دورية أكاديمية
أعرض في EDS

Yeast Crf1p is an activator with different roles in regulation of target genes.

التفاصيل البيبلوغرافية
العنوان: Yeast Crf1p is an activator with different roles in regulation of target genes.
المؤلفون: Kumar S; Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA., Mashkoor M; Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA., Balamurugan P; Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA., Grove A; Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.
المصدر: Yeast (Chichester, England) [Yeast] 2024 Jun; Vol. 41 (6), pp. 379-400. Date of Electronic Publication: 2024 Apr 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: England NLM ID: 8607637 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0061 (Electronic) Linking ISSN: 0749503X NLM ISO Abbreviation: Yeast Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Chichester ; New York : Wiley, c1985-
مواضيع طبية MeSH: Gene Expression Regulation, Fungal* , Mechanistic Target of Rapamycin Complex 1*/metabolism , Mechanistic Target of Rapamycin Complex 1*/genetics , Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae Proteins*/metabolism, High Mobility Group Proteins/genetics ; High Mobility Group Proteins/metabolism ; Ribosomal Proteins/genetics ; Ribosomal Proteins/metabolism ; Trans-Activators
مستخلص: Under stress conditions, ribosome biogenesis is downregulated. This process requires that expression of ribosomal RNA, ribosomal protein, and ribosome biogenesis genes be controlled in a coordinated fashion. The mechanistic Target of Rapamycin Complex 1 (mTORC1) participates in sensing unfavorable conditions to effect the requisite change in gene expression. In Saccharomyces cerevisiae, downregulation of ribosomal protein genes involves dissociation of the activator Ifh1p in a process that depends on Utp22p, a protein that also functions in pre-rRNA processing. Ifh1p has a paralog, Crf1p, which was implicated in communicating mTORC1 inhibition and hence was perceived as a repressor. We focus here on two ribosomal biogenesis genes, encoding Utp22p and the high mobility group protein Hmo1p, both of which are required for communication of mTORC1 inhibition to target genes. Crf1p functions as an activator on these genes as evidenced by reduced mRNA abundance and RNA polymerase II occupancy in a crf1Δ strain. Inhibition of mTORC1 has distinct effects on expression of HMO1 and UTP22; for example, on UTP22, but not on HMO1, the presence of Crf1p promotes the stable depletion of Ifh1p. Our data suggest that Crf1p functions as a weak activator, and that it may be required to prevent re-binding of Ifh1p to some gene promoters after mTORC1 inhibition in situations when Ifh1p is available. We propose that the inclusion of genes encoding proteins required for mTORC1-mediated downregulation of ribosomal protein genes in the same regulatory circuit as the ribosomal protein genes serves to optimize transcriptional responses during mTORC1 inhibition.
(© 2024 John Wiley & Sons Ltd.)
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معلومات مُعتمدة: National Science Foundation
فهرسة مساهمة: Keywords: Hmo1p; Ifh1p; Sfp1p; Utp22p; gene regulation; mTORC1
المشرفين على المادة: 0 (High Mobility Group Proteins)
0 (HMO1 protein, S cerevisiae)
0 (IFH1 protein, S cerevisiae)
EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
0 (Ribosomal Proteins)
0 (Saccharomyces cerevisiae Proteins)
0 (Trans-Activators)
0 (CRF1 protein, S cerevisiae)
تواريخ الأحداث: Date Created: 20240419 Date Completed: 20240610 Latest Revision: 20240617
رمز التحديث: 20240617
DOI: 10.1002/yea.3939
PMID: 38639144
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-0061
DOI:10.1002/yea.3939