دورية أكاديمية
Sheep abortions associated with Neospora caninum and Toxoplasma gondii infections in multiple flocks from Southern Brazil.
| العنوان: | Sheep abortions associated with Neospora caninum and Toxoplasma gondii infections in multiple flocks from Southern Brazil. |
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| المؤلفون: | Withoeft JA; Laboratório de Patologia Animal, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brasil., Marian L; Laboratório de Patologia Animal, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brasil., da Costa LS; Laboratório de Patologia Animal, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brasil., Fernandes FD; Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Prédio 63D, Bairro Camobi, Santa Maria, Rio Grande do Sul, 97105-900, Brasil., Vogel FSF; Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Prédio 63D, Bairro Camobi, Santa Maria, Rio Grande do Sul, 97105-900, Brasil., das Neves GB; Laboratório de Bioquímica de Hemoparasitas e Vetores, CAV/UDESC, Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brasil., Miletti LC; Laboratório de Bioquímica de Hemoparasitas e Vetores, CAV/UDESC, Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brasil., Casagrande RA; Laboratório de Patologia Animal, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brasil. renata.casagrande@udesc.br. |
| المصدر: | Veterinary research communications [Vet Res Commun] 2024 Aug; Vol. 48 (4), pp. 2699-2705. Date of Electronic Publication: 2024 Apr 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Switzerland NLM ID: 8100520 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7446 (Electronic) Linking ISSN: 01657380 NLM ISO Abbreviation: Vet Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [Cham] : Springer Original Publication: [Amsterdam] : Elsevier, 1980- |
| مواضيع طبية MeSH: | Toxoplasmosis, Animal*/epidemiology , Toxoplasmosis, Animal*/parasitology , Neospora*/genetics , Neospora*/isolation & purification , Coccidiosis*/veterinary , Coccidiosis*/parasitology , Coccidiosis*/epidemiology , Abortion, Veterinary*/parasitology , Abortion, Veterinary*/epidemiology , Toxoplasma*/genetics , Toxoplasma*/isolation & purification , Sheep Diseases*/parasitology , Sheep Diseases*/epidemiology , Sheep Diseases*/virology, Animals ; Brazil/epidemiology ; Sheep ; Female ; Pregnancy |
| مستخلص: | Neosporosis and toxoplasmosis are important parasitic causes of abortions in small ruminants. This study verified the occurrence of these diseases in sheep fetuses from Santa Catarina State, Southern Brazil from 2015 to 2022. Sheep fetuses were necropsied with organ sampling for histopathology, and polymerase chain reaction (PCR) for Neospora caninum and Toxoplasma gondii using the Nc5 and SAG2 targets, respectively, in frozen brain tissue. Microbiological culture and RT-PCR for Pestivirus were conducted to discard other abortion causes. One positive fetus for toxoplasmosis was genotyped using multiplex multilocus nested PCR-RFLP (Mn-PCR-RFLP) with ten genetic markers. Fifty-five sheep fetuses were evaluated, with 10 (18.2%) cases of neosporosis and 7 (12.7%) cases of toxoplasmosis, comprising six and four flocks, respectively. Macroscopically, neosporosis abortions exhibited fetal mummification, maceration, and arthrogryposis. Toxoplasmosis abortions showed fetal mummification and maceration. The neosporosis abortions included lymphoplasmacytic myositis (70%; 7/10) and myocarditis (60%; 6/10), in addition to necrotizing encephalitis and gliosis (50%; 5/10). Toxoplasmosis abortions included lymphoplasmacytic necrotizing encephalitis (71.4%; 5/7), lymphoplasmacytic myositis (42.8%, 3/7), and myocarditis (14.3%; 1/7). Through PCR, N. caninum and T. gondii were detected in 6 (60%) and 5 (71.4%) fetuses, respectively. In one fetus, T. gondii genotyping was conducted, which was characterized as atypical genotype ToxoDB #98. All of the cases were negative for Pestivirus and bacterial agents. This study establishes the occurrence of these diseases as causes of abortions, malformations, mummification, and fetal maceration in sheep, with the characterization of an atypical T. gondii genotype in one of the fetuses. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.) |
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| معلومات مُعتمدة: | FAPESC, 05/2018 - No.: 2019TR702 Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina; PROAP/CAPES, Finance code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| فهرسة مساهمة: | Keywords: Abortion; Apicomplexa; Lambing; Protozoan; Reproductive loss; Sheep |
| تواريخ الأحداث: | Date Created: 20240423 Date Completed: 20240809 Latest Revision: 20240809 |
| رمز التحديث: | 20240809 |
| DOI: | 10.1007/s11259-024-10390-4 |
| PMID: | 38653939 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-7446 |
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| DOI: | 10.1007/s11259-024-10390-4 |