دورية أكاديمية
High-Throughput DNA-Encoded Libraries Affinity Selection Platform for Binder Identification with Solid Support Protein Immobilization.
| العنوان: | High-Throughput DNA-Encoded Libraries Affinity Selection Platform for Binder Identification with Solid Support Protein Immobilization. |
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| المؤلفون: | Rama-Garda R; Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain.; BioFarma, Universidad de Santiago de Compostela (USC), Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CIMUS), A Coruña, Spain., Domínguez E; Genomic Medicine, Universidad de Santiago de Compostela (USC), Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CIMUS), A Coruña, Spain., Loza MI; BioFarma, Universidad de Santiago de Compostela (USC), Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CIMUS), A Coruña, Spain., Lallena MJ; Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain., de Blas J; Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain., Toledo MÁ; Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain., Haro R; Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain. |
| المصدر: | Assay and drug development technologies [Assay Drug Dev Technol] 2024 May-Jun; Vol. 22 (4), pp. 192-202. Date of Electronic Publication: 2024 Apr 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101151468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8127 (Electronic) Linking ISSN: 1540658X NLM ISO Abbreviation: Assay Drug Dev Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2002- |
| مواضيع طبية MeSH: | High-Throughput Screening Assays* , DNA*/chemistry, Immobilized Proteins/chemistry ; Gene Library ; Ligands |
| مستخلص: | DNA-encoded libraries (DELs) have demonstrated to be one of the most powerful technologies within the ligand identification toolbox, widely used either in academia or biotech and pharma companies. DEL methodology utilizes affinity selection (AS) as the approach to interrogate the protein of interest for the identification of binders. Here we present a high-throughput, fully automated AS platform developed to fulfill industrial standards and compatible with different assay formats to improve the reproducibility of the AS process for DEL binders identification. This platform is flexible enough to virtually set aside all kinds of DELs and AS methods and conditions using immobilized proteins. It bears the two main immobilization methods to support of the proteins of interest: magnetic beads or resin tip columns. A combination of a broad variety of protocol options with a wide range of different experimental conditions can be set up with a throughput of 96 samples at the same time. In addition, small modifications of the protocols provide the platform with the versatility to run not only the routine DEL screens, but also test covalent libraries, the successful immobilization of the proteins of interest, and many other experiments that may be required. This versatile AS platform for DEL can be a powerful instrument for direct application of the technology in academic and industry settings. |
| فهرسة مساهمة: | Keywords: DNA-encoded libraries; affinity selection; automation; quality control |
| المشرفين على المادة: | 9007-49-2 (DNA) 0 (Immobilized Proteins) 0 (Ligands) |
| تواريخ الأحداث: | Date Created: 20240419 Date Completed: 20240618 Latest Revision: 20240618 |
| رمز التحديث: | 20240619 |
| DOI: | 10.1089/adt.2024.010 |
| PMID: | 38638103 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-8127 |
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| DOI: | 10.1089/adt.2024.010 |