دورية أكاديمية
Orostachys malacophylla (pall.) fisch extracts alleviate intestinal inflammation in Drosophila.
| العنوان: | Orostachys malacophylla (pall.) fisch extracts alleviate intestinal inflammation in Drosophila. |
|---|---|
| المؤلفون: | Kim H; College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province, China; College of Life Science, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea. Electronic address: khi1990826@163.com., Xue H; Department of Children's Emergency Medicine, Women's and Children's Hospital Affiliated to Qingdao University, Qingdao, China. Electronic address: xue.hongmei2008@163.com., Li X; College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province, China. Electronic address: xiaoli@nefu.edu.cn., Yue G; Department of Basic Medical, Shenyang Medical College, Shenyang, China. Electronic address: la062142@163.com., Zhu J; Department of Basic Medical, Shenyang Medical College, Shenyang, China. Electronic address: zgzjnbyzzjh@163.com., Eh T; College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province, China; College of Life Science, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea. Electronic address: etj527@163.com., Wang S; Analysis and Test Center, Yanbian University, Yanji 133002, Jilin Province, PR China. Electronic address: shwang@ybu.edu.cn., Jin LH; College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province, China. Electronic address: lhjin2000@hotmail.com. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2024 Aug 10; Vol. 330, pp. 118215. Date of Electronic Publication: 2024 Apr 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Plant Extracts*/pharmacology , Plant Extracts*/chemistry , Reactive Oxygen Species*/metabolism , Dextran Sulfate* , Pectobacterium carotovorum*/drug effects, Animals ; Crassulaceae/chemistry ; Intestines/drug effects ; Intestines/pathology ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/isolation & purification ; Drosophila melanogaster/drug effects ; Disease Models, Animal ; Drosophila ; Network Pharmacology ; Inflammation/drug therapy ; Antimicrobial Cationic Peptides/pharmacology |
| مستخلص: | Ethnopharmacological Relevance: Orostachys malacophylla (Pall.) Fisch (O. malacophylla) is a succulent herbaceous plant that is the Orostachys genus of Crassulaceae family. O. malacophylla has been widely used as a traditional Chinese medicine with antioxidant, anti-inflammatory, anti-febrile, antidote, anti-Toxoplasma gondii properties. However, the biological function of alleviating intestinal inflammation and key bioactive compounds were still unknown. Aim of the Study: We used a Drosophila model to study the protective effects and bioactive compounds of O. malacophylla water extract (OMWE) and butanol extract (OMBE) on intestinal inflammation. Materials and Methods: Drosophila intestinal inflammation was induced by oral invasion of dextran sodium sulfate (DSS) or Erwinia carotovora carotovora 15 (Ecc15). We revealed the protective effects of two extracts by determining intestinal reactive oxygen species (ROS) and antimicrobial peptide (AMP) levels and intestinal integrity, and using network pharmacology analysis to identify bioactive compounds. Results: We demonstrated that both OMWE and OMBE could ameliorate the detrimental effects of DSS, including a decreased survival rate, elevated ROS levels, increased cell death, excessive proliferation of ISCs, acid-base imbalance, and disruption of intestinal integrity. Moreover, the overabundance of lipid droplets (LDs) and AMPs by Ecc15 infection is mitigated by these extracts, thereby enhancing the flies' resistance to adverse stimuli. In addition, we used widely targeted metabolomics and network pharmacology analysis to identify bioactive compounds associated with IBD healing that are present in OMWE and OMBE. Conclusions: In summary, our research indicates that OMWE and OMBE significantly mitigate intestinal inflammation and have the potential to be effective therapeutic agents for IBD in humans. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Drosophila; Inflammatory bowel disease; Intestinal homeostasis; Network pharmacology; Orostachys malacophylla (pall.) fisch |
| المشرفين على المادة: | 0 (Plant Extracts) 0 (Reactive Oxygen Species) 9042-14-2 (Dextran Sulfate) 0 (Anti-Inflammatory Agents) 0 (Antimicrobial Cationic Peptides) |
| تواريخ الأحداث: | Date Created: 20240419 Date Completed: 20240510 Latest Revision: 20240510 |
| رمز التحديث: | 20240511 |
| DOI: | 10.1016/j.jep.2024.118215 |
| PMID: | 38641073 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
|---|---|
| DOI: | 10.1016/j.jep.2024.118215 |