دورية أكاديمية
أعرض في EDS

GNAI3 mediated by Lin28A regulates lipopolysaccharide-induced inflammation and osteogenic differentiation in periodontal stem cells by mediating the NF-κB/NLRP3 inflammasome pathway.

التفاصيل البيبلوغرافية
العنوان: GNAI3 mediated by Lin28A regulates lipopolysaccharide-induced inflammation and osteogenic differentiation in periodontal stem cells by mediating the NF-κB/NLRP3 inflammasome pathway.
المؤلفون: Guo L; Stomatology Clinic, MeiZhou People's Hospital, Meizhou Academy of Medical Sciences, Meizhou, Guangdong 514000, China. Electronic address: Guoling_2021@163.com., Sun H; Stomatology Clinic, MeiZhou People's Hospital, Meizhou Academy of Medical Sciences, Meizhou, Guangdong 514000, China., Pu J; Stomatology Clinic, MeiZhou People's Hospital, Meizhou Academy of Medical Sciences, Meizhou, Guangdong 514000, China.
المصدر: Archives of oral biology [Arch Oral Biol] 2024 Jul; Vol. 163, pp. 105974. Date of Electronic Publication: 2024 Apr 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Pergamon Press
Original Publication: Oxford, New York [etc.] Pergamon Press.
مواضيع طبية MeSH: Cell Differentiation*/drug effects , Inflammasomes*/metabolism , NF-kappa B*/metabolism , NLR Family, Pyrin Domain-Containing 3 Protein*/metabolism , Osteogenesis*/drug effects , Periodontal Ligament*/cytology , Periodontal Ligament*/metabolism , RNA-Binding Proteins*/metabolism , Stem Cells*/metabolism , Stem Cells*/drug effects , GTP-Binding Protein alpha Subunits, Gi-Go*/metabolism, Humans ; Apoptosis/drug effects ; Blotting, Western ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Inflammation/metabolism ; Lipopolysaccharides/pharmacology ; Periodontitis/metabolism ; Real-Time Polymerase Chain Reaction ; Signal Transduction
مستخلص: Objectives: The aim of this study was to investigate the regulatory role of G protein subunit alpha i3 (GNAI3) in periodontitis.
Design: Following the induction of human periodontal ligament stem cells (hPDLSCs) with lipopolysaccharide (LPS), the mRNA and protein expressions of GNAI3 and Lin28A were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. The transfection efficiency of Oe-GNAI3 and sh-Lin28A was examined by virtue of RT-qPCR and western blot. With the application of ELISA and flow cytometry, the releases of inflammatory cytokines and cell apoptosis were appraised. Alkaline phosphatase (ALP) staining and alizarin red S (ARS) staining were conducted to evaluate osteogenic differentiation. Next, the binding ability of Lin28A with GNAI3 mRNA was estimated by radioimmunoprecipitation (RIP) assay while the stability of GNAI3 mRNA was assessed utilizing RT-qPCR. Western blot was employed for the measurement of inflammation-, apoptosis- and nuclear factor-kappaB (NF-κB)/NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome pathway-related proteins and osteogenic markers.
Results: The expression of GNAI3 was down-regulated in LPS-induced hPDLSCs. After the transfection with Oe-GNAI3, the inflammation and apoptosis in LPS-induced hPDLSCs were inhibited while osteogenic differentiation was promoted. Moreover, Lin28A could stabilize GNAI3 mRNA and Lin28A knockdown significantly reduced GNAI3 expression. Further experiments verified that the inhibitory effects of GNAI3 overexpression on LPS-induced cellular inflammation and cell apoptosis as well as the promotive effects on osteogenic differentiation in hPDLSCs were all partially counteracted by Lin28A depletion, which may possibly be mediated via the regulation of the NF-κB/NLRP3 inflammasome pathway.
Conclusion: GNAI3 that mediated by Lin28A regulates the inflammation and osteogenic differentiation in LPS-induced hPDLSCs by mediating the NF-κB/NLRP3 inflammasome pathway.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: GNAI3; Inflammation; Lin28A; NF-κB/NLRP3 inflammasome pathway; Osteogenic differentiation; Periodontitis
المشرفين على المادة: 0 (Inflammasomes)
0 (Lin28A protein, human)
0 (Lipopolysaccharides)
0 (NF-kappa B)
0 (NLR Family, Pyrin Domain-Containing 3 Protein)
0 (NLRP3 protein, human)
0 (RNA-Binding Proteins)
EC 3.6.5.1 (GNAI1 protein, human)
EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
تواريخ الأحداث: Date Created: 20240418 Date Completed: 20240510 Latest Revision: 20240531
رمز التحديث: 20240531
DOI: 10.1016/j.archoralbio.2024.105974
PMID: 38636252
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1506
DOI:10.1016/j.archoralbio.2024.105974