دورية أكاديمية
Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification.
| العنوان: | Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification. |
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| المؤلفون: | Tian R; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China., Sun J; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China., Ye Y; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China., Lu X; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China., Wang W; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China., Sun X; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2024 May 01; Vol. 72 (17), pp. 10046-10054. Date of Electronic Publication: 2024 Apr 22. |
| نوع المنشور: | Journal Article; Evaluation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Nucleic Acid Amplification Techniques*/methods , Aptamers, Nucleotide*/chemistry , Limit of Detection* , Biosensing Techniques*/methods , Biosensing Techniques*/instrumentation, Alpha-Amanitin/chemistry ; Nanostructures/chemistry ; DNA/chemistry ; Agaricales/chemistry |
| مستخلص: | Poisonous mushrooms containing α-amatoxin can be lethal, making it imperative to develop a rapid and sensitive detection method for α-amatoxin. Utilizing the DNA tetrahedral structure as its foundation, the aptamer allows controlled density and orientation. Consequently, we designed aptamer tetrahedral functionalized magnetic beads that specifically target α-amanitin to release complementary DNA (C-DNA) strands. These strands were then employed as primers to initiate rolling circle amplification (RCA) with fluorescent dyes. The combination of SYBR Green I detection probes facilitated the amplification of the detection signal, enhancing the detection sensitivity of the aptasensor. The calculated detection limit was determined to be 3 ng/mL, a magnitude lower than that of other aptasensors by 2 orders of magnitude. The aptasensor integrates the advantages of high sensitivity and specificity, offering a simple and reliable rapid detection method for α-amanitin analysis. |
| فهرسة مساهمة: | Keywords: DNA tetrahedron; aptamer; food safety; rolling circle amplification; α-amanitin |
| المشرفين على المادة: | 0 (Aptamers, Nucleotide) 0 (Alpha-Amanitin) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20240422 Date Completed: 20240501 Latest Revision: 20240501 |
| رمز التحديث: | 20240501 |
| DOI: | 10.1021/acs.jafc.4c00797 |
| PMID: | 38648503 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.4c00797 |