دورية أكاديمية
أعرض في EDS

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh.

التفاصيل البيبلوغرافية
العنوان: Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh.
المؤلفون: Islam R; Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh, Bangladesh., Ferdous FB; Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh, Bangladesh., Hoque MN; Department of Gynecology, Obstetrics and Reproductive Health, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh., Asif NA; Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rana ML; Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh, Bangladesh., Siddique MP; Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rahman MT; Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh, Bangladesh.
المصدر: PloS one [PLoS One] 2024 Apr 16; Vol. 19 (4), pp. e0296542. Date of Electronic Publication: 2024 Apr 16 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/therapeutic use , Pseudomonas Infections*/epidemiology , Pseudomonas Infections*/veterinary , Pseudomonas Infections*/drug therapy, Humans ; Animals ; Pseudomonas aeruginosa ; beta-Lactamases/genetics ; beta-Lactamases/therapeutic use ; Virulence/genetics ; Hospitals, Animal ; Bangladesh ; Poultry ; Hospitals, Teaching ; Microbial Sensitivity Tests
مستخلص: The emergence and spread of multidrug-resistant pathogens like Pseudomonas aeruginosa are major concerns for public health worldwide. This study aimed to assess the prevalence of P. aeruginosa in clinical, environmental, and poultry sources in Bangladesh, along with their antibiotic susceptibility and the profiling of β-lactamase and virulence genes using standard molecular and microbiology techniques. We collected 110 samples from five different locations, viz., BAU residential area (BAURA; n = 15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n = 22), Poultry Market (PM; n = 30) and Mymensingh Medical College Hospital (MCCH; n = 23). After overnight enrichment in nutrient broth, 89 probable Pseudomonas isolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) as P. aeruginosa from these samples. Antibiogram profiling revealed that 100.0% P. aeruginosa isolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However, P. aeruginosa isolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. The blaTEM gene was detected in 86.0% isolates, while blaCMY, blaSHV and blaOXA, were detected in 27.0%, 18.0% and 5.0% of the P. aeruginosa isolates, respectively. The algD gene was detected in 32.0% isolates, whereas lasB and exoA genes were identified in 9.0% and 5.0% P. aeruginosa isolates. However, none of the P. aeruginosa isolates harbored exoS gene. Hence, this study provides valuable and novel insights on the resistance and virulence of circulating P. aeruginosa within the clinical, environmental, and poultry environments of Bangladesh. These findings are crucial for understanding the emergence of β-lactamase resistance in P. aeruginosa, highlighting its usefulness in the treatment and control of P. aeruginosa infections in both human and animal populations.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Islam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Anti-Bacterial Agents)
EC 3.5.2.6 (beta-Lactamases)
تواريخ الأحداث: Date Created: 20240416 Date Completed: 20240418 Latest Revision: 20240425
رمز التحديث: 20240425
مُعرف محوري في PubMed: PMC11020970
DOI: 10.1371/journal.pone.0296542
PMID: 38626002
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0296542