Integrated untargeted and targeted testicular metabolomics to reveal the regulated mechanism of Gushudan on the hypothalamic-pituitary-gonadal axis of kidney-yang-deficiency-syndrome rats.

التفاصيل البيبلوغرافية
العنوان:	Integrated untargeted and targeted testicular metabolomics to reveal the regulated mechanism of Gushudan on the hypothalamic-pituitary-gonadal axis of kidney-yang-deficiency-syndrome rats.
المؤلفون:	Lou Y; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China., Liang Q; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China., Xin L; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China., Ren M; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China., Hang Q; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China., Qin F; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China., Xiong Z; School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China.
المصدر:	Biomedical chromatography : BMC [Biomed Chromatogr] 2024 Jul; Vol. 38 (7), pp. e5872. Date of Electronic Publication: 2024 Apr 18.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Wiley Country of Publication: England NLM ID: 8610241 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-0801 (Electronic) Linking ISSN: 02693879 NLM ISO Abbreviation: Biomed Chromatogr Subsets: MEDLINE
أسماء مطبوعة:	Publication: 1990- : Chichester : Wiley Original Publication: London : Heyden & Son, c1986-1990
مواضيع طبية MeSH:	Metabolomics/methods , Yang Deficiency/metabolism , Testis/metabolism , Testis/drug effects , Drugs, Chinese Herbal*/pharmacology, Animals ; Male ; Rats ; Rats, Sprague-Dawley ; Hypothalamo-Hypophyseal System/metabolism ; Hypothalamo-Hypophyseal System/drug effects ; Testosterone/metabolism ; Metabolome/drug effects ; Metabolome/physiology ; Biomarkers/metabolism ; Biomarkers/analysis ; Kidney Diseases/metabolism ; Kidney/metabolism ; Hypothalamic-Pituitary-Gonadal Axis
مستخلص:	Modern studies have shown that neuroendocrine disorders caused by the dysfunction of the hypothalamic-pituitary-gonadal (HPG) axis are one of the important pathogenetic mechanisms of kidney-yang-deficiency-syndrome (KYDS). The preventive effect of Gushudan on KYDS has been reported, but its regulatory mechanisms on the HPG axis have not been elucidated. In this study, we developed an integrated untargeted and targeted metabolomics analysis strategy to investigate the regulatory mechanism of Gushudan on the HPG axis in rats with KYDS. In untargeted metabolomics, we screened 14 potential biomarkers such as glycine, lysine, and glycerol that were significantly associated with the HPG axis. To explore the effect of changes in the levels of potential biomarkers on KYDS, all of them were quantified in targeted metabolomics. With the quantitative results, correlations between potential biomarkers and testosterone, a functional indicator of the HPG axis, were explored. The results showed that oxidative stress, inflammatory response, and energy depletion, induced by metabolic disorders in rats, were responsible for the decrease in testosterone levels. Gushudan improves metabolic disorders and restores testosterone levels, thus restoring HPG axis dysfunction. This finding elucidates the special metabolic characteristics of KYDS and the therapeutic mechanism of Gushudan from a new perspective. (© 2024 John Wiley & Sons Ltd.)
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معلومات مُعتمدة:	82174235 National Natural Science Foundation of China; 81773694 National Natural Science Foundation of China
فهرسة مساهمة:	Keywords: GC–MS; blank matrix; hypothalamic‐pituitary‐gonadal axis; kidney‐yang‐deficiency‐syndrome; metabolomic
المشرفين على المادة:	0 (Drugs, Chinese Herbal) 3XMK78S47O (Testosterone) 0 (Biomarkers)
تواريخ الأحداث:	Date Created: 20240419 Date Completed: 20240618 Latest Revision: 20240618
رمز التحديث:	20240619
DOI:	10.1002/bmc.5872
PMID:	38638009
قاعدة البيانات:	MEDLINE

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تدمد:	1099-0801
DOI:	10.1002/bmc.5872