دورية أكاديمية
Treponema pallidum PCR with blood and cerebrospinal fluid of newborns exposed and not exposed to syphilis during pregnancy.
| العنوان: | Treponema pallidum PCR with blood and cerebrospinal fluid of newborns exposed and not exposed to syphilis during pregnancy. |
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| المؤلفون: | Melo RCC; Sanitary Dermatology Outpatient Clinic, State Health Department of Rio Grande do Sul, Brazil., Ramos MC; Sanitary Dermatology Outpatient Clinic, State Health Department of Rio Grande do Sul, Brazil., Rossetti MLR; Molecular Biology Laboratory of Universidade Luterana do Brasil, Canoas, Brazil., Grassi VMT; Molecular Biology Laboratory of Universidade Luterana do Brasil, Canoas, Brazil., Colvero MO; Hospital Santa Casa de Misericórdia de Porto Alegre, Brazil., Marzarotto TAD; Hospital Santa Casa de Misericórdia de Porto Alegre, Brazil., Bonamigo RR; Pathology Postgraduate of Universidade Federal de Ciências da Saúde de Porto Alegre, Brazil. |
| المصدر: | Journal of infection in developing countries [J Infect Dev Ctries] 2024 Mar 31; Vol. 18 (3), pp. 420-426. Date of Electronic Publication: 2024 Mar 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Open Learning on Enteric Pathogens Country of Publication: Italy NLM ID: 101305410 Publication Model: Electronic Cited Medium: Internet ISSN: 1972-2680 (Electronic) Linking ISSN: 19722680 NLM ISO Abbreviation: J Infect Dev Ctries Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Italy?] : Open Learning on Enteric Pathogens |
| مواضيع طبية MeSH: | Syphilis*/diagnosis , Pregnancy Complications, Infectious*/diagnosis , Syphilis, Congenital*/diagnosis, Pregnancy ; Female ; Infant, Newborn ; Humans ; Treponema pallidum/genetics ; Cross-Sectional Studies ; Polymerase Chain Reaction |
| مستخلص: | Introduction: Congenital syphilis (CS) has severe adverse outcomes, including abortion and death. Diagnosis of CS in asymptomatic newborns remains difficult. This study aims to evaluate an in-house polymerase chain reaction (PCR) on cerebrospinal fluid (CSF) and blood samples (BS) to identify T. pallidum DNA in newborns. Methodology: We performed an exploratory cross-sectional study that included newborns exposed to syphilis during pregnancy (SEG) and non-exposed (SNEG) newborns, between 2019 and 2020. In-house conventional PCR for T. pallidum targeting the tpp47 gene was used to analyze CSFS and dried blood spots. Results: BS was obtained from 54 newborns (33 SEG/21 SNEG) and CSF from 55 newborns (33 SEG/22 SNEG). Twenty-five (71.4%) SEG newborns had reactive BS rapid plasmatic reagins (RPR), and all of them had RPR titers less than or equal to the corresponding maternal titers. All RPR CSF tests were negative. PCR for T. pallidum DNA was positive in 19/33 (57.6%) BS, and in 22/33 CSF. The only SEG newborn with clinical signs of early CS had a positive CSF PCR and a negative BS PCR. Conversely, among SNEG newborns, PCR was positive in 2/21 BS and 5/22 (22.7%) CSF. Conclusions: T. pallidum DNA was identified using our PCR tests. The exposed group did not present abnormalities that would indicate CS. This prevented conclusions regarding sensitivity and specificity. Dried spot permitted bedside collection, easy transportation, and storage. Further research is needed to evaluate and improve the accuracy of CS low-cost PCR tests, especially for limited resource settings. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2024 Rafaela Caroline C Melo, Mauro C Ramos, Maria Lúcia R Rossetti, Vera Mileide T Grassi, Maurício O Colvero, Thales Augusto DT Marzarotto, Renan R Bonamigo.) |
| فهرسة مساهمة: | Keywords: PCR; congenital; diagnosis; molecular; syphilis |
| تواريخ الأحداث: | Date Created: 20240418 Date Completed: 20240422 Latest Revision: 20240422 |
| رمز التحديث: | 20240422 |
| DOI: | 10.3855/jidc.17520 |
| PMID: | 38635616 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1972-2680 |
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| DOI: | 10.3855/jidc.17520 |