دورية أكاديمية
Lab on a bead with oscillatory centrifugal microfluidics for fast and complete mixing enables fast and accurate biomedical assays.
العنوان: | Lab on a bead with oscillatory centrifugal microfluidics for fast and complete mixing enables fast and accurate biomedical assays. |
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المؤلفون: | Williams DE; School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand. david.williams@auckland.ac.nz., Li W; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Chandrasekhar M; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Corazza CMOW; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Deijs GS; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Djoko L; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Govind B; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Jose E; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Kwon YJ; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Lowe T; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Panchal A; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Reshef G; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Vargas MJT; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand., Simpson MC; Orbis Diagnostics Ltd, 14 West St, Eden Terrace, Auckland, 1010, New Zealand. c.simpson@auckland.ac.nz.; School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand. c.simpson@auckland.ac.nz. |
المصدر: | Scientific reports [Sci Rep] 2024 Apr 15; Vol. 14 (1), pp. 8637. Date of Electronic Publication: 2024 Apr 15. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Microfluidics*/methods , Antibodies*, Antigens ; Point-of-Care Systems ; Hydrophobic and Hydrophilic Interactions |
مستخلص: | Rapid mixing and precise timing are key for accurate biomedical assay measurement, particularly when the result is determined as the rate of a reaction: for example rapid immunoassay in which the amount of captured target is kinetically determined; determination of the concentration of an enzyme or enzyme substrate; or as the final stage in any procedure that involves a capture reagent when an enzyme reaction is used as the indicator. Rapid mixing and precise timing are however difficult to achieve in point-of-care devices designed for small sample volumes and fast time to result. By using centrifugal microfluidics and transposing the reaction surface from a chamber to a single mm-scale bead we demonstrate an elegant and easily manufacturable solution. Reagents (which may be, for example, an enzyme, enzyme substrate, antibody or antigen) are immobilised on the surface of a single small bead (typically 1-2 mm in diameter) contained in a cylindrical reaction chamber subjected to periodically changing rotational accelerations which promote both mixing and uniform mass-transfer to the bead surface. The gradient of Euler force across the chamber resulting from rotational acceleration of the disc, dΩ (© 2024. The Author(s).) |
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المشرفين على المادة: | 0 (Antibodies) 0 (Antigens) |
تواريخ الأحداث: | Date Created: 20240415 Date Completed: 20240417 Latest Revision: 20240425 |
رمز التحديث: | 20240425 |
مُعرف محوري في PubMed: | PMC11018808 |
DOI: | 10.1038/s41598-024-58720-5 |
PMID: | 38622241 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-024-58720-5 |