doxorubicin-induced cardiotoxicity; RcGPX5; Rhodiola crenulata |
| المشرفين على المادة: | 72S9A8J5GW (Abscisic Acid) 0 (Antioxidants) EC 1.11.1.9 (Glutathione Peroxidase) 0 (Plant Proteins) |
| تواريخ الأحداث: | Date Created: 20240414 Date Completed: 20240515 Latest Revision: 20240807 |
| رمز التحديث: | 20240807 |
| DOI: | 10.1016/j.gene.2024.148467 |
| PMID: | 38615983 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2024.148467 |