دورية أكاديمية
Assessing the impact of COVID-19 era drug combinations on hepatic functionality: A thorough investigation in adult Danio rerio.
| العنوان: | Assessing the impact of COVID-19 era drug combinations on hepatic functionality: A thorough investigation in adult Danio rerio. |
|---|---|
| المؤلفون: | Onofre-Camarena DB; Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón Intersección Paseo Tollocan, Colonia Residencial Colón, CP, 50120, Toluca, Estado de México, Mexico., Elizalde-Velázquez GA; Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón Intersección Paseo Tollocan, Colonia Residencial Colón, CP, 50120, Toluca, Estado de México, Mexico., Gómez-Oliván LM; Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón Intersección Paseo Tollocan, Colonia Residencial Colón, CP, 50120, Toluca, Estado de México, Mexico. Electronic address: lmgomezo@uaemex.mx., García-Medina S; Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo, Ciudad de México, CP, 07700, Mexico., Galar-Martínez M; Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo, Ciudad de México, CP, 07700, Mexico., Jerónimo Juárez JR; Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón Intersección Paseo Tollocan, Colonia Residencial Colón, CP, 50120, Toluca, Estado de México, Mexico., Herrera-Vázquez SE; Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón Intersección Paseo Tollocan, Colonia Residencial Colón, CP, 50120, Toluca, Estado de México, Mexico. |
| المصدر: | Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 May 15; Vol. 349, pp. 123997. Date of Electronic Publication: 2024 Apr 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8804476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6424 (Electronic) Linking ISSN: 02697491 NLM ISO Abbreviation: Environ Pollut Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Barking, Essex, England : Elsevier Applied Science Publishers, c1987- |
| مواضيع طبية MeSH: | Zebrafish* , Liver*/drug effects , Liver*/metabolism , Oxidative Stress*/drug effects , Acetaminophen*/toxicity , Metformin*/pharmacology, Animals ; Dexamethasone/pharmacology ; COVID-19 ; Apoptosis/drug effects ; COVID-19 Drug Treatment ; Superoxide Dismutase/metabolism ; Lipid Peroxidation/drug effects ; Catalase/metabolism ; Water Pollutants, Chemical/toxicity |
| مستخلص: | Current and thorough information on the ecotoxicological consequences of pharmaceuticals is accessible globally. However, there remains a substantial gap in knowledge concerning the potentially toxic effects of COVID-19 used drugs, individually and combined, on aquatic organisms. Given the factors above, our investigation assumes pivotal importance in elucidating whether or not paracetamol, dexamethasone, metformin, and their tertiary mixtures might prompt histological impairment, oxidative stress, and apoptosis in the liver of zebrafish. The findings indicated that all treatments, except paracetamol, augmented the antioxidant activity of superoxide dismutase (SOD) and catalase (CAD), along with elevating the levels of oxidative biomarkers such as lipid peroxidation (LPX), hydroperoxides (HPC), and protein carbonyl content (PCC). Paracetamol prompted a reduction in the activities SOD and CAT and exhibited the most pronounced toxic response when compared to the other treatments. The gene expression patterns paralleled those of oxidative stress, with all treatments demonstrating overexpression of bax, bcl2, and p53. The above suggested a probable apoptotic response in the liver of the fish. Nevertheless, our histological examinations revealed that none of the treatments induced an apoptotic or inflammatory response in the hepatocytes. Instead, the observed tissue alterations encompassed leukocyte infiltration, sinusoidal dilatation, pyknosis, fatty degeneration, diffuse congestion, and vacuolization. In summary, the hepatic toxicity elicited by COVID-19 drugs in zebrafish was less pronounced than anticipated. This attenuation could be attributed to metformin's antioxidant and hormetic effects. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: COVID-19 drugs; Gene expression; Histological damage; Oxidative damage; Zebrafish |
| المشرفين على المادة: | 362O9ITL9D (Acetaminophen) 9100L32L2N (Metformin) 7S5I7G3JQL (Dexamethasone) EC 1.15.1.1 (Superoxide Dismutase) EC 1.11.1.6 (Catalase) 0 (Water Pollutants, Chemical) |
| تواريخ الأحداث: | Date Created: 20240418 Date Completed: 20240504 Latest Revision: 20240504 |
| رمز التحديث: | 20240505 |
| DOI: | 10.1016/j.envpol.2024.123997 |
| PMID: | 38636837 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6424 |
|---|---|
| DOI: | 10.1016/j.envpol.2024.123997 |