دورية أكاديمية

Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts.

التفاصيل البيبلوغرافية
العنوان: Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts.
المؤلفون: Nithyapriya S; PG and Research Department of Botany, Padmavani Arts and Science College for Women, Salem, 636011, India., Sundaram L; Department of Botany, Periyar University, Salem, 636011, India. lara9k@gmail.com., Eswaran SUD; Department of Botany, Periyar University, Salem, 636011, India., Perveen K; Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, 11451, Riyadh, Saudi Arabia., Alshaikh NA; Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, 11451, Riyadh, Saudi Arabia., Sayyed RZ; Department of Microbiology, PSGVP Mandal's S I Patil Arts, G B Patel Science and STKV Sangh Commerce College, Shahada, 425409, India. sayyedrz@gmail.com.; Faculty of Health and Life Sciences, INTI International University, Negeri Sembilan, Persiaran Perdana BBN, Putra Nilai, 71800, Nilai, Malaysia. sayyedrz@gmail.com., Mastinu A; Department of Molecular and Translational Medicine, Division of Pharmacology, University of Brescia, 25123, Brescia, Italy. andrea.mastinu@unibs.it.
المصدر: Microbial ecology [Microb Ecol] 2024 Apr 17; Vol. 87 (1), pp. 60. Date of Electronic Publication: 2024 Apr 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: United States NLM ID: 7500663 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-184X (Electronic) Linking ISSN: 00953628 NLM ISO Abbreviation: Microb Ecol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Springer-Verlag.
مواضيع طبية MeSH: Arachis* , Pseudomonas fluorescens*, Deferoxamine ; India ; RNA, Ribosomal, 16S/genetics ; Nutrients ; Siderophores ; Iron ; Soil
مستخلص: Microorganisms produce siderophores, which are low-molecular-weight iron chelators when iron availability is limited. The present analyzed the role of LNPF1 as multifarious PGPR for improving growth parameters and nutrient content in peanut and soil nutrients. Such multifarious PGPR strains can be used as effective bioinoculants for peanut farming. In this work, rhizosphere bacteria from Zea mays and Arachis hypogaea plants in the Salem area of Tamil Nadu, India, were isolated and tested for biochemical attributes and characteristics that stimulate plant growth, such as the production of hydrogen cyanide, ammonia (6 µg/mL), indole acetic acid (76.35 µg/mL), and solubilizing phosphate (520 µg/mL). The 16S rRNA gene sequences identified the isolate LNPF1 as Pseudomonas fluorescens with a similarity percentage of 99% with Pseudomonas sp. Isolate LNPF1 was evaluated for the production of siderophore. Siderophore-rich supernatant using a Sep Pack C18 column and Amberlite-400 Resin Column (λmax 264) produced 298 mg/L and 50 mg/L of siderophore, respectively. The characterization of purified siderophore by TLC, HPLC, FTIR, and 2D-NMR analysis identified the compound as desferrioxamine, a hydroxamate siderophore. A pot culture experiment determined the potential of LNPF1 to improve iron and oil content and photosynthetic pigments in Arachis hypogaea L. and improve soil nutrient content. Inoculation of A. hypogea seeds with LNPF1 improved plant growth parameters such as leaf length (60%), shoot length (22%), root length (54.68%), fresh weight (47.28%), dry weight (37%), and number of nuts (66.66) compared to the control (untreated seeds). This inoculation also improved leaf iron content (43.42), short iron content (38.38%), seed iron (46.72%), seed oil (31.68%), carotenoid (64.40%), and total chlorophyll content (98.%) compared to control (untreated seeds). Bacterized seeds showed a substantial increase in nodulation (61.65%) and weight of individual nodules (95.97) vis-à-vis control. The results of the present study indicated that P. fluorescens might be utilized as a potential bioinoculant to improve growth, iron content, oil content, number of nuts and nodules of Arachishypogaea L., and enrich soil nutrients.
(© 2024. The Author(s).)
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معلومات مُعتمدة: RSP2024R358 King Saud University
فهرسة مساهمة: Keywords: Characterization; Hydroxamate; Iron nutrition; Plant growth promotion; Purification; Siderophore
المشرفين على المادة: J06Y7MXW4D (Deferoxamine)
0 (RNA, Ribosomal, 16S)
0 (Siderophores)
E1UOL152H7 (Iron)
0 (Soil)
تواريخ الأحداث: Date Created: 20240417 Date Completed: 20240418 Latest Revision: 20240425
رمز التحديث: 20240425
مُعرف محوري في PubMed: PMC11024037
DOI: 10.1007/s00248-024-02377-0
PMID: 38630182
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-184X
DOI:10.1007/s00248-024-02377-0