Broadly neutralizing antibodies against emerging delta-coronaviruses.
| العنوان: | Broadly neutralizing antibodies against emerging delta-coronaviruses. |
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| المؤلفون: | Rexhepaj M; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Park YJ; Department of Biochemistry, University of Washington, Seattle, Washington, USA.; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Perruzza L; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Asarnow D; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Mccallum M; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Culap K; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Saliba C; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Leoni G; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Balmelli A; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Yoshiyama CN; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Dickinson MS; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Quispe J; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Brown JT; Department of Biochemistry, University of Washington, Seattle, Washington, USA., Tortorici MA; Department of Biochemistry, University of Washington, Seattle, Washington, USA.; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Sprouse KR; Department of Biochemistry, University of Washington, Seattle, Washington, USA.; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Taylor AL; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Starr TN; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Corti D; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Benigni F; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., Veesler D; Department of Biochemistry, University of Washington, Seattle, Washington, USA.; Howard Hughes Medical Institute, Seattle, WA 98195, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Apr 01. Date of Electronic Publication: 2024 Apr 01. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Porcine deltacoronavirus (PDCoV) spillovers were recently detected in children with acute undifferentiated febrile illness, underscoring recurrent zoonoses of divergent coronaviruses. To date, no vaccines or specific therapeutics are approved for use in humans against PDCoV. To prepare for possible future PDCoV epidemics, we isolated human spike (S)-directed monoclonal antibodies from transgenic mice and found that two of them, designated PD33 and PD41, broadly neutralized a panel of PDCoV variants. Cryo-electron microscopy structures of PD33 and PD41 in complex with the PDCoV receptor-binding domain and S ectodomain trimer provide a blueprint of the epitopes recognized by these mAbs, rationalizing their broad inhibitory activity. We show that both mAbs inhibit PDCoV by competitively interfering with host APN binding to the PDCoV receptor-binding loops, explaining the mechanism of viral neutralization. PD33 and PD41 are candidates for clinical advancement, which could be stockpiled to prepare for possible future PDCoV outbreaks. Competing Interests: Declaration of interests D.V. is named as inventors on patents for coronavirus nanoparticle vaccines filed by the University of Washington. D.C., L.P., A.D., K.C., C.S., A.B., and F.B. are employees of Vir Biotechnology and may hold shares in Vir Biotechnology. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
| References: | N Engl J Med. 2020 Feb 20;382(8):727-733. (PMID: 31978945) Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. (PMID: 20057044) Nat Struct Mol Biol. 2019 Jun;26(6):481-489. (PMID: 31160783) Science. 2021 Dec 24;374(6575):1621-1626. (PMID: 34751595) Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5135-E5143. (PMID: 29760102) Elife. 2018 Nov 09;7:. (PMID: 30412051) Nat Med. 2015 Dec;21(12):1508-13. (PMID: 26552008) Cell. 2021 Apr 29;184(9):2332-2347.e16. (PMID: 33761326) J Virol. 2005 Feb;79(3):1595-604. (PMID: 15650185) Nature. 2021 Oct;598(7880):342-347. (PMID: 34464958) Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507) Science. 2020 Mar 13;367(6483):1260-1263. (PMID: 32075877) Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357. (PMID: 28807998) N Engl J Med. 2003 May 15;348(20):1953-66. (PMID: 12690092) J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) Nature. 2023 Dec;624(7990):207-214. (PMID: 37879362) Nat Methods. 2017 Mar;14(3):290-296. (PMID: 28165473) Nature. 2023 Sep;621(7979):592-601. (PMID: 37648855) J Virol. 2018 Jan 30;92(4):. (PMID: 29093093) Nature. 2021 Mar;591(7849):293-299. (PMID: 33494095) Cell. 2020 Apr 16;181(2):281-292.e6. (PMID: 32155444) N Engl J Med. 2012 Nov 8;367(19):1814-20. (PMID: 23075143) Science. 2014 Dec 12;346(6215):1377-80. (PMID: 25504723) Nature. 2021 Jun;594(7862):253-258. (PMID: 33873199) N Engl J Med. 2003 May 15;348(20):1967-76. (PMID: 12690091) Nature. 2021 Sep;597(7874):97-102. (PMID: 34261126) Nature. 2023 Dec;624(7990):201-206. (PMID: 37794193) Nature. 2016 Mar 3;531(7592):118-21. (PMID: 26935699) Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11157-11162. (PMID: 29073020) Nature. 2016 Mar 3;531(7592):114-117. (PMID: 26855426) Nat Struct Mol Biol. 2020 Aug;27(8):763-767. (PMID: 32647346) Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3048-53. (PMID: 26976607) J Mol Biol. 2018 Feb 2;430(3):322-336. (PMID: 29277294) Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002) Adv Virus Res. 2019;105:93-116. (PMID: 31522710) Cell. 2022 Jun 23;185(13):2279-2291.e17. (PMID: 35700730) Cell. 2020 Sep 3;182(5):1295-1310.e20. (PMID: 32841599) J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563) Nat Struct Mol Biol. 2016 Oct;23(10):899-905. (PMID: 27617430) Cell Host Microbe. 2020 Sep 9;28(3):497. (PMID: 32910920) Sci Transl Med. 2021 Jun 30;13(600):. (PMID: 34103407) Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877. (PMID: 31588918) Elife. 2019 Oct 25;8:. (PMID: 31650956) Science. 2021 Sep 17;373(6561):eabj0299. (PMID: 34529476) Nat Commun. 2021 Feb 5;12(1):837. (PMID: 33547281) Sci Immunol. 2022 Dec 23;7(78):eadf1421. (PMID: 36356052) Nat Commun. 2020 Oct 21;11(1):5337. (PMID: 33087721) Nat Med. 2004 Apr;10(4):368-73. (PMID: 15034574) J Virol. 2005 Jan;79(2):884-95. (PMID: 15613317) Nat Commun. 2017 Apr 10;8:15092. (PMID: 28393837) Science. 2003 May 30;300(5624):1394-9. (PMID: 12730500) Proc Soc Exp Biol Med. 1966 Jan;121(1):190-3. (PMID: 4285768) Clin Infect Dis. 2022 Feb 11;74(3):446-454. (PMID: 34013321) Clin Infect Dis. 2022 Aug 24;75(1):e1184-e1187. (PMID: 34718467) Nature. 2021 Dec;600(7887):133-137. (PMID: 34789872) J Struct Biol. 2005 Jul;151(1):41-60. (PMID: 15890530) PLoS Pathog. 2018 Aug 13;14(8):e1007236. (PMID: 30102747) Cell. 2020 Aug 20;182(4):828-842.e16. (PMID: 32645326) Cell. 2020 Nov 12;183(4):1024-1042.e21. (PMID: 32991844) Nat Methods. 2019 Nov;16(11):1146-1152. (PMID: 31591575) Science. 2022 Feb 25;375(6583):864-868. (PMID: 35076256) Science. 2021 Mar 25;:. (PMID: 33766944) Science. 2003 May 30;300(5624):1399-404. (PMID: 12730501) Protein Sci. 2018 Jan;27(1):14-25. (PMID: 28710774) Cell. 2019 Feb 21;176(5):1026-1039.e15. (PMID: 30712865) |
| معلومات مُعتمدة: | 75N93022C00036 United States AI NIAID NIH HHS; DP1 AI158186 United States AI NIAID NIH HHS; P01 AI167966 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20240415 Latest Revision: 20240429 |
| رمز التحديث: | 20240429 |
| مُعرف محوري في PubMed: | PMC11014491 |
| DOI: | 10.1101/2024.03.27.586411 |
| PMID: | 38617231 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2024.03.27.586411 |
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