دورية أكاديمية
YiQi GuBen capsule alleviates OVA-induced asthma through improving mitochondrial dysfunction.
| العنوان: | YiQi GuBen capsule alleviates OVA-induced asthma through improving mitochondrial dysfunction. |
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| المؤلفون: | Ren X; College of Traditional Chinese Medicine, Changchun University of Chinese Medical, Changchun, China., Kong Y; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Yu H; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Dong A; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Wang Y; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Wei L; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Song Y; College of Traditional Chinese Medicine, Changchun University of Chinese Medical, Changchun, China., Wang Z; College of Traditional Chinese Medicine, Changchun University of Chinese Medical, Changchun, China., Wang L; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Guo Y; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China., Sun L; Department of Pediatrics, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China. |
| المصدر: | The Journal of asthma : official journal of the Association for the Care of Asthma [J Asthma] 2024 Jul; Vol. 61 (7), pp. 725-735. Date of Electronic Publication: 2024 Apr 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 8106454 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-4303 (Electronic) Linking ISSN: 02770903 NLM ISO Abbreviation: J Asthma Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: [Ossining, N.Y. : Asthma Publications Society, c1981- |
| مواضيع طبية MeSH: | Asthma*/drug therapy , Asthma*/pathology , Drugs, Chinese Herbal*/pharmacology , Ovalbumin* , Mitochondria*/drug effects , Mitochondria*/metabolism , Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha*/metabolism , Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha*/genetics , Lung*/drug effects , Lung*/pathology , Reactive Oxygen Species*/metabolism , DNA, Mitochondrial*/drug effects, Animals ; Mice ; Disease Models, Animal ; Mice, Inbred BALB C ; Membrane Potential, Mitochondrial/drug effects ; Female ; Dexamethasone/pharmacology ; Transcription Factors/metabolism ; Transcription Factors/genetics ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Mitochondrial Proteins/genetics ; Mitochondrial Proteins/metabolism ; Capsules ; High Mobility Group Proteins |
| مستخلص: | Objective: This study aims to explore the effect of YiQi GuBen capsule on improving mitochondrial dysfunction in an animal model of asthma. Methods: The mice ( n = 8) were divided into four groups including control (NC), ovalbumin (OVA), dexamethasone (OVA + DEX), and YiQi GuBen (OVA + YQGB) groups. Firstly, we established an OVA-induced mouse asthma model except for the NC group, which then were treated with dexamethasone and YiQi GuBen capsule. Subsequently, HE staining and Masson staining were used for pathological analysis of mice lung tissues. Next, we used transmission electron microscopy (TEM) to observe the effect of the Yiqi Guben capsule on the ultrastructure of mitochondria. Flow cytometry was used to analyze the ROS level, membrane potential, and the number of mitochondria in lung tissue. Moreover, we analyzed the copy number of mitochondrial DNA (mtDNA) and the expression levels of activator peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and mitochondrial transcription factor A (TFAM). Results: The results of the pathological analysis showed that after treatment with the YiQi GuBen capsule, the lung tissue damage was significantly reduced. In addition, we observed that the ultrastructural damage of mitochondria was improved. Flow cytometry proved that after treatment with the YiQi GuBen capsule, the level of ROS in the mitochondria was effectively reduced, while the mitochondrial membrane potential decreased and the number increased significantly. Moreover, we found that the copy number of mtDNA was significantly increased and the expression levels of PGC-1α and TFAM were significantly upgraded. Conclusion: This study suggests YiQi GuBen capsule can effectively improve mitochondrial dysfunction in the OVA-induced mouse model. |
| فهرسة مساهمة: | Keywords: YiQi GuBen capsule; allergic asthma; mitochondrial dysfunction; ovalbumin |
| المشرفين على المادة: | 0 (Drugs, Chinese Herbal) 9006-59-1 (Ovalbumin) 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha) 0 (Reactive Oxygen Species) 0 (DNA, Mitochondrial) 0 (Ppargc1a protein, mouse) 7S5I7G3JQL (Dexamethasone) 0 (Transcription Factors) 0 (DNA-Binding Proteins) 0 (Tfam protein, mouse) 0 (Mitochondrial Proteins) 0 (Capsules) 0 (High Mobility Group Proteins) |
| تواريخ الأحداث: | Date Created: 20240422 Date Completed: 20240611 Latest Revision: 20240611 |
| رمز التحديث: | 20240611 |
| DOI: | 10.1080/02770903.2024.2303755 |
| PMID: | 38647486 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1532-4303 |
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| DOI: | 10.1080/02770903.2024.2303755 |