دورية أكاديمية
Pirin Promotes the Progression of Non-Small-Cell Lung Cancer by Increasing ODC1 to Suppress Autophagy.
| العنوان: | Pirin Promotes the Progression of Non-Small-Cell Lung Cancer by Increasing ODC1 to Suppress Autophagy. |
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| المؤلفون: | Li YP; Research Laboratory of Zhuang & Yao Medicine, Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530201, China., Huang ZJ; MOE Key Laboratory of Tumor Molecular Biology and State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology, Jinan University, Guangzhou 510632, China., He QK; Medical College of Guangxi University, Nanning, 530004 China., Li YX; Medical College of Guangxi University, Nanning, 530004 China., Zhao XP; Research Laboratory of Zhuang & Yao Medicine, Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530201, China., Ma ZQ; Medical College of Guangxi University, Nanning, 530004 China., Qin MJ; Medical Experimental Center, The First People's Hospital of Nanning, Nanning Institute of Respiratory Diseases, Nanning, 530022 China., Chen AW; MOE Key Laboratory of Tumor Molecular Biology and State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology, Jinan University, Guangzhou 510632, China., Wei Q; Medical Experimental Center, The First People's Hospital of Nanning, Nanning Institute of Respiratory Diseases, Nanning, 530022 China., Wang Y; MOE Key Laboratory of Tumor Molecular Biology and State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology, Jinan University, Guangzhou 510632, China., Lu CH; Medical Experimental Center, The First People's Hospital of Nanning, Nanning Institute of Respiratory Diseases, Nanning, 530022 China. |
| المصدر: | Journal of proteome research [J Proteome Res] 2024 May 03; Vol. 23 (5), pp. 1713-1724. Date of Electronic Publication: 2024 Apr 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: | Autophagy*/genetics , Carcinoma, Non-Small-Cell Lung*/genetics , Carcinoma, Non-Small-Cell Lung*/pathology , Carcinoma, Non-Small-Cell Lung*/metabolism , Cell Proliferation*/genetics , Disease Progression* , Lung Neoplasms*/genetics , Lung Neoplasms*/pathology , Lung Neoplasms*/metabolism , Ornithine Decarboxylase*/metabolism , Ornithine Decarboxylase*/genetics, Female ; Humans ; Male ; A549 Cells ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Prognosis ; Up-Regulation |
| مستخلص: | Non-small-cell lung cancer (NSCLC), a common malignant tumor, requires deeper pathogenesis investigation. Autophagy is an evolutionarily conserved lysosomal degradation process that is frequently blocked during cancer progression. It is an urgent need to determine the novel autophagy-associated regulators in NSCLC. Here, we found that pirin was upregulated in NSCLC, and its expression was positively correlated with poor prognosis. Overexpression of pirin inhibited autophagy and promoted NSCLC proliferation. We then performed data-independent acquisition-based quantitative proteomics to identify the differentially expressed proteins (DEPs) in pirin-overexpression (OE) or pirin-knockdown (KD) cells. Among the pirin-regulated DEPs, ornithine decarboxylase 1 (ODC1) was downregulated in pirin-KD cells while upregulated along with pirin overexpression. ODC1 depletion reversed the pirin-induced autophagy inhibition and pro-proliferation effect in A549 and H460 cells. Immunohistochemistry showed that ODC1 was highly expressed in NSCLC cancer tissues and positively related with pirin. Notably, NSCLC patients with pirin high /ODC1 high had a higher risk in terms of overall survival. In summary, we identified pirin and ODC1 as a novel cluster of prognostic biomarkers for NSCLC and highlighted the potential oncogenic role of the pirin/ODC1/autophagy axis in this cancer type. Targeting this pathway represents a possible therapeutic approach to treat NSCLC. |
| فهرسة مساهمة: | Keywords: ODC1; autophagy; cell proliferation; non-small-cell lung cancer; pirin |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) EC 4.1.1.17 (Ornithine Decarboxylase) 0 (SLC25A21 protein, human) |
| تواريخ الأحداث: | Date Created: 20240422 Date Completed: 20240503 Latest Revision: 20240523 |
| رمز التحديث: | 20240523 |
| DOI: | 10.1021/acs.jproteome.3c00871 |
| PMID: | 38648079 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1535-3907 |
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| DOI: | 10.1021/acs.jproteome.3c00871 |