دورية أكاديمية
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A novel mouse model carrying a gene trap insertion into the Hmgxb4 gene locus to examine Hmgxb4 expression in vivo.

التفاصيل البيبلوغرافية
العنوان: A novel mouse model carrying a gene trap insertion into the Hmgxb4 gene locus to examine Hmgxb4 expression in vivo.
المؤلفون: Wang L; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China.; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA., He X; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA., Hu G; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA., Liu J; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.; Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China., Kang X; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.; Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China., Yu L; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.; Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China., Dong K; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA., Zhao J; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA., Zhang A; Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.; Training Center, Guangxi Medical College, Nanning, China., Zhang W; Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China., Brands MW; Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA., Su H; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.; Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, Georgia, USA., Zheng Z; Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China., Zhou J; Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
المصدر: Physiological reports [Physiol Rep] 2024 Apr; Vol. 12 (8), pp. e16014.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013-
مواضيع طبية MeSH: High Mobility Group Proteins*/genetics , High Mobility Group Proteins*/metabolism , Mice, Inbred C57BL*, Animals ; Male ; Mice ; beta-Galactosidase/metabolism ; beta-Galactosidase/genetics ; Lac Operon/genetics ; Mice, Transgenic ; Transcription Factors/genetics ; Transcription Factors/metabolism
مستخلص: HMG (high mobility group) proteins are a diverse family of nonhistone chromosomal proteins that interact with DNA and a wide range of transcriptional regulators to regulate the structural architecture of DNA. HMGXB4 (also known as HMG2L1) is an HMG protein family member that contains a single HMG box domain. Our previous studies have demonstrated that HMGXB4 suppresses smooth muscle differentiation and exacerbates endotoxemia by promoting a systemic inflammatory response in mice. However, the expression of Hmgxb4 in vivo has not fully examined. Herein, we generated a mouse model that harbors a gene trap in the form of a lacZ gene insertion into the Hmgxb4 gene. This mouse enables the visualization of endogenous HMGXB4 expression in different tissues via staining for the β-galactosidase activity of LacZ which is under the control of the endogenous Hmgxb4 gene promoter. We found that HMGXB4 is widely expressed in mouse tissues and is a nuclear protein. Furthermore, the Hmgxb4 gene trap mice exhibit normal cardiac function and blood pressure. Measurement of β-galactosidase activity in the Hmgxb4 gene trap mice demonstrated that the arterial injury significantly induces Hmgxb4 expression. In summary, the Hmgxb4 gene trap reporter mouse described here provides a valuable tool to examine the expression level of endogenous Hmgxb4 in both physiological and pathological settings in vivo.
(© 2024 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)
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معلومات مُعتمدة: R01 HL165205 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: Hmgxb4; arterial injury; gene expression; gene trap; β‐galactosidase activity
المشرفين على المادة: EC 3.2.1.23 (beta-Galactosidase)
0 (High Mobility Group Proteins)
0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20240421 Date Completed: 20240423 Latest Revision: 20240510
رمز التحديث: 20240511
مُعرف محوري في PubMed: PMC11033291
DOI: 10.14814/phy2.16014
PMID: 38644513
قاعدة البيانات: MEDLINE
الوصف
تدمد:2051-817X
DOI:10.14814/phy2.16014