دورية أكاديمية
أعرض في EDS

Skeletal muscle differentiation induces wide-ranging nucleosome repositioning in muscle gene promoters.

التفاصيل البيبلوغرافية
العنوان: Skeletal muscle differentiation induces wide-ranging nucleosome repositioning in muscle gene promoters.
المؤلفون: Harris S; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Anwar I; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Baksh SS; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Pratt RE; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Dzau VJ; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Hodgkinson CP; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA. conrad.hodgkinson@duke.edu.
المصدر: Scientific reports [Sci Rep] 2024 Apr 24; Vol. 14 (1), pp. 9396. Date of Electronic Publication: 2024 Apr 24.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Nucleosomes*/metabolism , Nucleosomes*/genetics , Promoter Regions, Genetic* , Cell Differentiation*/genetics , Muscle, Skeletal*/metabolism , Muscle Development*/genetics, Animals ; Mice ; Cell Line ; Sp3 Transcription Factor/metabolism ; Sp3 Transcription Factor/genetics ; Muscle Fibers, Skeletal/metabolism
مستخلص: In a previous report, we demonstrated that Cbx1, PurB and Sp3 inhibited cardiac muscle differentiation by increasing nucleosome density around cardiac muscle gene promoters. Since cardiac and skeletal muscle express many of the same proteins, we asked if Cbx1, PurB and Sp3 similarly regulated skeletal muscle differentiation. In a C2C12 model of skeletal muscle differentiation, Cbx1 and PurB knockdown increased myotube formation. In contrast, Sp3 knockdown inhibited myotube formation, suggesting that Sp3 played opposing roles in cardiac muscle and skeletal muscle differentiation. Consistent with this finding, Sp3 knockdown also inhibited various muscle-specific genes. The Cbx1, PurB and Sp3 proteins are believed to influence gene-expression in part by altering nucleosome position. Importantly, we developed a statistical approach to determine if changes in nucleosome positioning were significant and applied it to understanding the architecture of muscle-specific genes. Through this novel statistical approach, we found that during myogenic differentiation, skeletal muscle-specific genes undergo a set of unique nucleosome changes which differ significantly from those shown in commonly expressed muscle genes. While Sp3 binding was associated with nucleosome loss, there appeared no correlation with the aforementioned nucleosome changes. In summary, we have identified a novel role for Sp3 in skeletal muscle differentiation and through the application of quantifiable MNase-seq have discovered unique fingerprints of nucleosome changes for various classes of muscle genes during myogenic differentiation.
(© 2024. The Author(s).)
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معلومات مُعتمدة: No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation
فهرسة مساهمة: Keywords: Cardiac muscle; ChIP-seq; MNase-seq; Skeletal muscle; Sp3
المشرفين على المادة: 0 (Nucleosomes)
148710-94-5 (Sp3 Transcription Factor)
تواريخ الأحداث: Date Created: 20240424 Date Completed: 20240424 Latest Revision: 20240427
رمز التحديث: 20240427
مُعرف محوري في PubMed: PMC11043329
DOI: 10.1038/s41598-024-60236-x
PMID: 38658615
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-60236-x