دورية أكاديمية
Adipose tissue IL-18 production is independent of caspase-1 and caspase-11.
| العنوان: | Adipose tissue IL-18 production is independent of caspase-1 and caspase-11. |
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| المؤلفون: | Román-Domínguez L; Laboratorio de Neuroinmunobiología, Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico., Salazar-León J; Laboratorio de Neuroinmunobiología, Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico., Meza-Sosa KF; Laboratorio de Neuroinmunobiología, Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico., Pérez-Martínez L; Laboratorio de Neuroinmunobiología, Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico., Pedraza-Alva G; Laboratorio de Neuroinmunobiología, Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico. |
| المصدر: | Immunity, inflammation and disease [Immun Inflamm Dis] 2024 Apr; Vol. 12 (4), pp. e1241. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley and Sons Ltd Country of Publication: England NLM ID: 101635460 Publication Model: Print Cited Medium: Internet ISSN: 2050-4527 (Electronic) Linking ISSN: 20504527 NLM ISO Abbreviation: Immun Inflamm Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Oxford] : John Wiley and Sons Ltd, [2013]- |
| مواضيع طبية MeSH: | Adipose Tissue*/metabolism , Caspase 1*/metabolism , Interleukin-18*/metabolism , NLR Family, Pyrin Domain-Containing 3 Protein*/genetics , NLR Family, Pyrin Domain-Containing 3 Protein*/metabolism , Caspases, Initiator*/metabolism, Animals ; Mice ; Caspases/metabolism ; Cytokines/metabolism ; Inflammasomes/metabolism ; Insulin Resistance ; Lipids |
| مستخلص: | Background: Inflammation in adipose tissue, resulting from imbalanced caloric intake and energy expenditure, contributes to the metabolic dysregulation observed in obesity. The production of inflammatory cytokines, such as IL-1β and IL-18, plays a key role in this process. While IL-1β promotes insulin resistance and diabetes, IL-18 regulates energy expenditure and food intake. Previous studies have suggested that caspase-1, activated by the Nlrp3 inflammasome in response to lipid excess, mediates IL-1β production, whereas activated by the Nlrp1b inflammasome in response to energy excess, mediates IL-18 production. However, this has not been formally tested. Methods: Wild-type and caspase-1-deficient Balb/c mice, carrying the Nlrp1b1 allele, were fed with regular chow or a high-fat diet for twelve weeks. Food intake and mass gain were recorded weekly. At the end of the twelve weeks, glucose tolerance and insulin resistance were evaluated. Mature IL-18 protein levels and the inflammatory process in the adipose tissue were determined. Fasting lipid and cytokine levels were quantified in the sera of the different experimental groups. Results: We found that IL-18 production in adipose tissue is independent of caspase-1 activity, regardless of the metabolic state, while Nlrp3-mediated IL-1β production remains caspase-1 dependent. Additionally, caspase-1 null Balb/c mice did not develop metabolic abnormalities in response to energy excess from the high-fat diet. Conclusion: Our findings suggest that IL-18 production in the adipose tissue is independent of Nlrp3 inflammasome and caspase-1 activation, regardless of caloric food intake. In contrast, Nlrp3-mediated IL-1β production is caspase-1 dependent. These results provide new insights into the mechanisms underlying cytokine production in the adipose tissue during both homeostatic conditions and metabolic stress, highlighting the distinct roles of caspase-1 and the Nlrp inflammasomes in regulating inflammatory responses. (© 2024 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | IN211719 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México; IN213119 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México; IN216922 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México; IN217822 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México; CF-2019/40792 Consejo Nacional de Ciencia y Tecnología; IFC-2016/2282 Consejo Nacional de Ciencia y Tecnología |
| فهرسة مساهمة: | Keywords: adipose tissue; animals; cytokines; diabetes; inflammation; macrophage; molecules |
| المشرفين على المادة: | EC 3.4.22.36 (Caspase 1) EC 3.4.22.- (Caspases) 0 (Cytokines) 0 (Inflammasomes) 0 (Interleukin-18) 0 (Lipids) 0 (NLR Family, Pyrin Domain-Containing 3 Protein) EC 3.4.22.- (Casp4 protein, mouse) EC 3.4.22.- (Caspases, Initiator) |
| تواريخ الأحداث: | Date Created: 20240417 Date Completed: 20240418 Latest Revision: 20240501 |
| رمز التحديث: | 20240501 |
| مُعرف محوري في PubMed: | PMC11022623 |
| DOI: | 10.1002/iid3.1241 |
| PMID: | 38629728 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2050-4527 |
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| DOI: | 10.1002/iid3.1241 |