دورية أكاديمية
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A live attenuated influenza B virus vaccine expressing RBD elicits protective immunity against SARS-CoV-2 in mice.

التفاصيل البيبلوغرافية
العنوان: A live attenuated influenza B virus vaccine expressing RBD elicits protective immunity against SARS-CoV-2 in mice.
المؤلفون: Wang Z; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China; College of Animal Science and Technology, College of Veterinary and Medicine, Jilin Agricultural University, Changchun, China., Sun W; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Li D; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China; College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China., Sun Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China; Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, PR China., Zhu M; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China; Henan International Joint Laboratory for Nuclear Protein Regulation, Henan University, School of Basic Medical Sciences, Kaifeng, China., Wang W; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China; Key Laboratory of Animal Resistant Biology of Shandong, College of Life Sciences, Shandong Normal University, Jinan, China., Zhang Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China; Key Laboratory of Animal Resistant Biology of Shandong, College of Life Sciences, Shandong Normal University, Jinan, China., Li E; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Yan F; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Wang T; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Feng N; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Yang S; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Xia X; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China., Gao Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China. Electronic address: yuwei0901@outlook.com.
المصدر: Virus research [Virus Res] 2024 Jul; Vol. 345, pp. 199378. Date of Electronic Publication: 2024 Apr 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8410979 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7492 (Electronic) Linking ISSN: 01681702 NLM ISO Abbreviation: Virus Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science, c1984-
مواضيع طبية MeSH: Influenza B virus*/immunology , Influenza B virus*/genetics , Antibodies, Viral*/blood , Antibodies, Viral*/immunology , SARS-CoV-2*/immunology , SARS-CoV-2*/genetics , COVID-19*/prevention & control , COVID-19*/immunology , Vaccines, Attenuated*/immunology , Vaccines, Attenuated*/administration & dosage , Vaccines, Attenuated*/genetics , COVID-19 Vaccines*/immunology , COVID-19 Vaccines*/administration & dosage , Mice, Inbred BALB C*, Animals ; Mice ; Female ; Administration, Intranasal ; Humans ; Spike Glycoprotein, Coronavirus/immunology ; Spike Glycoprotein, Coronavirus/genetics ; Influenza Vaccines/immunology ; Influenza Vaccines/administration & dosage ; Influenza Vaccines/genetics ; Immunoglobulin A/blood ; Disease Models, Animal ; Immunoglobulin G/blood ; Viral Load ; Antibodies, Neutralizing/blood ; Antibodies, Neutralizing/immunology
مستخلص: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a significant threat to human health globally. It is crucial to develop a vaccine to reduce the effect of the virus on public health, economy, and society and regulate the transmission of SARS-CoV-2. Influenza B virus (IBV) can be used as a vector that does not rely on the current circulating influenza A strains. In this study, we constructed an IBV-based vector vaccine by inserting a receptor-binding domain (RBD) into a non-structural protein 1 (NS1)-truncated gene (rIBV-NS110-RBD). Subsequently, we assessed its safety, immunogenicity, and protective efficacy against SARS-CoV-2 in mice, and observed that it was safe in a mouse model. Intranasal administration of a recombinant rIBV-NS110-RBD vaccine induced high levels of SARS-CoV-2-specific IgA and IgG antibodies and T cell-mediated immunity in mice. Administering two doses of the intranasal rIBV-NS110-RBD vaccine significantly reduced the viral load and lung damage in mice. This novel IBV-based vaccine offers a novel approach for controlling the SARS-CoV-2 pandemic.
Competing Interests: Declaration of competing interest The authors declare that this study was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.
(Copyright © 2024. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Influenza virus-vector vaccine; Intranasal vaccination; Mice; SARS-CoV-2; Spike RBD
المشرفين على المادة: 0 (Antibodies, Viral)
0 (Vaccines, Attenuated)
0 (COVID-19 Vaccines)
0 (Spike Glycoprotein, Coronavirus)
0 (Influenza Vaccines)
0 (Immunoglobulin A)
0 (Immunoglobulin G)
0 (Antibodies, Neutralizing)
تواريخ الأحداث: Date Created: 20240421 Date Completed: 20240527 Latest Revision: 20240604
رمز التحديث: 20240604
مُعرف محوري في PubMed: PMC11059473
DOI: 10.1016/j.virusres.2024.199378
PMID: 38643857
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-7492
DOI:10.1016/j.virusres.2024.199378