دورية أكاديمية
A giant virus infecting the amoeboflagellate Naegleria.
| العنوان: | A giant virus infecting the amoeboflagellate Naegleria. |
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| المؤلفون: | Arthofer P; University of Vienna, Centre for Microbiology and Environmental Systems Science, Division of Microbial Ecology, Vienna, Austria.; University of Vienna, Doctoral School in Microbiology and Environmental Science, Vienna, Austria., Panhölzl F; University of Vienna, Centre for Microbiology and Environmental Systems Science, Division of Microbial Ecology, Vienna, Austria., Delafont V; Ecologie et Biologie des Interactions Laboratory (EBI), Microorganisms, hosts & environments team, Université de Poitiers, UMR CNRS, Poitiers, France., Hay A; Ecologie et Biologie des Interactions Laboratory (EBI), Microorganisms, hosts & environments team, Université de Poitiers, UMR CNRS, Poitiers, France., Reipert S; University of Vienna, Research Support Facilities UBB, Vienna, Austria., Cyran N; University of Vienna, Research Support Facilities UBB, Vienna, Austria., Wienkoop S; University of Vienna, Department of Functional and Evolutionary Ecology, Division of Molecular Systems Biology, Vienna, Austria., Willemsen A; University of Vienna, Centre for Microbiology and Environmental Systems Science, Division of Microbial Ecology, Vienna, Austria., Sifaoui I; Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, and Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Tenerife, Islas Canarias, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain., Arberas-Jiménez I; Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, and Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Tenerife, Islas Canarias, Spain., Schulz F; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, USA., Lorenzo-Morales J; Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, and Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Tenerife, Islas Canarias, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain., Horn M; University of Vienna, Centre for Microbiology and Environmental Systems Science, Division of Microbial Ecology, Vienna, Austria. matthias.horn@univie.ac.at. |
| المصدر: | Nature communications [Nat Commun] 2024 Apr 24; Vol. 15 (1), pp. 3307. Date of Electronic Publication: 2024 Apr 24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Genome, Viral*/genetics , Giant Viruses*/genetics , Giant Viruses*/classification , Giant Viruses*/ultrastructure , Giant Viruses*/isolation & purification , Giant Viruses*/physiology , Naegleria*/genetics , Naegleria*/virology, Naegleria fowleri/genetics ; Naegleria fowleri/isolation & purification ; Phylogeny ; Humans |
| مستخلص: | Giant viruses (Nucleocytoviricota) are significant lethality agents of various eukaryotic hosts. Although metagenomics indicates their ubiquitous distribution, available giant virus isolates are restricted to a very small number of protist and algal hosts. Here we report on the first viral isolate that replicates in the amoeboflagellate Naegleria. This genus comprises the notorious human pathogen Naegleria fowleri, the causative agent of the rare but fatal primary amoebic meningoencephalitis. We have elucidated the structure and infection cycle of this giant virus, Catovirus naegleriensis (a.k.a. Naegleriavirus, NiV), and show its unique adaptations to its Naegleria host using fluorescence in situ hybridization, electron microscopy, genomics, and proteomics. Naegleriavirus is only the fourth isolate of the highly diverse subfamily Klosneuvirinae, and like its relatives the NiV genome contains a large number of translation genes, but lacks transfer RNAs (tRNAs). NiV has acquired genes from its Naegleria host, which code for heat shock proteins and apoptosis inhibiting factors, presumably for host interactions. Notably, NiV infection was lethal to all Naegleria species tested, including the human pathogen N. fowleri. This study expands our experimental framework for investigating giant viruses and may help to better understand the basic biology of the human pathogen N. fowleri. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | P37198-B Austrian Science Fund (Fonds zur Förderung der Wissenschaftlichen Forschung); 891572 EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 Marie Skłodowska-Curie Actions (H2020 Excellent Science - Marie Skłodowska-Curie Actions); 101039843 EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) |
| تواريخ الأحداث: | Date Created: 20240424 Date Completed: 20240425 Latest Revision: 20240427 |
| رمز التحديث: | 20240427 |
| مُعرف محوري في PubMed: | PMC11043551 |
| DOI: | 10.1038/s41467-024-47308-2 |
| PMID: | 38658525 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-024-47308-2 |