دورية أكاديمية
Nucleoside Diphosphate Kinase FgNdpk Is Required for DON Production and Pathogenicity by Regulating the Growth and Toxisome Formation of Fusarium graminearum .
| العنوان: | Nucleoside Diphosphate Kinase FgNdpk Is Required for DON Production and Pathogenicity by Regulating the Growth and Toxisome Formation of Fusarium graminearum . |
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| المؤلفون: | Mao X; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou 350108, China.; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Li L; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Abubakar YS; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.; Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria 810281, Nigeria., Li Y; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou 350108, China.; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Luo Z; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Chen M; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou 350108, China., Zheng W; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Wang Z; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou 350108, China.; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Zheng H; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou 350108, China. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2024 May 01; Vol. 72 (17), pp. 9637-9646. Date of Electronic Publication: 2024 Apr 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Fusarium*/genetics , Fusarium*/enzymology , Fusarium*/metabolism , Fusarium*/growth & development , Fungal Proteins*/genetics , Fungal Proteins*/metabolism , Trichothecenes*/metabolism , Plant Diseases*/microbiology , Spores, Fungal*/growth & development , Spores, Fungal*/genetics , Nucleoside-Diphosphate Kinase*/genetics , Nucleoside-Diphosphate Kinase*/metabolism, Gene Expression Regulation, Fungal ; Virulence ; Triticum/microbiology |
| مستخلص: | Nucleoside diphosphate kinases (NDPKs) are nucleotide metabolism enzymes that play different physiological functions in different species. However, the roles of NDPK in phytopathogen and mycotoxin production are not well understood. In this study, we showed that Fusarium graminearum FgNdpk is important for vegetative growth, conidiation, sexual development, and pathogenicity. Furthermore, FgNdpk is required for deoxynivalenol (DON) production; deletion of FgNDPK downregulates the expression of DON biosynthesis genes and disrupts the formation of FgTri4-GFP-labeled toxisomes, while overexpression of FgNDPK significantly increases DON production. Interestingly, FgNdpk colocalizes with the DON biosynthesis proteins FgTri1 and FgTri4 in the toxisome, and coimmunoprecipitation (Co-IP) assays show that FgNdpk associates with FgTri1 and FgTri4 in vivo and regulates their localizations and expressions, respectively. Taken together, these data demonstrate that FgNdpk is important for vegetative growth, conidiation, and pathogenicity and acts as a key protein that regulates toxisome formation and DON biosynthesis in F. graminearum . |
| فهرسة مساهمة: | Keywords: FgNdpk; Fusarium graminearum; deoxynivalenol; nucleoside diphosphate kinase; pathogenicity; toxisome |
| المشرفين على المادة: | 0 (Fungal Proteins) 0 (Trichothecenes) JT37HYP23V (deoxynivalenol) EC 2.7.4.6 (Nucleoside-Diphosphate Kinase) |
| SCR Organism: | Fusarium graminearum |
| تواريخ الأحداث: | Date Created: 20240420 Date Completed: 20240501 Latest Revision: 20240501 |
| رمز التحديث: | 20240501 |
| DOI: | 10.1021/acs.jafc.4c00593 |
| PMID: | 38642053 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.4c00593 |