دورية أكاديمية
Identification of third stage larvae of strongyles and molecular diagnosis of Strongylus vulgaris in the feces of Thoroughbred horses kept in training centers in Rio de Janeiro, Brazil.
| العنوان: | Identification of third stage larvae of strongyles and molecular diagnosis of Strongylus vulgaris in the feces of Thoroughbred horses kept in training centers in Rio de Janeiro, Brazil. |
|---|---|
| المؤلفون: | Martins AV; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil; Laboratório de Parasitologia e Doenças Parasitárias, Faculdade de Medicina Veterinária, Centro Universitário Serra dos Órgãos, Teresópolis, Rio de Janeiro, Brazil., Corrêa LL; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Ribeiro MS; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., de Lima Coelho A; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Lobão LF; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Palmer JPS; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Knackfuss FB; Zootecnia e Estatística, Universidade do Grande Rio, Duque de Caxias, Rio de Janeiro, Brazil., Molento MB; Laboratório de Parasitologia Clínica Veterinária, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., da Silva Barbosa A; Laboratório de Bioagentes Ambientais, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil; Laboratório de Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil. Electronic address: alynnedsb@gmail.com. |
| المصدر: | Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2024 May; Vol. 50, pp. 101019. Date of Electronic Publication: 2024 Apr 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101680410 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-9390 (Electronic) Linking ISSN: 24059390 NLM ISO Abbreviation: Vet Parasitol Reg Stud Reports Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, [2015]- |
| مواضيع طبية MeSH: | Feces*/parasitology , Strongylus*/isolation & purification , Larva* , Strongyle Infections, Equine*/diagnosis , Strongyle Infections, Equine*/parasitology, Animals ; Horses ; Brazil ; Male ; Female ; Horse Diseases/diagnosis ; Horse Diseases/parasitology ; Parasite Egg Count/veterinary ; Polymerase Chain Reaction/veterinary ; DNA, Helminth/analysis ; Anthelmintics/therapeutic use |
| مستخلص: | The aims of the present study were to identify strongyles in the feces of Thoroughbred horses based on larval morphology; to detect Strongylus vulgaris using molecular diagnosis and compare results to those of feces culture; and to determine the association between the presence of S. vulgaris with corresponding animal information (age range, gender, and anthelmintic use). Feces of horses kept in six Training Centers in Rio de Janeiro State, that showed the presence of ≥500 eggs per gram of feces (EPG) were subjected to strongyle identification. Of the 520 fecal samples collected, 35 had an EPG ≥ 500. After fecal culture for L3 larvae identification, DNA was extracted, subjected to PCR to amplify the ITS2 region DNA fragment of S. vulgaris, and sequenced. A total of 3500 larvae were analyzed. Most were classified as small strong (99.7%), with an emphasis on the type A subfamily of Cyathostominae. Forms of S. vulgaris only corresponded to 0.2%. In all, 25 samples showed amplified S. vulgaris DNA products and 11 showed nucleotide sequences with high sequence identity. Fecal culture and PCR results showed poor agreement (kappa = 0.105) for S. vulgaris diagnosis. Age, gender, anthelmintic use, and anthelmintic administration interval were not statistically significant. The present study showed the presence of S. vulgaris in the feces of horses kept in Rio de Janeiro Training Centers, mainly seen via PCR, which has emerged as the most effective tool for diagnosis. This study made it possible to identify strongyles that infect horses in the region, emphasizing upon the necessity for constant monitoring of the animals. Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Fecal culture; Molecular diagnosis; Small strongyles; Strongylus vulgaris; Thoroughbred horses |
| المشرفين على المادة: | 0 (DNA, Helminth) 0 (Anthelmintics) |
| تواريخ الأحداث: | Date Created: 20240421 Date Completed: 20240423 Latest Revision: 20240502 |
| رمز التحديث: | 20240502 |
| DOI: | 10.1016/j.vprsr.2024.101019 |
| PMID: | 38644042 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2405-9390 |
|---|---|
| DOI: | 10.1016/j.vprsr.2024.101019 |