دورية أكاديمية
Effect of micro- and nanoplastic particles on human macrophages.
| العنوان: | Effect of micro- and nanoplastic particles on human macrophages. |
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| المؤلفون: | Adler MY; Department of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany., Issoual I; Department of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany; Chair of Machine Learning and Data Analytics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany., Rückert M; Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany., Deloch L; Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany., Meier C; Institute of Anatomy and Cell Biology, Saarland University, Homburg/Saar, Germany., Tschernig T; Institute of Anatomy and Cell Biology, Saarland University, Homburg/Saar, Germany., Alexiou C; Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany., Pfister F; Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany., Ramsperger AF; Animal Ecology I and Bay CEER, University of Bayreuth, Bayreuth, Germany., Laforsch C; Animal Ecology I and Bay CEER, University of Bayreuth, Bayreuth, Germany., Gaipl US; Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany., Jüngert K; Department of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany. Electronic address: katharina.juengert@fau.de., Paulsen F; Department of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany. |
| المصدر: | Journal of hazardous materials [J Hazard Mater] 2024 Jun 05; Vol. 471, pp. 134253. Date of Electronic Publication: 2024 Apr 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, |
| مواضيع طبية MeSH: | Macrophages*/drug effects , Macrophages*/metabolism , Reactive Oxygen Species*/metabolism , Polystyrenes*/chemistry , Polystyrenes*/toxicity , Microplastics*/toxicity, Humans ; THP-1 Cells ; Leukocytes, Mononuclear/drug effects ; Nanoparticles/toxicity ; Nanoparticles/chemistry ; Cell Survival/drug effects ; Microscopy, Electron, Transmission ; Particle Size |
| مستخلص: | Micro- and nanoplastics (MNPs) are ubiquitous in the environment, resulting in the uptake of MNPs by a variety of organisms, including humans, leading to particle-cell interaction. Human macrophages derived from THP-1 cell lines take up Polystyrene (PS), a widespread plastic. The question therefore arises whether primary human macrophages also take up PS micro- and nanobeads (MNBs) and how they react to this stimulation. Major aim of this study is to visualize this uptake and to validate the isolation of macrophages from peripheral blood mononuclear cells (PBMCs) to assess the impact of MNPs on human macrophages. Uptake of macrophages from THP-1 cell lines and PBMCs was examined by transmission electron microscopy (TEM), scanning electron microscopy and live cell imaging. In addition, the reaction of the macrophages was analyzed in terms of metabolic activity, cytotoxicity, production of reactive oxygen species (ROS) and macrophage polarization. This study is the first to visualize PS MNBs in primary human cells using TEM and live cell imaging. Metabolic activity was size- and concentration-dependent, necrosis and ROS were increased. The methods demonstrated in this study outline an approach to assess the influence of MNP exposure on human macrophages and help investigating the consequences of worldwide plastic pollution. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Competing interests The authors declare no competing interests regarding the publication of this article. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Live cell imaging; MNPs; Macrophages; Polystyrene beads; Transmission electron microscopy |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) 0 (Polystyrenes) 0 (Microplastics) |
| تواريخ الأحداث: | Date Created: 20240420 Date Completed: 20240511 Latest Revision: 20240511 |
| رمز التحديث: | 20240511 |
| DOI: | 10.1016/j.jhazmat.2024.134253 |
| PMID: | 38642497 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3336 |
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| DOI: | 10.1016/j.jhazmat.2024.134253 |