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Cascading Detection of Hydrogen Sulfide and N -Acetyltransferase 2 in Hepatocellular Carcinoma Cells Using a Two-Photon Fluorescent Probe.

التفاصيل البيبلوغرافية
العنوان: Cascading Detection of Hydrogen Sulfide and N -Acetyltransferase 2 in Hepatocellular Carcinoma Cells Using a Two-Photon Fluorescent Probe.
المؤلفون: Liu Y; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China.; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China., Wang X; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China., Li Z; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China., Chen L; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China., Cai X; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China., Sun Z; Central Laboratory, Linyi Central Hospital, No.17 Jiankang Road, Linyi 276400, China., Cheng W; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China., Luo X; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China., Zhu HL; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China., Qian Y; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China.; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China.; Department of Radiology, Nanjing Drum Tower Hospital, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
المصدر: Analytical chemistry [Anal Chem] 2024 May 07; Vol. 96 (18), pp. 7005-7013. Date of Electronic Publication: 2024 Apr 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Hydrogen Sulfide*/analysis , Hydrogen Sulfide*/metabolism , Fluorescent Dyes*/chemistry , Fluorescent Dyes*/chemical synthesis , Arylamine N-Acetyltransferase*/metabolism , Carcinoma, Hepatocellular*/metabolism , Carcinoma, Hepatocellular*/pathology , Liver Neoplasms*/metabolism , Liver Neoplasms*/diagnostic imaging , Liver Neoplasms*/pathology , Photons*, Humans ; Animals ; Mice ; Hep G2 Cells ; Optical Imaging
مستخلص: Hydrogen sulfide (H 2 S), a critical gas signaling molecule, and N -acetyltransferase 2 (NAT2), a key enzyme in drug metabolism, are both known active biomarkers for liver function. However, the interactions and effects of H 2 S and NAT2 in living cells or lesion sites remain unknown due to the lack of imaging tools to achieve simultaneous detection of these two substances, making it challenging to implement real-time imaging and precise tracking. Herein, we report an activity-based two-photon fluorescent probe, TPSP-1 , for the cascade detection of H 2 S and NAT2 in living liver cells. Continuous conversion from TPSP-1 to TPSP-3 was achieved in liver cells and tissues. Significantly, leveraging the outstanding optical properties of this two-photon fluorescent probe, TPSP-1 , has been effectively used to identify pathological tissue samples directly from clinical liver cancer patients. This work provides us with this novel sensing and two-photon imaging probe, which can be used as a powerful tool to study the physiological functions of H 2 S and NAT2 and will help facilitate rapid and accurate diagnosis and therapeutic evaluation of hepatocellular carcinoma.
المشرفين على المادة: YY9FVM7NSN (Hydrogen Sulfide)
0 (Fluorescent Dyes)
EC 2.3.1.5 (Arylamine N-Acetyltransferase)
EC 2.3.1.5 (NAT2 protein, human)
تواريخ الأحداث: Date Created: 20240424 Date Completed: 20240507 Latest Revision: 20240529
رمز التحديث: 20240529
DOI: 10.1021/acs.analchem.4c00061
PMID: 38657082
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-6882
DOI:10.1021/acs.analchem.4c00061