دورية أكاديمية
Complete Genomic Assembly of Mauritian Cynomolgus Macaque Killer Ig-like Receptor and Natural Killer Group 2 Haplotypes.
| العنوان: | Complete Genomic Assembly of Mauritian Cynomolgus Macaque Killer Ig-like Receptor and Natural Killer Group 2 Haplotypes. |
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| المؤلفون: | Prall TM; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI., Karl JA; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI., Varghese JM; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI., Baker DA; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI., Minor NR; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI., Raveendran M; Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX., Harris RA; Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX., Rogers J; Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX., Wiseman RW; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI., O'Connor DH; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI. |
| المصدر: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2024 Jun 01; Vol. 212 (11), pp. 1754-1765. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| مواضيع طبية MeSH: | Receptors, KIR*/genetics , Macaca fascicularis*/genetics , Haplotypes*, Animals ; NK Cell Lectin-Like Receptor Subfamily C/genetics ; Genomics/methods ; Genotype |
| مستخلص: | Mauritian-origin cynomolgus macaques (MCMs) serve as a powerful nonhuman primate model in biomedical research due to their unique genetic homogeneity, which simplifies experimental designs. Despite their extensive use, a comprehensive understanding of crucial immune-regulating gene families, particularly killer Ig-like receptors (KIR) and NK group 2 (NKG2), has been hindered by the lack of detailed genomic reference assemblies. In this study, we employ advanced long-read sequencing techniques to completely assemble eight KIR and seven NKG2 genomic haplotypes, providing an extensive insight into the structural and allelic diversity of these immunoregulatory gene clusters. Leveraging these genomic resources, we prototype a strategy for genotyping KIR and NKG2 using short-read, whole-exome capture data, illustrating the potential for cost-effective multilocus genotyping at colony scale. These results mark a significant enhancement for biomedical research in MCMs and underscore the feasibility of broad-scale genetic investigations. (Copyright © 2024 by The American Association of Immunologists, Inc.) |
| معلومات مُعتمدة: | 75N93021C00006 United States AI NIAID NIH HHS; P51 OD011106 United States OD NIH HHS; HHSN272201600007C United States AI NIAID NIH HHS; C06 RR020141 United States RR NCRR NIH HHS; C06 RR015459 United States RR NCRR NIH HHS; P51 RR000167 United States RR NCRR NIH HHS |
| تواريخ الأحداث: | Date Created: 20240419 Date Completed: 20240520 Latest Revision: 20240528 |
| رمز التحديث: | 20240528 |
| مُعرف محوري في PubMed: | PMC11102026 |
| DOI: | 10.4049/jimmunol.2300856 |
| PMID: | 38639635 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1550-6606 |
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| DOI: | 10.4049/jimmunol.2300856 |