RNA-sequencing exploration on SIR2 and SOD genes in Polyalthia longifolia leaf methanolic extracts (PLME) mediated anti-aging effects in Saccharomyces cerevisiae BY611 yeast cells.

التفاصيل البيبلوغرافية
العنوان:	RNA-sequencing exploration on SIR2 and SOD genes in Polyalthia longifolia leaf methanolic extracts (PLME) mediated anti-aging effects in Saccharomyces cerevisiae BY611 yeast cells.
المؤلفون:	Hemagirri M; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia USM, 11800, Pulau Pinang, Malaysia., Chen Y; Department of Oral & Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, 50603, Kuala Lumpur, Malaysia., Gopinath SCB; Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia.; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia.; Micro System Technology, Centre of Excellence (CoE), Universiti Malaysia Perlis (UniMAP), Pauh Campus, 02600, Arau, Perlis, Malaysia.; Department of Computer Science and Engineering, Faculty of Science and Information Technology, Daffodil International University, Daffodil Smart City, Birulia, Savar, Dhaka, 1216, Bangladesh., Adnan M; Department of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia., Patel M; Research and Development Cell, Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Vadodara, 391760, India., Sasidharan S; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia USM, 11800, Pulau Pinang, Malaysia. srisasidharan@yahoo.com.
المصدر:	Biogerontology [Biogerontology] 2024 Aug; Vol. 25 (4), pp. 705-737. Date of Electronic Publication: 2024 Apr 15.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 100930043 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6768 (Electronic) Linking ISSN: 13895729 NLM ISO Abbreviation: Biogerontology Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Dordrecht ; Boston : Kluwer Academic, c2000-
مواضيع طبية MeSH:	Plant Extracts/pharmacology , Plant Leaves/chemistry , Polyalthia/chemistry , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/drug effects , Sirtuin 2/genetics , Sirtuin 2*/metabolism, Aging/drug effects ; Aging/genetics ; Gene Expression Regulation, Fungal/drug effects ; Methanol/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Sequence Analysis, RNA/methods ; Silent Information Regulator Proteins, Saccharomyces cerevisiae/genetics ; Silent Information Regulator Proteins, Saccharomyces cerevisiae/metabolism ; Superoxide Dismutase/metabolism ; Superoxide Dismutase/genetics ; Superoxide Dismutase-1/genetics ; Superoxide Dismutase-1/metabolism
مستخلص:	Polyalthia longifolia is well-known for its abundance of polyphenol content and traditional medicinal uses. Previous research has demonstrated that the methanolic extract of P. longifolia leaves (PLME, 1 mg/mL) possesses anti-aging properties in Saccharomyces cerevisiae BY611 yeast cells. Building on these findings, this study delves deeper into the potential antiaging mechanism of PLME, by analyzing the transcriptional responses of BY611 cells treated with PLME using RNA-sequencing (RNA-seq) technology. The RNA-seq analysis results identified 1691 significantly (padj < 0.05) differentially expressed genes, with 947 upregulated and 744 downregulated genes. Notably, the expression of three important aging-related genes, SIR2, SOD1, and SOD2, showed a significant difference following PLME treatment. The subsequent integration of these targeted genes with GO and KEGG pathway analysis revealed the multifaceted nature of PLME's anti-aging effects in BY611 yeast cells. Enriched GO and KEGG analysis showed that PLME treatment promotes the upregulation of SIR2, SOD1, and SOD2 genes, leading to a boosted cellular antioxidant defense system, reduced oxidative stress, regulated cell metabolism, and maintain genome stability. These collectively increased longevities in PLME-treated BY611 yeast cells and indicate the potential anti-aging action of PLME through the modulation of SIR2 and SOD genes. The present study provided novel insights into the roles of SIR2, SOD1, and SOD2 genes in the anti-aging effects of PLME treatment, offering promising interventions for promoting healthy aging. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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معلومات مُعتمدة:	1001/CIPPM /8012229 Universiti Sains Malaysia
فهرسة مساهمة:	Keywords: Polyalthia longifolia; SIR2; SOD genes; Saccharomyces cerevisiae; Anti-aging; RNA sequencing; Transcriptomic analysis
المشرفين على المادة:	Y4S76JWI15 (Methanol) 0 (Plant Extracts) 0 (Saccharomyces cerevisiae Proteins) 0 (Silent Information Regulator Proteins, Saccharomyces cerevisiae) EC 3.5.1.- (SIR2 protein, S cerevisiae) EC 3.5.1.- (Sirtuin 2) EC 1.15.1.1 (Superoxide Dismutase) EC 1.15.1.1 (superoxide dismutase 2) EC 1.15.1.1 (Superoxide Dismutase-1)
تواريخ الأحداث:	Date Created: 20240415 Date Completed: 20240701 Latest Revision: 20240725
رمز التحديث:	20240726
DOI:	10.1007/s10522-024-10104-y
PMID:	38619670
قاعدة البيانات:	MEDLINE

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تدمد:	1573-6768
DOI:	10.1007/s10522-024-10104-y