دورية أكاديمية
Molecular-Level Dysregulation of Insulin Pathways and Inflammatory Processes in Peripheral Blood Mononuclear Cells by Circadian Misalignment.
| العنوان: | Molecular-Level Dysregulation of Insulin Pathways and Inflammatory Processes in Peripheral Blood Mononuclear Cells by Circadian Misalignment. |
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| المؤلفون: | McDermott JE; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.; Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, United States., Jacobs JM; Environmental and Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States., Merrill NJ; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States., Mitchell HD; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States., Arshad OA; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States., McClure R; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States., Teeguarden J; Environmental and Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States., Gajula RP; Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, Washington 99202, United States., Porter KI; Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, Washington 99202, United States., Satterfield BC; Sleep and Performance Research Center, Washington State University, Spokane, Washington 99202, United States.; Department of Translational Medicine and Physiology, Washington State University, Spokane, Washington 99202, United States., Lundholm KR; Sleep and Performance Research Center, Washington State University, Spokane, Washington 99202, United States.; Department of Translational Medicine and Physiology, Washington State University, Spokane, Washington 99202, United States., Skene DJ; Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH, United Kingdom., Gaddameedhi S; Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695, United States.; Center for Human Health and the Environment, North Carolina State University, Raleigh, North Carolina 27695, United States., Dongen HPAV; Sleep and Performance Research Center, Washington State University, Spokane, Washington 99202, United States.; Department of Translational Medicine and Physiology, Washington State University, Spokane, Washington 99202, United States. |
| المصدر: | Journal of proteome research [J Proteome Res] 2024 May 03; Vol. 23 (5), pp. 1547-1558. Date of Electronic Publication: 2024 Apr 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: | Leukocytes, Mononuclear*/metabolism , Insulin*/metabolism , Insulin*/blood , Circadian Rhythm* , Inflammation*/metabolism , Inflammation*/blood, Humans ; Male ; Adult ; Shift Work Schedule ; Female ; Proteomics/methods ; Blood Glucose/metabolism ; Signal Transduction ; Insulin Resistance ; Young Adult |
| مستخلص: | Circadian misalignment due to night work has been associated with an elevated risk for chronic diseases. We investigated the effects of circadian misalignment using shotgun protein profiling of peripheral blood mononuclear cells taken from healthy humans during a constant routine protocol, which was conducted immediately after participants had been subjected to a 3-day simulated night shift schedule or a 3-day simulated day shift schedule. By comparing proteomic profiles between the simulated shift conditions, we identified proteins and pathways that are associated with the effects of circadian misalignment and observed that insulin regulation pathways and inflammation-related proteins displayed markedly different temporal patterns after simulated night shift. Further, by integrating the proteomic profiles with previously assessed metabolomic profiles in a network-based approach, we found key associations between circadian dysregulation of protein-level pathways and metabolites of interest in the context of chronic metabolic diseases. Endogenous circadian rhythms in circulating glucose and insulin differed between the simulated shift conditions. Overall, our results suggest that circadian misalignment is associated with a tug of war between central clock mechanisms controlling insulin secretion and peripheral clock mechanisms regulating insulin sensitivity, which may lead to adverse long-term outcomes such as diabetes and obesity. Our study provides a molecular-level mechanism linking circadian misalignment and adverse long-term health consequences of night work. |
| معلومات مُعتمدة: | R01 ES030113 United States ES NIEHS NIH HHS |
| فهرسة مساهمة: | Keywords: circadian misalignment; glucose regulation; human; metabolomics; proteomics; sleep |
| المشرفين على المادة: | 0 (Insulin) 0 (Blood Glucose) |
| تواريخ الأحداث: | Date Created: 20240415 Date Completed: 20240503 Latest Revision: 20240523 |
| رمز التحديث: | 20240523 |
| DOI: | 10.1021/acs.jproteome.3c00418 |
| PMID: | 38619923 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1535-3907 |
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| DOI: | 10.1021/acs.jproteome.3c00418 |