دورية أكاديمية
Skeletal muscle differentiation induces wide-ranging nucleosome repositioning in muscle gene promoters.
العنوان: | Skeletal muscle differentiation induces wide-ranging nucleosome repositioning in muscle gene promoters. |
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المؤلفون: | Harris S; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Anwar I; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Baksh SS; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Pratt RE; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Dzau VJ; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA., Hodgkinson CP; Mandel Center for Heart and Vascular Research, The Duke Cardiovascular Research Center, Duke University Medical Center, Duke University, CaRL Building, 213 Research Drive, Durham, NC, 27710, USA. conrad.hodgkinson@duke.edu. |
المصدر: | Scientific reports [Sci Rep] 2024 Apr 24; Vol. 14 (1), pp. 9396. Date of Electronic Publication: 2024 Apr 24. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Nucleosomes*/metabolism , Nucleosomes*/genetics , Promoter Regions, Genetic* , Cell Differentiation*/genetics , Muscle, Skeletal*/metabolism , Muscle Development*/genetics, Animals ; Mice ; Cell Line ; Sp3 Transcription Factor/metabolism ; Sp3 Transcription Factor/genetics ; Muscle Fibers, Skeletal/metabolism |
مستخلص: | In a previous report, we demonstrated that Cbx1, PurB and Sp3 inhibited cardiac muscle differentiation by increasing nucleosome density around cardiac muscle gene promoters. Since cardiac and skeletal muscle express many of the same proteins, we asked if Cbx1, PurB and Sp3 similarly regulated skeletal muscle differentiation. In a C2C12 model of skeletal muscle differentiation, Cbx1 and PurB knockdown increased myotube formation. In contrast, Sp3 knockdown inhibited myotube formation, suggesting that Sp3 played opposing roles in cardiac muscle and skeletal muscle differentiation. Consistent with this finding, Sp3 knockdown also inhibited various muscle-specific genes. The Cbx1, PurB and Sp3 proteins are believed to influence gene-expression in part by altering nucleosome position. Importantly, we developed a statistical approach to determine if changes in nucleosome positioning were significant and applied it to understanding the architecture of muscle-specific genes. Through this novel statistical approach, we found that during myogenic differentiation, skeletal muscle-specific genes undergo a set of unique nucleosome changes which differ significantly from those shown in commonly expressed muscle genes. While Sp3 binding was associated with nucleosome loss, there appeared no correlation with the aforementioned nucleosome changes. In summary, we have identified a novel role for Sp3 in skeletal muscle differentiation and through the application of quantifiable MNase-seq have discovered unique fingerprints of nucleosome changes for various classes of muscle genes during myogenic differentiation. (© 2024. The Author(s).) |
References: | Turk J Emerg Med. 2018 Aug 07;18(3):91-93. (PMID: 30191186) J Muscle Res Cell Motil. 2000 Feb;21(2):153-69. (PMID: 10961839) Circ Res. 2017 Apr 28;120(9):1403-1413. (PMID: 28209718) Genome Res. 2013 Feb;23(2):341-51. (PMID: 23193179) PLoS One. 2017 Aug 7;12(8):e0182771. (PMID: 28787030) J Biol Chem. 1999 Jul 9;274(28):19573-80. (PMID: 10391891) Genome Biol. 2019 Sep 13;20(1):198. (PMID: 31519205) Nucleic Acids Res. 2019 Oct 10;47(18):9511-9523. (PMID: 31504766) Nat Commun. 2016 May 06;7:11485. (PMID: 27151365) Cold Spring Harb Perspect Biol. 2010 Nov;2(11):a003996. (PMID: 20961976) Gene. 2016 Jan 15;576(1 Pt 3):385-94. (PMID: 26526134) Stem Cells. 2018 Aug;36(8):1198-1209. (PMID: 29676038) Pharmacol Rev. 2015 Oct;67(4):821-70. (PMID: 26362469) J Biol Chem. 2022 Jun;298(6):102053. (PMID: 35605661) Cancer Res. 1999 Sep 1;59(17):4266-70. (PMID: 10485470) BMC Genomics. 2015 Jun 25;16:475. (PMID: 26109061) Methods Mol Biol. 2017;1446:189-205. (PMID: 27812944) Bioinformatics. 2011 Aug 1;27(15):2149-50. (PMID: 21653521) Sci Rep. 2016 Jan 21;6:19620. (PMID: 26790608) J Exp Orthop. 2016 Dec;3(1):15. (PMID: 27447481) EMBO J. 1996 Oct 15;15(20):5659-67. (PMID: 8896459) Biomed Res Int. 2018 Oct 4;2018:8953079. (PMID: 30402496) Am J Physiol Cell Physiol. 2023 Apr 1;324(4):C843-C855. (PMID: 36847443) J Biol Chem. 2003 Sep 12;278(37):35775-80. (PMID: 12837748) Front Pharmacol. 2016 Jan 14;6:314. (PMID: 26834636) STAR Protoc. 2021 May 18;2(2):100486. (PMID: 34041500) Mol Cell Biol. 2004 Dec;24(24):10777-91. (PMID: 15572681) |
معلومات مُعتمدة: | No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation; No Agency ID Edna and Fred L. Mandel, Jr. Foundation |
فهرسة مساهمة: | Keywords: Cardiac muscle; ChIP-seq; MNase-seq; Skeletal muscle; Sp3 |
المشرفين على المادة: | 0 (Nucleosomes) 148710-94-5 (Sp3 Transcription Factor) |
تواريخ الأحداث: | Date Created: 20240424 Date Completed: 20240424 Latest Revision: 20240427 |
رمز التحديث: | 20240427 |
مُعرف محوري في PubMed: | PMC11043329 |
DOI: | 10.1038/s41598-024-60236-x |
PMID: | 38658615 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-024-60236-x |