دورية أكاديمية
Bu-Shen-Ning-Xin decoction inhibits macrophage activation to ameliorate premature ovarian insufficiency-related osteoimmune disorder via FSH/FSHR pathway.
| العنوان: | Bu-Shen-Ning-Xin decoction inhibits macrophage activation to ameliorate premature ovarian insufficiency-related osteoimmune disorder via FSH/FSHR pathway. |
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| المؤلفون: | Sun H; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China.; Hexi University, Zhangye, Gansu, China., Qi Q; Wuhan Business University, Wuhan, Hubei, China., Pan X; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China., Zhou J; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China., Wang J; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China., Li L; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China., Li D; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China., Wang L; Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.; The Academy of Integrative Medicine of Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, Shanghai, China. |
| المصدر: | Drug discoveries & therapeutics [Drug Discov Ther] 2024 Jun 06; Vol. 18 (2), pp. 106-116. Date of Electronic Publication: 2024 Apr 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Shandong University China-Japan Cooperation Center for Drug Discovery & Screen Country of Publication: Japan NLM ID: 101493809 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1881-784X (Electronic) Linking ISSN: 18817831 NLM ISO Abbreviation: Drug Discov Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Tokyo] : Shandong University China-Japan Cooperation Center for Drug Discovery & Screen : International Advancement Center for Medicine & Health Research |
| مواضيع طبية MeSH: | Primary Ovarian Insufficiency*/drug therapy , Primary Ovarian Insufficiency*/chemically induced , Drugs, Chinese Herbal*/pharmacology , Drugs, Chinese Herbal*/therapeutic use , Macrophage Activation*/drug effects , Follicle Stimulating Hormone*/blood , Disease Models, Animal* , Receptors, FSH*/metabolism, Animals ; Female ; Mice ; Vinyl Compounds/pharmacology ; Vinyl Compounds/therapeutic use ; Network Pharmacology ; Cyclohexenes/pharmacology ; Cyclohexenes/therapeutic use ; Signal Transduction/drug effects ; Humans ; Osteoclasts/drug effects ; Osteoclasts/metabolism ; Cell Differentiation/drug effects |
| مستخلص: | Limited studies are associated with premature ovarian insufficiency (POI)-related osteoimmune disorder currently. Bu-Shen-Ning-Xin decoction (BSNXD) displayed a favorable role in treating postmenopausal osteoporosis. However, its impact on the POI-related osteoimmune disorder remains unclear. The study primarily utilized animal experiments and network pharmacology to investigate the effects and underlying mechanisms of BSNXD on the POI-related osteoimmune disorder. First, a 4-vinylcyclohexene dioxide (VCD)-induced POI murine model was conducted to explore the therapeutical action of BSNXD. Second, we analyzed the active compounds of BSNXD and predicted their potential mechanisms for POI-related osteoimmune disorder via network pharmacology, further confirmed by molecular biology experiments. The results demonstrated that VCD exposure led to elevated follicle-stimulating hormone (FSH) levels, a 50% reduction in the primordial follicles, bone microstructure changes, and macrophage activation, indicating an osteoimmune disorder. BSNXD inhibited macrophage activation and osteoclast differentiation but did not affect serum FSH and estradiol levels in the VCD-induced POI model. Network pharmacology predicted the potential mechanisms of BSNXD against the POI-related osteoimmune disorder involving tumor necrosis factor α and MAPK signaling pathways, highlighting BSNXD regulated inflammation, hormone, and osteoclast differentiation. Further experiments identified BSNXD treatment suppressed macrophage activation via downregulating FSH receptor (FSHR) expression and inhibiting the phosphorylation of ERK and CCAAT enhancer binding proteins β. In conclusion, BSNXD regulated POI-related osteoimmune disorder by suppressing the FSH/FSHR pathway to reduce macrophage activation and further inhibiting osteoclastogenesis. |
| فهرسة مساهمة: | Keywords: Bu-Shen-Ning-Xin decoction; macrophages; network pharmacology; osteoclastogenesis; osteoimmune disorder; premature ovarian insufficiency |
| المشرفين على المادة: | 0 (Drugs, Chinese Herbal) 9002-68-0 (Follicle Stimulating Hormone) 0 (Receptors, FSH) 596C064IG4 (4-vinyl-1-cyclohexene dioxide) 0 (Vinyl Compounds) 0 (Cyclohexenes) |
| تواريخ الأحداث: | Date Created: 20240417 Date Completed: 20240605 Latest Revision: 20240613 |
| رمز التحديث: | 20240613 |
| DOI: | 10.5582/ddt.2024.01006 |
| PMID: | 38631868 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1881-784X |
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| DOI: | 10.5582/ddt.2024.01006 |