دورية أكاديمية
أعرض في EDS

Lab on a bead with oscillatory centrifugal microfluidics for fast and complete mixing enables fast and accurate biomedical assays.

التفاصيل البيبلوغرافية
العنوان: Lab on a bead with oscillatory centrifugal microfluidics for fast and complete mixing enables fast and accurate biomedical assays.
المؤلفون: Williams DE; School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand. david.williams@auckland.ac.nz., Li W; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Chandrasekhar M; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Corazza CMOW; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Deijs GS; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Djoko L; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Govind B; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Jose E; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Kwon YJ; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Lowe T; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Panchal A; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Reshef G; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Vargas MJT; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Simpson MC; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand. c.simpson@auckland.ac.nz.; School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand. c.simpson@auckland.ac.nz.
المصدر: Scientific reports [Sci Rep] 2024 Apr 15; Vol. 14 (1), pp. 8637. Date of Electronic Publication: 2024 Apr 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Microfluidics*/methods , Antibodies*, Antigens ; Point-of-Care Systems ; Hydrophobic and Hydrophilic Interactions
مستخلص: Rapid mixing and precise timing are key for accurate biomedical assay measurement, particularly when the result is determined as the rate of a reaction: for example rapid immunoassay in which the amount of captured target is kinetically determined; determination of the concentration of an enzyme or enzyme substrate; or as the final stage in any procedure that involves a capture reagent when an enzyme reaction is used as the indicator. Rapid mixing and precise timing are however difficult to achieve in point-of-care devices designed for small sample volumes and fast time to result. By using centrifugal microfluidics and transposing the reaction surface from a chamber to a single mm-scale bead we demonstrate an elegant and easily manufacturable solution. Reagents (which may be, for example, an enzyme, enzyme substrate, antibody or antigen) are immobilised on the surface of a single small bead (typically 1-2 mm in diameter) contained in a cylindrical reaction chamber subjected to periodically changing rotational accelerations which promote both mixing and uniform mass-transfer to the bead surface. The gradient of Euler force across the chamber resulting from rotational acceleration of the disc, dΩ disc /dt, drives circulation of fluid in the chamber. Oscillation of Euler force by oscillation of rotational acceleration with period, T, less than that of the hydrodynamic relaxation time of the fluid, folds the fluid streamlines. Movement of the bead in response to the fluid and the changing rotational acceleration provides a dynamically changing chamber shape, further folding and expanding the fluid. Bead rotation and translation driven by fluid flow and disc motion give uniformity of reaction over the surface. Critical parameters for mixing and reaction uniformity are the ratio of chamber radius to bead radius, r chamber /r bead , and the product Tr chamber (dΩ disc /dt), of oscillation period and Euler force gradient across the fluid. We illustrate application of the concept using the reaction of horse radish peroxidase (HRP) immobilised on the bead surface with its substrate tetramethylbenzidine (TMB) in solution. Acceleration from rest to break a hydrophobic valve provided precise timing for TMB contact with the bead. Solution uniformity from reaction on the surface of the bead in volumes 20-50 uL was obtained in times of 2.5 s or less. Accurate measurement of the amount of surface-bound HRP by model fitting to the measured kinetics of colour development at 10 s intervals is demonstrated.
(© 2024. The Author(s).)
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المشرفين على المادة: 0 (Antibodies)
0 (Antigens)
تواريخ الأحداث: Date Created: 20240415 Date Completed: 20240417 Latest Revision: 20240425
رمز التحديث: 20240425
مُعرف محوري في PubMed: PMC11018808
DOI: 10.1038/s41598-024-58720-5
PMID: 38622241
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-58720-5